Electrochemical detection of matrix metalloproteinase-7 using an immunoassay on a methylene blue/2D MoS2/graphene oxide electrode

Yaiwong, Patrawadee; Semakul, Natthawat; Jakmunee, Jaroon

doi:10.1016/j.bioelechem.2021.107944

Cited by 24 publications

(17 citation statements)

References 65 publications

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“…Peptide decorated gold-CNT electrode recognizes the MMP-7 protein, presenting linear detection from 0.01 to 1000 ng⋅mL −1 and LOD of 6 pg⋅mL −1 [ 73 ]. A screen-printed electrode was designed by grafting antibodies into 2D structures, graphene oxide and MoS 2 , aiming to amplify the analytical signal for the detection of MMP-7 [ 74 ]. Indeed, the 2D nanostructured immunosensor showed improvements and presented range of detection from 10 pg.mL −1 to 75 ng⋅mL −1 and LOD of 7 pg⋅mL −1 .…”

Section: Resultsmentioning

confidence: 99%

Detecting the PEX Like Domain of Matrix Metalloproteinase-14 (MMP-14) with Therapeutic Conjugated CNTs

et al. 2022

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Matrix metalloproteinases (MMPs) are essential proteins acting directly in the breakdown of the extra cellular matrix and so in cancer invasion and metastasis. Given its impact on tumor angiogenesis, monitoring MMP-14 provides strategic insights on cancer severity and treatment. In this work, we report a new approach to improve the electrochemical interaction of the MMP-14 with the electrode surface while preserving high specificity. This is based on the detection of the hemopexin (PEX) domain of MMP-14, which has a greater availability with a stable and low-cost commercial molecule, as a recognition element. This molecule, called NSC-405020, is specific of the PEX domain of MMP-14 within the binding pocket. Through the covalent grafting of the NSC-405020 molecule on carbon nanotubes (CNTs), we were able to detect and quantify MMP-14 using electrochemical impedance spectroscopy with a linear range of detection of 10 ng⋅mL−1 to 100 ng⋅mL−1, and LOD of 7.5 ng⋅mL−1. The specificity of the inhibitory small molecule was validated against the PEX domain of MMP-1. The inhibitor loaded CNTs system showed as a desirable candidate to become an alternative to the conventional recognition bioelements for the detection of MMP-14.

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Section: Resultsmentioning

confidence: 99%

Detecting the PEX Like Domain of Matrix Metalloproteinase-14 (MMP-14) with Therapeutic Conjugated CNTs

et al. 2022

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“…They possess atomically layered structures, large surface areas, and outstanding electrochemical characteristics (Jing et al, 2014;Rohaizad et al, 2017). In their uses in electrochemical sensors, 2D materials can not only improve electrode reactivity but also load chemicals and biochemicals at high contents because of unique electrochemical properties and massive electroactive surface areas (Yaiwong et al, 2021;Pothipor et al, 2022). They are getting a lot of interest as both electrode modifiers (Rohaizad et al, 2017;Yaiwong et al, 2021) and tagging materials (Hong et al, 2020) for immunosensors.…”

Section: Introductionmentioning

confidence: 99%

“…In their uses in electrochemical sensors, 2D materials can not only improve electrode reactivity but also load chemicals and biochemicals at high contents because of unique electrochemical properties and massive electroactive surface areas (Yaiwong et al, 2021;Pothipor et al, 2022). They are getting a lot of interest as both electrode modifiers (Rohaizad et al, 2017;Yaiwong et al, 2021) and tagging materials (Hong et al, 2020) for immunosensors. They also present fast heterogeneous electron-transfer rates (Rohaizad et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Chanarsa

Jakmunee

Ounnunkad

2022

Front. Cell. Infect. Microbiol.

