Electrochemical Detection of Electrolytes Using a Solid-State Ion-Selective Electrode of Single-Piece Type Membrane

Chen, Li-Da; Wang, Wei-Jhen; Wang, Gou‐Jen

doi:10.3390/bios11040109

Cited by 20 publications

(6 citation statements)

References 42 publications

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“…As shown in Table 1 and Table 2 , measurement range and sensitivities of DWE were comparable or superior compared with those of recently developed glucose and lactate meters. These successful measurements across a wide range of concentrations of each metabolite (0.5–35 mM for glucose and 0.5–25 mM for lactate) indicated that the proposed DEB is suitable to quantify both glucose and lactate concentrations, spanning their physiological range in mammals (0.56–35 mM for glucose and 0.5–25 mM for lactate) [ 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood

2021

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Understanding the levels of glucose (G) and lactate (L) in blood can help us regulate various chronic health conditions such as obesity. In this paper, we introduced an enzyme-based electrochemical biosensor adopting glucose oxidase and lactate oxidase on two working screen-printed carbon electrodes (SPCEs) to sequentially determine glucose and lactate concentrations in a single drop (~30 µL) of whole blood. We developed a diet-induced obesity (DIO) mouse model for 28 weeks and monitored the changes in blood glucose and lactate levels. A linear calibration curve for glucose and lactate concentrations in ranges from 0.5 to 35 mM and 0.5 to 25 mM was obtained with R-values of 0.99 and 0.97, respectively. A drastic increase in blood glucose and a small but significant increase in blood lactate were seen only in prolonged obese cases. The ratio of lactate concentration to glucose concentration (L/G) was calculated as the mouse’s gained weight. The results demonstrated that an L/G value of 0.59 could be used as a criterion to differentiate between normal and obesity conditions. With L/G and weight gain, we constructed a diagnostic plot that could categorize normal and obese health conditions into four different zones. The proposed dual electrode biosensor for glucose and lactate in mouse whole blood showed good stability, selectivity, sensitivity, and efficiency. Thus, we believe that this dual electrode biosensor and the diagnostic plot could be used as a sensitive analytical tool for diagnosing glucose and lactate biomarkers in clinics and for monitoring obesity.

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Section: Resultsmentioning

confidence: 99%

A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood

2021

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“…Considering this, cyclic voltammetry can further be used to establish a possible relation with the metal ion concentration based on Fenton reaction 35 . While the EIS data exhibited evidence for the correlation between impedance and metal ion/particle content, cyclic voltammetry failed to reveal solid inferences 23 . The serum standardization did not show any oxidation or reduction peaks.…”

Section: Cyclic Voltammetry Of Serum With Metalmentioning

confidence: 94%

Electrochemical Biosensor to Detect Implant Derived Metal Ions: A Mice Model

Gokul

Catton

Cheng

et al. 2022

Preprint

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Joint replacement surgeries are progressing at an increased rate in the United States, with around 1 million surgeries being performed annually. While these orthopedic implants provide a solution for a damaged joint, debris such as metal ions generated due to the wear and corrosion of the implants prove to be of considerable risk of systemic and local toxicity. As an effective diagnostic tool, there is a need to develop a patient-friendly, cost-effective, and rapid mode of metal ion detection in orthopedic patients with various implants. In this study, an electrochemical biosensor chip (Dropsens) is proposed as a diagnostic tool to detect metal ions through changes in impedance. In vivo studies were conducted using CoCrMo particles intra-particularly injected into mice. Then, blood was collected on 0th day, 14th day, and 28th day after injection and was centrifuged to obtain serum. The serum was subject to various electrochemical techniques such as Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV). Additionally, standardization experiments were performed using different concentrations of CoCrMo wear particles suspended in PBS and mice serum. The EIS standardization experiments reveal a negative correlation between impedance (Rp) value obtained after fitting with the equivalent CPE model and metal ion concentration in mice serum. A regression equation was obtained from the standard experiments. The CV data for differing metal ion concentrations and the regression equation were also obtained. The mice model data, the bode plot which depicts the change in impedance and phase angle at each frequency, the positive control group shows the highest impedance at any frequency followed by the experimental 28th day mice serum. The % change in impedance between the different groups revealed the increasing presence of metal ions in serum on 28th day. The CV experimental data obtained reveal a possible correlation between CV area and metal ion concentrations. Experimental mice models were established to mimic the possible diffusion of metal particles from implants. Dropsens electrochemical biosensors were efficiently employed to detect metal ions in serum through changes in impedance.

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“…Electrochemical tests that were employed are Open Circuit Potential (OCP), Electrical impedance spectroscopy (EIS), and Cyclic Voltammetry (CV). In addition, a calibration curve of current vs. concentration was generated using the cyclic voltammetry data 23 .…”

Section: Electrochemical Testsmentioning

confidence: 99%

Electrochemical Biosensor to Detect Implant-Derived Metal Ions: A Mice Model

et al. 2023

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Joint replacement surgeries are progressing at an increased rate in the United States, with around 1 million surgeries being performed annually. While these orthopedic implants provide a solution for a damaged joint, debris such as metal ions generated due to the wear and corrosion of the implants prove to be of considerable risk of systemic and local toxicity. As an effective diagnostic tool, there is a need to develop a patient-friendly, cost-effective, and rapid mode of metal ion detection in orthopedic patients with various implants.In this study, an electrochemical biosensor chip (Dropsens) is proposed as a diagnostic tool to detect metal ions through changes in impedance. In vivo studies were conducted using CoCrMo particles intraparticularly injected into mice. Then, blood was collected on 0th day, 14th day, and 28th day after injection and was centrifuged to obtain serum. The serum was subject to various electrochemical techniques such as Electrochemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV). Additionally, standardization experiments were performed using different concentrations of CoCrMo wear particles suspended in PBS and mice serum.The EIS standardization experiments reveal a negative correlation between impedance (Rp) value obtained after tting with the equivalent CPE model and metal ion concentration in mice serum. A regression equation was obtained from the standard experiments. The CV data for differing metal ion concentrations and the regression equation were also obtained. The mice model data, the bode plot which depicts the change in impedance and phase angle at each frequency, the positive control group shows the highest impedance at any frequency followed by the experimental 28th day mice serum. The % change in impedance between the different groups revealed the increasing presence of metal ions in serum on 28th day. The CV experimental data obtained reveal a possible correlation between CV area and metal ion concentrations. Experimental mice models were established to mimic the possible diffusion of metal particles from implants. Dropsens electrochemical biosensors were e ciently employed to detect metal ions in serum through changes in impedance.

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Electrochemical Detection of Electrolytes Using a Solid-State Ion-Selective Electrode of Single-Piece Type Membrane

Cited by 20 publications

References 42 publications

A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood

A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood

Electrochemical Biosensor to Detect Implant Derived Metal Ions: A Mice Model

Electrochemical Biosensor to Detect Implant-Derived Metal Ions: A Mice Model

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