Electrochemical determination of lead and glutathione in a plant cell culture

Vacek, Jan; Petřek, Jiřı́; Kizek, René; Havel, Ladislav; Klejdus, Bořivoj; Trnková, Libuše; Jelen, František

doi:10.1016/j.bioelechem.2003.08.007

Cited by 63 publications

(39 citation statements)

References 24 publications

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“…clone PE 14. The heavy metals were added to cultivation medium in form of EDTA chelates (Cd-EDTA and Pb-EDTA), because if we used metals as salts (nitrates, sulphates, acetates), we observed precipitation [15]. Therefore, the concentration of free metal was dubious [44][45][46].…”

Section: Changes In Morphology Of Somatic Embryos Under Heavy Metals mentioning

confidence: 99%

“…Primarily, we were working on the assumptions of a recently published paper, where GSH had been adsorbed on the surface of working electrode (HMDE) for 120 s [15]. Thus, we did the same and adsorbed GSH (100 µM) gave five following signals Co1, RS 2 Co, Cat1, Cat2 and Cat3.…”

Section: Electrochemical Detection Of Glutathione -Use Of the Brdickamentioning

confidence: 99%

“…PCs have a basic formula (γ-Glu-Cys)n-Gly (n = 2 to 11) and form M-PC complexes with the heavy metals (M), in which the metal is bound via the cysteine unit SH group [1,6,7]. A very wide range of analytical techniques, including both chromatographic coupled with different detectors and stationary ones, such as electrochemical methods, have been utilized for determination of thiols in samples of interest [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…Recently we were concerned with determination of glutathione and phytochelatins by different electrochemical techniques [15,17,[47][48][49][50][51].…”

Section: Electrochemical Detection Of Glutathione -Use Of the Brdickamentioning

confidence: 99%

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Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Šupálková

Petřek

Baloun

et al. 2007

Sensors

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Abstract:The main aim of this work was to use multi-instrumental analytical apparatus to investigate the effects of treatment with cadmium(II) and/or lead(II) ions (50, 250 and 500 µM) for twelve days on early somatic spruce embryos (ESEs). Primarily we used image analysis for estimation of growth and a fluorimetric sensor for enzymatic detection of viability of the treated ESEs. It follows from the obtained results that Cd caused higher toxicity to ESEs than Pb. Besides this fundamental finding, we observed that ESEs grew and developed better in the presence of 500 µM of the metal ions than in the presence of 250 µM. Based on the results obtained using nuclear magnetic resonance this phenomenon was related to an increase of the area of ESE clusters by intensive uptake of water from the cultivation medium, due to dilution of the heavy metal concentration inside the cluster. In addition we studied the glutathione content in treated ESEs by the adsorptive transfer stripping technique coupled with the differential pulse voltammetry Brdicka reaction. GSH contents increased up to 148 ng/mg (clone 2/32) and 158 ng/mg (clone PE 14) after twelve Sensors 2007, 7 744 day long treatment with Cd-EDTA ions. The GSH content was about 150 and 160 % higher in comparison with the ESEs treated with Pb-EDTA ions, respectively. The difference between GSH contents determined in ESEs treated with Pb-EDTA and Cd-EDTA ions correlates with the higher toxicity of cadmium(II) ions.

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Section: Changes In Morphology Of Somatic Embryos Under Heavy Metals mentioning

confidence: 99%

Section: Electrochemical Detection Of Glutathione -Use Of the Brdickamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Recently we were concerned with determination of glutathione and phytochelatins by different electrochemical techniques [15,17,[47][48][49][50][51].…”

Section: Electrochemical Detection Of Glutathione -Use Of the Brdickamentioning

confidence: 99%

See 2 more Smart Citations

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Šupálková

Petřek

Baloun

et al. 2007

Sensors

Self Cite

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“…GSH gave a signal designated as P GSH at a potential of -0.44 V, GSSG a signal designated as P GSSG at -0.69 V. As for GSH, both reductive and oxidative signals are evident. The reductive signal corresponds to a reduction of the GS-Hg complex occurring on the surface of HMDE Vacek et al, 2004b). Particularly, we assume that thiols are firstly adsorbed on the working electrode surface and then a complex of thiol with mercury (GS-Hg) is formed.…”

Section: Cyclic Voltammograms Of Thiolsmentioning

confidence: 99%

A sensor for investigating the interaction between biologically important heavy metals and glutathione

Húska¹,

Zítka²,

Adam³

et al. 2007

Czech J. Anim. Sci.

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Glutathione (GSH) is a water-soluble tripeptide playing a crucial role in the number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in an oxidized (GSSG) and reduced (GSH) state. The aim of this paper was to use electrochemical methods to determine glutathiones and to investigate interactions of GSH and cadmium, zinc, copper and nickel ions or feed additive for animal nutrition. It clearly follows from the results that GSH and GSSG can be distinguished by cyclic voltammetry whereas the level of their current response is proportional to their concentration. Moreover, we studied these interactions by cyclic voltammetry. Marked changes in the GSH signal were observed and discussed. The highest decrease in a GSH reductive signal is caused by cadmium ions followed by zinc, nickel and copper ones. We also observed that the reductive GSH signal gradually decreased (about 9%) with the increasing concentration of feed additive used in our experiments. In conclusion, cyclic voltammetry offers a tool for studying interactions of thiols with different substances such as heavy metals and/or feed additives. Keywords:

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Study of Metallothionein Modified Electrode Surface Behavior in the Presence of Heavy Metal Ions-Biosensor

et al. 2005

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An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behavior of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -forming of the surface assembled monolayer -was probably reached in about 240 s for 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analyzed in the presence of the basic electrolyte -0.5 M NaCl (pH 6.4), were 250 fmol and 350 fmol in 5 mL drop, respectively. In addition, we applied the biosensor to analyze heavy metals in human body liquids (human blood serum and human urine) and to compare with differential pulse anodic stripping voltammetry.

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Electrochemical determination of lead and glutathione in a plant cell culture

Cited by 63 publications

References 24 publications

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

A sensor for investigating the interaction between biologically important heavy metals and glutathione

Study of Metallothionein Modified Electrode Surface Behavior in the Presence of Heavy Metal Ions-Biosensor

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