Electrochemical detection of proteins in high-performance liquid chromatography using on-line, postcolumn photolysis

Dou, L.; Holmberg, Anders; Krull, Ira S.

doi:10.1016/0003-2697(91)90407-k

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…2a ). Examples of these include high-performance liquid chromatography with optical detection 38 , with tandem mass spectrometry 39 or with electrochemical detection 40 ; capillary zone electrophoresis coupled to either optical detection or mass spectrometry 41 , 42 ; micellar electrokinetic chromatography 43 ; and capillary gel electrophoresis 44 . Among these, mass spectroscopy-based strategies offer high specificity towards insulin and its major degradation products and analogues, along with very low limits of detection.…”

Section: Current Insulin Measurement Approachesmentioning

confidence: 99%

Insulin detection in diabetes mellitus: challenges and new prospects

et al. 2023

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Tremendous progress has been made towards achieving tight glycaemic control in individuals with diabetes mellitus through the use of frequent or continuous glucose measurements. However, in patients who require insulin, accurate dosing must consider multiple factors that affect insulin sensitivity and modulate insulin bolus needs. Accordingly, an urgent need exists for frequent and real-time insulin measurements to closely track the dynamic blood concentration of insulin during insulin therapy and guide optimal insulin dosing. Nevertheless, traditional centralized insulin testing cannot offer timely measurements, which are essential to achieving this goal. This Perspective discusses the advances and challenges in moving insulin assays from traditional laboratory-based assays to frequent and continuous measurements in decentralized (point-of-care and home) settings. Technologies that hold promise for insulin testing using disposable test strips, mobile systems and wearable real-time insulin-sensing devices are discussed. We also consider future prospects for continuous insulin monitoring and for fully integrated multisensor-guided closed-loop artificial pancreas systems.

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Section: Current Insulin Measurement Approachesmentioning

confidence: 99%

Insulin detection in diabetes mellitus: challenges and new prospects

et al. 2023

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“…Todavia, no caso da fenilanalina, a fotodecomposição pós--coluna facilita a detecção eletroquímica, vez que leva à formação de 4 espécies eletroativas: L-dopa e os três isômeros da tirosina (o-, m-e p-). 84,85 Kaufman e colaboradores 83 observaram grande aceleração na fotodecomposição da fenilalanina pelo emprego do TiO 2 imobilizado na parede interna do reator pós-coluna de Teflon, mesmo para um curto período de residência da amostra. Em seguida Kissinger e colaboradores 86 exploraram o uso de um reator fotocatalítico em bobina para a determinação por detecção eletroquímica de 3-nitrotirosina, um possível marcador de peroxinitrito, espécie relacionada com o óxido nítrico e sua citotoxicidade.…”

Section: Emprego De Reator Fotocatalítico Para Derivatização Pré-ou Punclassified

FOTOCATÁLISE MEDIADA POR TiO2 NO ESTADO NANOPARTICULADO: REVISÃO DA REATIVIDADE PELO CONCEITO DE TRAPS E ALGUMAS APLICAÇÕES EM QUÍMICA ANALÍTICA

Baccaro¹,

Cordon²,

Nishimura³

et al. 2019

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TiO 2-MEDIATED PHOTOCATALYSIS AT THE NANOPARTICULATE STATE: REVIEW OF THE REACTIVITY BY THE TRAPPING CONCEPT AND SOME APPLICATIONS IN ANALYTICAL CHEMISTRY. TiO 2 has been used as a white pigment and studied since the first decades of the XX century for the undesirable chalking of outdoor paintings exposed to sunlight. Since the first report of Honda and Fujishima (1972) of the water splitting by the harvesting of sunlight on a n-type single crystal TiO 2 electrode, the engagement of researchers with the field has grown steadily. TiO 2-photocatalysis has joined the group of advanced oxidation processes for potential new water and wastewater treatments but, despite of its high chemical and photochemical stability and the great oxidizing power of its photogenerated holes, photons in UVA spectral region are needed to accomplish the high band gap, limiting the yield of sun-driven environmental applications. Nevertheless, this shortcoming has not restricted benchtop research and small and medium scale applications also relying on artificial UV sources, especially nowadays that highly-efficient UVA-LEDs are available. Herein, we review the fundamental aspects and the practical attributions of the trapping model in the charge transfer kinetics of photogenerated holes in nanoparticulate TiO 2. Regarding to new practical uses, we focus our attention on some ingenious applications of this photocatalyst in the field of analytical chemistry, covering also subjects never reviewed before.

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“…Proteins containing fluorescent moieties, e.g., tryptophan, can be detected by fluorescence detection. Dou et al (1991) used electrochemical oxidation for the detection of proteins (e.g., albumin and insulin) after photolysis of the protein at 254 nm. The photolysis includes the cleavage of disulfide bonds, resulting in the formation of oxidizable sulfhydryl species and cleavage of amino acid side chains.…”

Section: Detectionmentioning

confidence: 99%