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Liver cancer is one of the most common global health problems that features a high mortality rate. Alpha-fetoprotein (AFP) is a potential liver cancer biomarker for the diagnosis of liver cancer. The quantitative detection of AFP at an ultratrace level has important medical significance. Using the reaction of the antibody–antigen pair in an immunosensor enables the sensitive and selective AFP assay. Finding a strategy in signal generation and amplification is challenging to fabricate new sensitive electrochemical immunosensors for AFP detection. This study demonstrates the construction of a simple, reliable, and label-free immunosensor for the detection of AFP on a smart phone. Exfoliated two-dimensional (2D) molybdenum diselenide (MoSe2) and 2D tungsten diselenide (WSe2) were employed to modify the disposable screen-printed carbon electrode (SPCE) to use as the electrochemical platform, which is affixed to a small potentiostat connected to a smart phone. The modified electrode offers antibody immobilization and allows detection of AFP via an immunocomplex forming a sandwich-like configuration with the AFP-corresponding aptamer. A heterojunction 2D MoSe2/2D WSe2 composite improves the SPCE’s reactivity and provides a large surface area and good adsorption capacity for the immobilizing antibodies. The signal generation for the immunosensor is from the electrochemical response of methylene blue (MB) intercalating into the aptamer bound on the electrode. The response for the proposed sandwich-like immunosensor is proportional to the AFP concentration (1.0–50,000 pg ml-1). The biosensor has potential for the development of a simple and robust point-of-care diagnostic platform for the clinical diagnosis of liver cancer, achieving a low limit of detection (0.85 pg ml-1), high sensitivity, high selectivity, good stability, and excellent reproducibility.

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“…[30][31][32] Graphene oxide and MB-based nanocomposites have been investigated for their biosensing activity. [33,34] MB dye has been a well-known probe molecule to investigate SERS strength of metallic nanoparticle based substrates. [2,[35][36][37][38] Hao et al have investigated GERS signal of MB showing significant influence of applied electric field and chemical doping on graphene, where a higher effect due to chemical doping than field-effect was observed.…”

Section: Introductionmentioning

confidence: 99%

“…[ 30–32 ] Graphene oxide and MB‐based nanocomposites have been investigated for their biosensing activity. [ 33,34 ] MB dye has been a well‐known probe molecule to investigate SERS strength of metallic nanoparticle based substrates. [ 2,35–38 ] Hao et al.…”

Section: Introductionmentioning

confidence: 99%

Toward Graphene‐Enhanced Spectroelectrochemical Sensors

Kaushik

Sonia

Haider

et al. 2022

Adv Materials Inter

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The SERS effect arises mainly from electromagnetic (EM) and chemical (CM) enhancement of the Raman signal. [3] The EM enhancement is on the order of ≈|E| 4 , amplifying signals >10 8 , where E is the intensity of the EM field. On the other hand, CM enhancement has been shown to enhance the signal ≈10-100×. Nevertheless, SERS enhancement is contingent on the quality of the substrate. Preparation of a suitable substrate requires rigorous micro/nanofabrication processes, such as electrochemical or high vacuum deposition of noble metals (e.g., Ag, Au, Cu) or the selfassembly of size-controlled colloidal noble metal nanoparticles, which are very complex, contaminated, and not homogenous, leading to a lowered SERS effect. Moreover, when metal-based rough substrates, such as Ag or Cu, are used for SERS, they tend to oxidize, which prevents their use in some applications. Additionally, noble metals have relatively poor biological compatibility, hindering their use in biomedical applications.Recently, graphene-enhanced Raman spectroscopy (GERS) has emerged as a novel technique where graphene-based ultra-smooth substrates exhibit strong Raman enhancement from adsorbed molecules. [4][5][6] Graphene is cheap, easy to produce, and remains stable even in relatively harsh conditions. The flat and chemically inert surface and environmentally friendly nature of graphene make it compatible for widespread use including biosensing, biomedical, and environmental applications. [7][8][9] The GERS technique is associated with the CM enhancement mechanism, where the charge transfer occurs between adsorbed molecules and the substrate. [4,10] As the surface plasmon on graphene is in the terahertz range rather than the visible range, graphene does not support EM enhancement by the excitation of visible energy photons. [11] Chemical mechanism-initiated GERS facilitates molecular selectivity, enhancing the charge transfer between the molecules and the graphene. The high stability and reproducibility of the Raman enhanced spectra from graphene makes GERS more suitable for applications than SERS. [12] It has been previously reported that the enhancement from GERS on nitrogen-doped graphene was about ten times higher than SERS with the same probe molecules, while for pristine graphene, it was five times higher. [13] Different molecules have been probed with GERS, making it an effective approach for sensing. [14,15] Theoretical and experimental studies have been conducted to obtain more insight into the mechanism

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Electrochemical detection of matrix metalloproteinase-7 using an immunoassay on a methylene blue/2D MoS2/graphene oxide electrode

Cited by 24 publications

References 65 publications

Detecting the PEX Like Domain of Matrix Metalloproteinase-14 (MMP-14) with Therapeutic Conjugated CNTs

Detecting the PEX Like Domain of Matrix Metalloproteinase-14 (MMP-14) with Therapeutic Conjugated CNTs

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Toward Graphene‐Enhanced Spectroelectrochemical Sensors

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