Electrochemical Detection of Glutathione Using a Poly(caffeic acid) Nanocarbon Composite Modified Electrode

Abstract

A modified glassy carbon electrode was prepared through electropolymerization of caffeic acid in the presence of either carbon nanotubes or nano‐carbon drop cast onto the electrode surface. The voltammetric behaviour of the electrode was characterized using the ortho‐quinone moiety on the caffeic acid unit and the surface loading optimized for current response. The nanocomposite mediated electrode was used for the sensitive detection of glutathione at concentrations as low as 500 nM.

Development of an Electrochemical Sensor for NADH Determination Based on a Caffeic Acid Redox Mediator Supported on Carbon Black

“…In this respect, it has to be evidenced that the electrochemical deposition is the procedure generally reported by the literature [5][6][7]25]. However, by considering that the mechanisms proposed for CFA deposition on carbon surfaces are based on Michael addition, the drop-casting method represents an alternative deposition approach, which gives the CFAads/CB/SPE sensor system.…”

Selective electrochemical detection of thiol biomarkers in saliva using multiwalled carbon nanotube screen-printed electrodes

“…One common type of point-to-care sensors is that based on carbon screen-printed electrode [20][21][22][23]. It is best suited for applications that require high throughput testing, such as clinical testing [22][23][24]. To the best of our knowledge, there is no literature reporting thiol detection in saliva without the use of expensive instrumentation or elaborate separation technique.…”

“…The voltammogram for this type of reaction typically shows an increase in forward peak and a decrease in the backward peak as the concentration of thiol compound is increased (Scheme 1a) [20,24]. The second possible pathway is through a 1,4-Michael addition reaction.…”

Au nanoparticles/poly(caffeic acid) composite modified glassy carbon electrode for voltammetric determination of acetaminophen

“…Owing to the importance of naturally occurring o-quinones in the processing of mediated electron transfer reactions, PCA modified electrode has been found to facilitate in the direct electron-transfer between the electrode and the target biological molecules, such as nicotinamide adenine dinucleotide (NADH) [6,9], AP [7], epinephrine [9], uric acid [10], dopamine [11], ascorbic acid [12] and their mixture.…”

“…Therefore, electropolymerization is a promising alternative to prepare PCA modified electrode as adjusting the electrochemical parameters can control film thickness, permeation and charge transport characteristics [5]. Electropolymerization of PCA can be carried out in potentiostatic (constant potential) method [6] and potentiodynamic (cyclic voltammetric) method [7]. Compared with voltammetric method, the constant potential method performed under a stable electrode potential exhibits some superiority because it can effectively hamper the side reactions and yield polymers with regular structure, high electroactivity, and purity.…”