Electrochemical detection of specific interactions between apolipoprotein E isoforms and DNA sequences related to Alzheimer’s disease

Lima, Dhésmon; Hacke, Ana Carolina Mendes; Inaba, Juliana; Pessôa, Christiana Andrade; Kerman, Kağan

doi:10.1016/j.bioelechem.2019.107447

Cited by 30 publications

(25 citation statements)

References 57 publications

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“…In recent years, researchers have also proved that SIRT1 gene regulates obesity and lipid metabolism, for example, Thaddeus et al 55 pointed out that SIRT1 gene may affect obesity by regulating fatty acid oxidation in the liver, influencing obesity-induced inflammation in macrophages, sensing nutrient availability in the hypothalamus, as well as modulating the activity of the circadian clock in metabolic tissues. In addition, the interaction between APOE and SIRT1 may also have an impact on individual’s obesity and cognition 56 , for instance, Jesus Campagna et al 57 found that the expression of APOE E4 would decrease the level of SIRT1 in serum and brain tissue of AD patients as compared to normal controls; Veena et al 58 found that APOE E4 could reduce the expression of SIRT1 expression and thus played a neuroprotective role; what’s more, María Teresa Flores-Dorantes et al 59 also found that SIRT1 gene could be affected by APOE gene, and then regulated obesity. Therefore, we hypothesized that obesity in patients with schizophrenia may also be influenced by APOE E4 and SIRT1 genes, but these conclusions need to be verified in future studies.…”

Section: Discussionmentioning

confidence: 99%

APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients

Liu

et al. 2021

Sci Rep

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Obesity is a critical issue in patients with schizophrenia, which is considered to be brought about by both environmental and genetic factors. Apolipoprotein E (APOE) gene polymorphisms might be involved in the pathogenesis of schizophrenia, however, the effect of APOE gene polymorphism on obesity has never been investigated in Chinese aging with schizophrenia. This cross-sectional study was to investigate the effect of obesity on cognitive and psychiatric symptoms in elderly participants with schizophrenia. At the same time, we also discussed the inner link between APOE E4 and obesity. 301 elderly participants with schizophrenia and 156 normal controls were included in the study. Their cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), psychiatric symptoms were assessed using the Positive and Negative Syndrome Scale (PANSS), and APOE gene polymorphism was determined by polymerase chain reaction (PCR). The prevalence of obesity in elderly schizophrenic patients and healthy controls accounted for 15.9% (48/301) and 10.3% (16/156), respectively, with no statistically significant difference. By using stepwise linear regression analysis, we found that elevated fasting blood glucose, hypertension, and hyperlipidemia were risk factors for obesity in elderly schizophrenic patients. Although there was no direct correlation between APOE E4 and obesity in patients with schizophrenia, it was significantly correlated with hyperlipemia(r = − 0.154, p = 0.008), suggesting that APOE E4 may induce obesity in elderly patients with schizophrenia through hyperlipemia, However, the above conclusions do not apply to the normal elderly. What’s more, we did not find a link between obesity and cognitive function or mental symptoms for both patients with schizophrenia and normal controls. APOE E4 is associated with hyperlipidemia in elderly schizophrenic patients, which may be a risk factor for obesity, however, the above conclusion does not apply to the normal elderly.

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Section: Discussionmentioning

confidence: 99%

APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients

Liu

et al. 2021

Sci Rep

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“…Cyclic voltammetry (CV) is widely used for determining the interactions of small biologically active molecules with DNA [ 2 , 3 , 24 , 26 , 42 , 59 , 61 , 79 , 80 , 81 , 82 ]. During the measurement, a linearly changing potential is applied to the working electrode.…”

Section: Electrochemical Methods Appliedmentioning

confidence: 99%

“…The stationary working electrode is composed of various types of electrode materials. The most common are glassy carbon electrodes (GCEs) [ 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ], pencil graphite electrodes (PGEs) [ 50 , 51 , 52 , 53 , 54 , 55 , 56 ], carbon paste electrodes (CPEs) [ 57 , 58 ], hanging mercury drop electrodes (HMDEs) [ 59 ], platinum electrodes [ 60 ], and gold electrodes [ 61 , 62 ]. These electrodes can be used either in the bare form [ 44 , 54 , 63 ] or modified with a layer capable of increasing their sensitivity [ 50 , 51 , 52 , 53 , 55 , 56 ].…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

“…Consequently, the impact of the subsequent portions (increasing the DNA concentration) on the redox processes of the examined system is determined [ 24 , 26 , 44 , 46 , 59 , 63 ]. An alternative method is the modification of the electrode, which involves immobilizing one of the analyzed system elements (studied compound with biological activity [ 64 ] or DNA [ 41 , 42 , 57 , 61 , 62 , 63 , 65 , 66 ]) on the surface of the working electrode. The last-mentioned approach is often used to obtain DNA biosensor systems for monitoring drug interactions.…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

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What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

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The interactions of compounds with DNA have been studied since the recognition of the role of nucleic acid in organisms. The design of molecules which specifically interact with DNA sequences allows for the control of the gene expression. Determining the type and strength of such interaction is an indispensable element of pharmaceutical studies. Cognition of the therapeutic action mechanisms is particularly important for designing new drugs. Owing to their sensitivity, simplicity, and low costs, electrochemical methods are increasingly used for this type of research. Compared to other techniques, they require a small number of samples and are characterized by a high reliability. These methods can provide information about the type of interaction and the binding strength, as well as the damage caused by biologically active molecules targeting the cellular DNA. This review paper summarizes the various electrochemical approaches used for the study of the interactions between pharmaceuticals and DNA. The main focus is on the papers from the last decade, with particular attention on the voltammetric techniques. The most preferred experimental approaches, the electrode materials and the new methods of modification are presented. The data on the detection ranges, the binding modes and the binding constant values of pharmaceuticals are summarized. Both the importance of the presented research and the importance of future prospects are discussed.

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“…Electrochemical impedance spectroscopy (EIS) is a label-free detection method which has been applied for various different types of targets ranging from entire bacteria detection with immobilized antibodies, over nucleic acid targets to proteins and small molecules [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. We have previously developed in the Bachmann group EIS biosensors for the detection of the mecA gene and genomic DNA of methicillin-resistant S. aureus (MRSA), the New Delhi Metallo-beta-lactamase (NDM) carbapenem-resistance gene and for bacterial 16S ribosomal RNA for bacterial species identification [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Schulze

Wilson

Cara

et al. 2021

Sensors

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Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

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Electrochemical detection of specific interactions between apolipoprotein E isoforms and DNA sequences related to Alzheimer’s disease

Cited by 30 publications

References 57 publications

APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients

APOE E4 is associated with hyperlipidemia and obesity in elderly schizophrenic patients

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

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