Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Surkus, Annette‐Enrica; Flechsig, Gerd‐Uwe

doi:10.1002/elan.200804539

Cited by 30 publications

(23 citation statements)

References 32 publications

(19 reference statements)

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“…Flechsig et al measured a signal around -0.29 V due to osmium-modified DNA targets after their hybridization with end-thiolated capture probes anchored at gold electrodes [8,58], including rotating and electrically heated ones [75]. This concept has been utilized in several interesting applications, including studies of temperature effects on the DNA hybridization [75], analysis of DNA melting curves [60] and various polymerase chain reaction (PCR)-coupled electrochemical assays of nucleotide sequences [58,59,76,77] (see section 5.2).…”

Section: Gold Electrodesmentioning

confidence: 97%

Osmium Tetroxide Complexes as Versatile Tools for Structure Probing and Electrochemical Analysis of Biopolymers

Fojta¹,

Kostečka²,

Pivoňková³

et al. 2011

CAC

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Osmium tetroxide complexes with nitrogenous ligands and analogous complexes of six-valent osmium proved excellent tools for selective labeling of biopolymers (nucleic acids, proteins and polysaccharides). Reactions of these species with target moieties within the biopolymer molecules (pyrimidine nucleobases, tryptophan residues or sugar moieties) are facile at physiological conditions and are in general structure-selective, allowing their application in DNA and protein structure probing. The modification products can be detected by a variety of widely accessible analytical techniques, including biochemical (enzymatic) approaches, immunoassays, chemical DNA sequencing, spectrophotometry and electrochemistry. Particularly the electrochemical techniques are promising for utilization in biosensors and routine bioassays due to the possibility of highly sensitive and selective detection of the labeled biopolymers adducts based on distinct electrochemical properties of the introduced osmium moieties. Utilization of the osmium tags in probing DNA structural transitions, sensing of DNA hybridization, damage and DNA methylation, labeling of peptides and proteins, probing accessibility of tryptophan residues in proteins and their complexes, and labeling of sugar moieties, are reviewed.

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Section: Gold Electrodesmentioning

confidence: 97%

Osmium Tetroxide Complexes as Versatile Tools for Structure Probing and Electrochemical Analysis of Biopolymers

Fojta¹,

Kostečka²,

Pivoňková³

et al. 2011

CAC

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“…Fluorescence‐based, solution‐phase methods such as dynamic allele‐specific hybridization (DASH) can, in contrast, distinguish homozygous and heterozygous SNPs by means of melting‐curve analysis in solution phase 20. This has spurred interest in integrating melting‐curve analysis with electrochemical detection, but these efforts have been confounded by difficulties in obtaining accurate electrochemical and temperature measurements with sufficient speed and resolution 21–23…”

Section: Apoe Genotypes and Their Associated Microe‐dash Readoutmentioning

confidence: 99%

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

et al. 2014

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We report the first electrochemical system for the detection of single‐nucleotide polymorphisms (SNPs) that can accurately discriminate homozygous and heterozygous genotypes using microfluidics technology. To achieve this, our system performs real‐time melting‐curve analysis of surface‐immobilized hybridization probes. As an example, we used our sensor to analyze two SNPs in the apolipoprotein E (ApoE) gene, where homozygous and heterozygous mutations greatly affect the risk of late‐onset Alzheimer’s disease. Using probes specific for each SNP, we simultaneously acquired melting curves for probe–target duplexes at two different loci and thereby accurately distinguish all six possible ApoE allele combinations. Since the design of our device and probes can be readily adapted for targeting other loci, we believe that our method offers a modular platform for the diagnosis of SNP‐based diseases and personalized medicine.

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“…We also found a dramatic effect of elevated temperature during DNA hybridization at a directly heated probe-modified gold electrode [35]. This approach allows electrochemical DNA melting curve analysis [36].…”

Section: Introductionmentioning

confidence: 98%

Directly Heated Bismuth Film Electrodes Based on Gold Microwires

et al. 2010

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As a nontoxic substitute for mercury electrodes, bismuth electrodes attained a lot of attention during the last years. In this report we describe for the first time the preparation of two different directly heatable bismuth-modified microwire electrodes. We characterized the electrochemical behaviour using cyclic voltammetry in acetate buffer and alkaline tartrate solution. The bismuth electrodes show a significantly wider potential window compared with bare gold wires. In the presence of picric acid as one example for the detection of explosives, the bismuth electrodes deliver higher signals. By applying heat during the measurements, the signals can be enhanced further. We used the temperature pulse amperometry (TPA) technique to improve the electrochemical response at the different types of electrodes. In this preliminary study, we were able to detect 3 ppm traces of picric acid.

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Electrochemical Detection of DNA Melting Curves by Means of Heated Biosensors

Cited by 30 publications

References 32 publications

Osmium Tetroxide Complexes as Versatile Tools for Structure Probing and Electrochemical Analysis of Biopolymers

Osmium Tetroxide Complexes as Versatile Tools for Structure Probing and Electrochemical Analysis of Biopolymers

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

Directly Heated Bismuth Film Electrodes Based on Gold Microwires

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