A well-known textile dye, methylene blue was determined using glassy carbon electrode (GCE), modified with green and chemically synthesized silver nanoparticles (AgNPs) doped in functionalized multiwalled carbon nanotubes (fMWCNT). The green and chemical methods were used to synthesize silver (Ag) nanoparticles, which were subsequently doped with multi-walled carbon nanotubes (MWCNT) forming MWCNT/Agchm and MWCNT/Aggrn nanocomposite. Comprehensive characterization of the nanomaterials was carried out using energy-dispersive X-ray spectroscopy (EDX) detector-equipped scanning electron microscopy (EM), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV), and X-ray diffraction (XRD). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and square wave voltammetry (SWV) were employed for the investigation of the electrochemical properties and behaviour of MWCNT, Agchm, Aggrn, Agchm/MWCNT, and Aggrn/MWCNT electrodes, and higher electron transport capabilities and improved electrochemical activity towards methylene blue (MB) on Agchm/MWCNT electrode were demonstrated by the results. With the linear range of .927 pM to 15.652 pM, SWV was used to detect MB. For Agchm/MWCNT, a low limit of detection (LOD) of 4.684 and limit of quantification (LOQ) 14.194 pM, and for Aggrn/MWCNT 2.935 and 8.895 pM, respectively. In real sample analysis, the recovery percentage for Agchm/MWCNT ranged from 90 to 98% (n =3), and Aggrn/MWCNT showed a recovery percentage ranging from 97 to 103% (n = 3). Both electrodes’ remarkable recovery rate attest to their dependability and sensitivity in MB detection.