Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Kang, Yong-Koo; Feng, Keqin; Chen, Jiwei; Jiang, Jian‐Hui; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.bioelechem.2008.04.024

Cited by 94 publications

(34 citation statements)

References 49 publications

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“…For electrochemical detection of the analyte-aptamer interaction it is also advantageous to immobilize aptamers onto composite films with desired properties, that provide good charge transfer between the sensing layer and the support. For example, Kang et al [66] used for this purpose nanogold-chitosan composite film. The film was formed at the surface of glassy carbon electrode.…”

Section: Immobilization Of Aptamers On Solid Supportsmentioning

confidence: 98%

Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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This article reviews recent achievements in developing aptamer-based electrochemical biosensors (electrochemical aptasensors). Aptamers are single stranded DNA or RNA molecules with high specificity to various ligands. Their specificity is comparable and in certain cases even higher than those of antibodies. In contrast to antibodies, aptamers are prepared by an in vitro selection procedure developed simultaneously in the early 1990s by L. Gold and A. Ellington. Due to their stability and the possibility of chemical modification aptamers can be immobilized on various supports and serve as artificial receptors in biosensors. The first aptasensors developed in the second half of 1990s were based on optical detection. However, in early 2000 substantial interest arose to the development of electrochemical aptasensors. It has been shown that due to their simplicity and fast response they represent an excellent tool in practical applications. The main focus of this review is to discuss the configuration of aptamers and electrochemical methods for detecting aptamers -analyte interactions. We will also provide a brief history of aptamer development, along with molecular structure and methods of aptamer engineering. Methods for immobilizing aptamers onto a solid support are also discussed.

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Section: Immobilization Of Aptamers On Solid Supportsmentioning

confidence: 98%

Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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“…The electrochemical active marker used was methylene blue (MB) directly intercalated in the probing aptamer. The sensor linear response for thrombin was in the range 1-60 nM with a 0.5 nM detection limit [66].…”

Section: Sandwich-type G4 Electrochemical Aptasensormentioning

confidence: 97%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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“…Although the majority of sandwich-type G-quadruplex electrochemical aptasensors reported in the literature was using the immobilisation of the first aptamer via thiol Apart from the aptamer-analyte-aptamer sandwich approach, other design strategies were also employed, consisting in either attaching the analyte to the surface via an antibody combined with a labelled G-quadruplex aptamer for detection (antibodyanalyte-aptamer sandwich, Scheme 3B) [65], or attaching the analyte to the surface via a G-quadruplex aptamer, the detection being performed with a redox labelled antibody (aptamer-analyte-antibody sandwich, Scheme 3C) [61].…”

Section: Sandwich-type G-quadruplex Electrochemical Aptasensorsmentioning

confidence: 99%

Redox behaviour of G-quadruplexes

Chiorcea-Paquim

Oliveira-Brett

2014

Electrochimica Acta

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Please cite this article as: A.-M. Chiorcea-Paquim, A.M. OliveiraBrett, Redox behaviour of G-quadruplexes, Electrochimica Acta (2013), http://dx.doi.org/10.1016/j.electacta. 2013.07.150 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 45A c c e p t e d M a n u s c r i p t Redox behaviour of G-quadruplexes

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Electrochemical detection of thrombin by sandwich approach using antibody and aptamer

Cited by 94 publications

References 49 publications

Electrochemical Aptasensors – Recent Achievements and Perspectives

Electrochemical Aptasensors – Recent Achievements and Perspectives

Guanine Quadruplex Electrochemical Aptasensors

Redox behaviour of G-quadruplexes

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