Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Meriluoto, Jussi; Kincaid, Brendan J.; Smyth, Malcolm R.; Wasberg, M.

doi:10.1016/s0021-9673(98)00203-9

Cited by 42 publications

(20 citation statements)

References 29 publications

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“…Biochemical methods such as enzyme-linked immunosorbent assay (ELISA) [18][19][20] and the protein phosphatase inhibition assay [5,21] are useful as screening methods due to their high sensitivity and high throughput, but they often have poor identification ability and the potential for false-positives. Reversedphase high-performance liquid chromatography (HPLC) with UV detection [20], diode array detection [22][23][24][25][26][27] or electrochemical detection [28], LC-mass spectrometry (LC-MS) [29][30][31], capillary electrophoresis (CE) [30], and CE-MS [30] have also been used for the identification and quantification of MCs. The LC method can be much more definitive than bioassays and biochemical methods 0003 since it can provide information for the identification of individual MCs.…”

Section: Introductionmentioning

confidence: 99%

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Xiao

et al. 2009

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Xiao

et al. 2009

Analytica Chimica Acta

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“…This derivatized product is subject to sensitive fluorimetric HPLC quantification, analyte recovery, repeatability and detection limit achieved 89%, 6.2% and 120 ng-DA/L, respectively (Table 3). There were also other studies aimed at improved or novel HPLC methods including the use of amperometric HPLC [71], capillary electrophoresis (CE) and capillary electrochromatography (CEC) for the analysis of DSP, ASP, and MCs [72].…”

Section: Recent Development In Instrumental Analysis Of Algal Toxinsmentioning

confidence: 99%

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

2014

J Environ Anal Chem

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The detection and monitoring techniques for algal toxins and the causative harmful algal blooms (HABs) are essential for the protection of aquatic lives, shellfish safety, drinking water quality, and public health. Toward the development of fast, easy, and reliable techniques, much progress has been made during the last decade for the qualitative and quantitative analysis of algal toxins. This review highlights the recent progress and new trends of these analytical and monitoring tools, ranging from in-situ quick screening protocols for the monitoring of algal blooms to mass spectrometric analysis of trace levels of various algal toxins and structural elucidation. Solid-phase adsorption toxin tracking (SPATT) deployed in the field for the passive sampling of algal toxins has been recently validated, and improved ELISA-based methods with lower detection limits for more toxins have become commercially available for both screening and routine monitoring purposes. Liquid chromatography-mass spectrometry with several recent mass spectrometric innovations has expanded our understanding of traditional toxins, their metabolites along with newly discovered toxins of ecological importance. Several established in vivo and in vitro bioassays will continue to be used as benchmark toxicological testing of algal toxins; however, newly emerged molecular probing techniques such as real-time quantitative polymerase chain reaction (qPCR) have extended our ability to trace algal toxins from causative organisms at the molecular level. New chemical and biological sensors, lab-on-chip and remote sensing of blooms being developed will hold promise for early warning and routine monitoring to better manage and protect our freshwater, coastal and marine resources from adverse impact by harmful algal blooms.

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“…Both isocratic and gradient elution are equally employed, usually with the addition of 0.05-0.1% TFA acting as pH modifier and ion-pairing reagent. For isocratic elution, eluents such as water with ACN containing TFA [29,30], or formic acid [25], ammonium acetate with ACN [31,32] and phosphate buffer with methanol [33,34] or ACN [26,35] have been reported. In gradient elution, most often ACN gradient is employed [18,19,27,22,23,36,37], and also the use of methanol gradient was recently reported for LC/MS/MS systems [20,38] including ultra-performance LC/MS/MS system where a gradient elution and a mobile phases consisting of 0.1% formic acid in water and methanol were reported [21].…”

Section: Hplc Of MCmentioning

confidence: 99%

Chromatographic and capillary electrophoretic determination of microcystins

Trojanowicz

2010

J of Separation Science

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Microcystins are cyclic peptide hepatotoxins that are produced during blooming of cyanobacteria. In recent two decades they gained a great interest because of the related ecological and public health risks. Over 70 different analogs of microcystins have been isolated from natural blooms and laboratory cultures of cyanobacteria. Because they are inhibitors of protein phosphatases they act as tumor promoters, but on the other hand, inhibitive enzymatic and ELISA methods may be employed for their screening or quantitative determination. Much larger analytical potential for this purpose have, however high-performance separation chromatographic and electromigration techniques. Based on about 70 references from original journal papers these methods are reviewed together with presentation of their preconcentration methods and clean-up procedures for analyzing environmental samples. Some attention is also focused on increasing application of LC/MS methods, and comparison of separation techniques with immunochemical or enzymatic methods.

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Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography

Cited by 42 publications

References 29 publications

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

Current Techniques for Detecting and Monitoring Algal Toxins and Causative Harmful Algal Blooms

Chromatographic and capillary electrophoretic determination of microcystins

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