Electrochemical detection of peptides

Warner, Anne M.; Weber, Stephen G.

doi:10.1021/ac00198a015

Cited by 52 publications

(22 citation statements)

References 58 publications

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“…Tripeptides (1,2) have an E 1a2 of about 0.65 V and that of tetra-and longer peptides (3±11) have E 1a2 of about 0.45 V, except for peptide 8 which has a proline as its 4th amino acid. Its E 1a2 is closer to that of a tripeptide, re¯ecting the participation of the amide carbonyl (between R and P, the third and fourth amino acids from the amine terminus) as the fourth donor.…”

Section: Electrochemical Propertiesmentioning

confidence: 99%

Effect of Peptide Primary Sequence on Biuret Complex Formation and Properties

1999

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Visible spectroscopy and the rotating ring-disk electrode were used to study Cu(II)-peptide complexation of 21 peptides including Nacylated and N-pyroglutamyl peptides. All of the investigated peptides formed a complex with copper ion, and those complexes were electroactive. Peptides containing proline near the amine terminus, and N-acylated peptides, had lower rate constants for the formation of the electroactive complexes. Reaction rates were not signi®cantly decreased in peptides with a pyroglutamyl amine terminus. These complexes demonstrated very low oxidation potentials and some unusual spectroscopic properties.

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Section: Electrochemical Propertiesmentioning

confidence: 99%

Effect of Peptide Primary Sequence on Biuret Complex Formation and Properties

1999

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“…The Cu-based approach works best for tripeptides and longer peptides [48]. Although detection limits are a little poorer, dipeptides can be determined either with post-column reaction [49][50][51] or through the use of a modified electrode.…”

Section: Post-column Reactionsmentioning

confidence: 99%

Techniques for neuropeptide determination

Sandberg

Weber

2003

TrAC Trends in Analytical Chemistry

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Because immunoassay responds to epitopes, and many molecules share the same peptide epitope, it is very difficult to obtain an accurate understanding of peptides, their creation and hydrolysis, in biological systems. Separate-and-detect approaches have merit in that the many active peptides and inactive fragments of a particular system can be separately determined. This review discusses the separation, by chromatography and capillary electrophoresis, and detection, by absorbance, fluorescence, electrochemistry, and immunoassay techniques. When separation pre-concentration is accompanied by laser-induced fluorescence or biuret-based electrochemical detection, nM-pM detection limits are obtained.

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“…This has the drawback that most of these reagents react with all primary amines; therefore, amino acids, which are generally present in higher concentrations, interfere with the analysis. Weber's group at the University of Pittsburgh developed a selective method for the electrochemical detection of peptides based on the biuret reaction [29]. This reaction is specific for the amide bond of the peptide and shows a great deal of selectivity over amino acids.…”

Section: Peptidesmentioning

confidence: 99%

Pharmaceutical and biomedical applications of capillary electrophoresis/electrochemistry

Lunte

O’Shea

1994

Electrophoresis

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The use of capillary electrophoresis/electrochemistry (CEEC) for the analysis of microdialysis samples obtained for pharmacokinetic and neurochemical studies is described, as well as the development of new types of electrodes and waveforms which increase the selectivity of this technique for specific classes of analytes. CEEC with a carbon fiber electrode was employed for the analysis of microdialysis samples. Microdialysis is an in vivo sampling technique that yields very small samples for analysis (less than 1 microL). Therefore, capillary electrophoresis, with its small volume requirements, is an excellent choice for the analytical method. CEEC was used to study the pharmacokinetics of L-dopa and the release of aspartate and glutamate following a high K+ infusion in the brain. Several modified electrodes which increase the applicability of CEEC in pharmaceutical and biomedical analysis are described. One of these is a gold/mercury electrode which is highly selective for thiols and was used for the determination of glutathione in a rat brain. An alternative method for the detection of thiols employed a chemically modified electrode containing cobalt phthalocyanine. In this case, an electrocatalyst reduces the overpotential of thiols at the carbon electrode and makes it possible to detect them at a much lower and more selective oxidation potential. This electrode was used for the detection of cysteine in urine. The development of pulsed amperometric detection for capillary electrophoresis is also described and is demonstrated by the detection of glucose in blood. Lastly, a method for the detection of peptides based on the formation of a copper complex and detection at a carbon fiber electrode is discussed.

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Electrochemical detection of peptides

Cited by 52 publications

References 58 publications

Effect of Peptide Primary Sequence on Biuret Complex Formation and Properties

Effect of Peptide Primary Sequence on Biuret Complex Formation and Properties

Techniques for neuropeptide determination

Pharmaceutical and biomedical applications of capillary electrophoresis/electrochemistry

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