Electrochemical Determination of <scp>L</scp>‐Dopa in <i>Mucuna pruriens</i> Seeds, Leaves and Commercial Siddha Product Using Gold Modified Pencil Graphite Electrode

This paper describes the development and utilization of a new nanocomposite consisting of graphene quantum dots, magnetic nanoparticles and carboxylated multiwalled carbon nanotubes for glassy carbon electrode surface modification. The graphene quantum dots were synthesized via pyrolysis of citric acid. The nanocomposite was characterised by transmission electron microscopy (TEM), X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The modified electrode was used for electrochemical determination of L-DOPA in some agricultural samples. Using differential pulse voltammetry, the prepared sensor showed good sensitivity and selectivity with low overpotential for the determination of L-DOPA in the range from 3.0 to 400 μmol L -1 , with a detection limit of 14.3 nmol L -1 . Electrochemical studies suggested that the modified electrode provided a synergistic augmentation on the voltammetric behaviour of electrochemical oxidation of L-DOPA. The amount of L-DOPA in sunflower seed, sesame seed, pumpkin seed and fava bean seed were evaluated with the proposed sensor.

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“…As can be seen from Fig. 43 In other words, the number of transferred protons and electrons are equal. 1d (B)) was found to be less than 10 nm.…”

Section: Characterisation Of Fe 3 O 4 @Gqdsmentioning

confidence: 83%

“…For diffusion controlled process, the peak potential, E p , could be presented by the following equations: 43 …”

Section: Scan Rate Effectmentioning

confidence: 99%

Graphene quantum dots decorated with Fe₃O₄ nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

2016

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“…Nanomaterials may be immobilized or prepared on GE surfaces via various techniques, including chemical vapor deposition [11], electrochemical deposition from a metal precursor [12][13][14][15], or electro chemical deposition from polymer attached NPs [16,17]. Chemical vapor deposition and electrochemical deposition techniques are fast processes and, therefore, produce poorly reproducible results.…”

Section: Methods For Immobilizationmentioning

confidence: 99%

Recent developments on the modification of graphite electrodes with nanoparticles

Kakhki

2016

Russ J Appl Chem

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The surface structure of common graphite electrodes are suitable for electrochemical detection of various analytes due to their favorable properties such as good conductivity and resistance to environmental and chemical hazards. Also this material is cheap and available. Modifying the surface of electrode improves their ability for various determinations. Modifying graphite electrodes with nanoparticles has attracted lots of attention due to their unique characteristics. In this article we review applications of modified graphite electrodes with nanomaterials.

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“…Electrochemical approaches have attracted much attention so far, since electrochemistry possesses a couple of merits including considerably high sensitivity and specificity. Recently, Kalachar et al presented a voltammetric method for the direct determination of L‐DOPA in M. pruriens . They first prepared a nanogold deposition graphite electrode by constant potential technique.…”

Section: Introductionmentioning

confidence: 99%

Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine inMucuna pruriens

Chen

Yang

et al. 2013

J. Sep. Science

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L-3,4-dihydroxyphenylalanine (L-DOPA) is a well-recognized therapeutic compound to Parkinson's disease. Tyrosine is a precursor for the biosynthesis of L-DOPA, both of which are widely found in traditional medicinal material, Mucuna pruriens. In this paper, we described a validated novel analytical method based on microchip capillary electrophoresis with pulsed electrochemical detection for the simultaneous measurement of L-DOPA and tyrosine in M. pruriens. This protocol adopted end-channel amperometric detection using platinum disk electrode on a homemade glass/polydimethylsiloxane electrophoresis microchip. The background buffer consisted of 10 mM borate (pH 9.5) and 0.02 mM cetyltrimethylammonium bromide, which can produce an effective resolution for the two analytes. In the optimal condition, sufficient electrophoretic separation and sensitive detection for the target analytes can be realized within 60 s. Both tyrosine and L-DOPA yielded linear response in the concentration range of 5.0-400 μM (R(2) > 0.99), and the LOD were 0.79 and 1.1 μM, respectively. The accuracy and precision of the established method were favorable. The present method shows several merits such as facile apparatus, high speed, low cost and minimal pollution, and provides a means for the pharmacologically active ingredients assay in M. pruriens.

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Electrochemical Determination of L‐Dopa in Mucuna pruriens Seeds, Leaves and Commercial Siddha Product Using Gold Modified Pencil Graphite Electrode

Cited by 28 publications

References 36 publications

Graphene quantum dots decorated with Fe₃O₄ nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

Graphene quantum dots decorated with Fe₃O₄ nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

Recent developments on the modification of graphite electrodes with nanoparticles

Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine inMucuna pruriens

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Electrochemical Determination of L‐Dopa in Mucuna pruriens Seeds, Leaves and Commercial Siddha Product Using Gold Modified Pencil Graphite Electrode

Cited by 28 publications

References 36 publications

Graphene quantum dots decorated with Fe3O4 nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

Graphene quantum dots decorated with Fe3O4 nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

Recent developments on the modification of graphite electrodes with nanoparticles

Development of a microchip-pulsed electrochemical method for rapid determination of L-DOPA and tyrosine inMucuna pruriens

Contact Info

Product

Resources

About

Graphene quantum dots decorated with Fe₃O₄ nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products

Graphene quantum dots decorated with Fe₃O₄ nanoparticles/functionalized multiwalled carbon nanotubes as a new sensing platform for electrochemical determination of l-DOPA in agricultural products