Electrochemical detection of ctDNA mutation in non-small cell lung cancer based on CRISPR/Cas12a system

Feng, Lijuan; Peng, Jun; Lei, Youming; Liu, Rongsheng; Jin, Lian; Liang, Huan; Liu, Huifang; Ma, Si-Yin; Zhang, Xiaohua; Zhang, Yaping; Li, Can-Peng; Zhao, Hui

doi:10.1016/j.snb.2022.131807

Cited by 40 publications

(17 citation statements)

References 46 publications

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“…Liu et al [ 46 ] developed a rapid, accurate, and cost-effective assay for the detection of ctDNA EGFR L858R based on the CRISPR/Cas12a system and MB/Fe 3 O 4 @COF/PdAu nanocomposites. The schematic of this approach is shown in Figure 7 .…”

Section: Nucleic Acid Probe-based Detectionmentioning

confidence: 99%

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Electrochemical Biosensors for Circulating Tumor DNA Detection

et al. 2022

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Early diagnosis and treatment have always been highly desired in the fight against cancer, and detection of circulating tumor DNA (ctDNA) has recently been touted as highly promising for early cancer-screening. Consequently, the detection of ctDNA in liquid biopsy is gaining much attention in the field of tumor diagnosis and treatment, which has also attracted research interest from industry. However, it is difficult to achieve low-cost, real-time, and portable measurement of ctDNA in traditional gene-detection technology. Electrochemical biosensors have become a highly promising solution to ctDNA detection due to their unique advantages such as high sensitivity, high specificity, low cost, and good portability. Therefore, this review aims to discuss the latest developments in biosensors for minimally invasive, rapid, and real-time ctDNA detection. Various ctDNA sensors are reviewed with respect to their choices of receptor probes, designs of electrodes, detection strategies, preparation of samples, and figures of merit, sorted by type of electrode surface recognition elements. The development of biosensors for the Internet of Things, point-of-care testing, big data, and big health is analyzed, with a focus on their portable, real-time, and non-destructive characteristics.

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Section: Nucleic Acid Probe-based Detectionmentioning

confidence: 99%

“… Schematic diagram of ctDNA electrochemical biosensor based on CRISPR/Cas12a (Reprinted with permission from Ref. [ 46 ]. 2022, Liu et al).…”

Section: Figurementioning

confidence: 99%

Electrochemical Biosensors for Circulating Tumor DNA Detection

et al. 2022

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“…The recognized sequence is usually cleaved which offers the possibility to use reporter-quencher technology with consequent fluorescence measurement [136]. CRISPR/Cas system is often used for microRNA detection [137] or is coupled to various amplification technique such as PCR [71,72], RPA [82], or ESDR (described above) [81]. Compared to optical techniques, EC methods are less frequently combined with the CRISPR/ Cas, but this combination has a huge potential that should be undoubtedly exploited.…”

Section: Ec Biosensors Without Enzymatic Amplificationmentioning

confidence: 99%

“…Unmutated (wild-type) amplicons remained uncleaved and were bound to a capture probe immobilized on the gold electrode surface. In the presence of an uncleaved amplicon, second capture probe was introduced that was immobilized on covalent organic frameworks covered with PdAu nanosheets and methylene blue to produce EC signal [82]. A very interesting study has been reported by Balderston et al who discriminated single-point mutations in unamplified genomic DNA via CRISPR-associated protein Cas9 immobilized on a graphene field-effect transistor [138].…”

Section: Ec Biosensors Without Enzymatic Amplificationmentioning

confidence: 99%

Electrochemical biosensors for analysis of DNA point mutations in cancer research

et al. 2022

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Cancer is a genetic disease induced by mutations in DNA, in particular point mutations in important driver genes that lead to protein malfunctioning and ultimately to tumorigenesis. Screening for the most common DNA point mutations, especially in such genes as TP53, BRCA1 and BRCA2, EGFR, KRAS, or BRAF, is crucial to determine predisposition risk for cancer or to predict response to therapy. In this review, we briefly depict how these genes are involved in cancer, followed by a description of the most common techniques routinely applied for their analysis, including high-throughput next-generation sequencing technology and less expensive low-throughput options, such as real-time PCR, restriction fragment length polymorphism, or high resolution melting analysis. We then introduce benefits of electrochemical biosensors as interesting alternatives to the standard methods in terms of cost, speed, and simplicity. We describe most common strategies involved in electrochemical biosensing of point mutations, relying mostly on PCR or isothermal amplification techniques, and critically discuss major challenges and obstacles that, until now, prevented their more widespread application in clinical settings.

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“…Notably, noble metal particles were suitable as modi ed electrode materials, because of their high conductivity and catalytic activity. Additionally, bimetallic nanomaterials have higher speci c surface area and synergy, better catalysis, conductivity and luminescence than single metal nanoparticles, and are widely used in the eld of sensors [24]. Lu et al [25] synthesized onedimensional bimetallic Pd@Au core-shell nanorods and constructed a highly sensitive AChE biosensor for the detection of OPs.…”

Section: Introductionmentioning

confidence: 99%