Electrochemical Detection of Peroxynitrite Using a Biosensor Based on a Conducting Polymer−Manganese Ion Complex

Abstract

A peroxynitrite (ONOO(-)) biosensor has been developed through the preparation of a new manganese-[poly-2,5-di-(2-thienyl)-1H-pyrrole)-1-(p-benzoic acid)] (Mn-pDPB) complex. DPB monomer was first synthesized and polymerized for the purpose of providing a polymer backbone for complex formation with Mn(2+) ion. The Mn-pDPB complex was characterized via Magnetomotive Force (MMF) simulation, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry. The complex selectively enhanced the reduction process of ONOO(-) which was used as the analytical signal for chronoamperometric detection. A polyethyleneimmine (PEI) layer was coated on the complex surface to increase selectivity and stability. The chronoamperometric calibration plot showed the hydrodynamic range of 2.0 × 10(-8)-5.0 × 10(-7) M. The detection limit was determined to be 1.9 (±0.2) × 10(-9) M based on S/N = 3. The microbiosensor, fabricated on a 100 μm diameter Pt tip, was applied in a real rat plasma sample for the detection of spiked concentrations of ONOO(-). The reliability and long-term stability of the microbiosensor was also examined with YPEN-1 cells in vitro, and the results shown were promising.

Electrochemical detection of peroxynitrite using hemin–PEDOT functionalized boron-doped diamond microelectrode

“…To enhance selectivity, the hemin-PEDOT-modified microelectrode was covered with a polyethyleneimine (PEI) membrane by dip coating three times in a 1.5% PEI aqueous solution (Sigma-Aldrich). PEI is a polymeric amine with high charge density that screens against cation permeation and also prevents fouling [24]. …”

Meat Freshness: Peroxynitrite’s Oxidative Role, Its Natural Scavengers, and New Measuring Tools

“…Moreover, an increase in selectivity was reported by using an outer layer of polyethyleneimine (PEI). The interferents tested included several electroactive species and PON decomposition molecules (133). The selectivity was reported as satisfactory, allowing these Mn-pDPB-PEI μEs to be employed for the PON determination in vitro on glioma tumor cells.…”

A simple degradation method for sulfur mustard at ambient conditions using nickelphthalocyanine incorporated polypyrrole modified electrode

“…Conducting polymers have been extensively used since last four decades as novel materials for potential applications in various electrochemical devices including actuators (Shoa et al 2010), chemical and biosensors (Koh et al 2010), capacitors (Lee et al 2010) and electrocatalyst (Qu et al 2010). The most commonly applied polymers for sensing applications have been based on polypyrrole (pPy), polyaniline (PANI), polythiophene (PTP) and their derivatives (Pirsa and Alizade 2010).…”