Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor

Tanaka, Tsuyoshi; Shimoda, Mari; Shionoiri, Nozomi; Hosokawa, Masahito; Taguchi, Tomoyuki; Wake, Hitoshi; Matsunaga, Tadashi

doi:10.1016/j.jbiosc.2013.04.013

Cited by 21 publications

(14 citation statements)

References 41 publications

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“…Drastic changes were observed in the inner content and cell walls. Tanaka et al (2013) detected pronounced lipid peroxidation in the cell membranes of disinfected bacteria during electrolysis with Pt anode in seawater. Therefore, a comprehensive study is still in great demand to explore the subcellular inactivation mechanisms of electrochemical disinfection with BDD anode.…”

Section: Introductionmentioning

confidence: 98%

Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes

Long

Wang

2015

Water Research

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Section: Introductionmentioning

confidence: 98%

Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes

Long

Wang

2015

Water Research

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“…And it is known to inactivate a wide variety of microorganisms from bacteria to viruses and algae444546. One advantage of electrochemical disinfection is that the damage produced to the bacterial cells is more severe than that produced by pure chemical disinfection with chlorine47.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Effects of Micro-electrolysis-Photocatalysis on Water Treatment and Fish Performance in Saline Recirculating Aquaculture System

Wang

Gao

et al. 2017

Sci Rep

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A new physico-chemical process for TAN (total ammonia nitrogen) removal and disinfection is introduced in saline recirculating aquaculture system (RAS), in which the biofilter is replaced with an integrated electrolysis cell and an activated carbon filter. The electrolysis cell which is based on micro current electrolysis combined with UV-light was self-designed. After the fundamental research, a small pilot scale RAS was operated for 30 days to verify the technical feasibility. The system was stocked by 42 GIFT tilapia (Oreochromis niloticus) fish with the rearing density of 13 kg/m3. During the experiments, the TAN concentration remained below 1.0 mg/L. The nitrite concentration was lower than 0.2 mg/L and the nitrate concentration had increased continuously to 12.79 mg/L at the end. Furthermore, the concentration of residual chlorine in culture ponds remained below 0.3 mg/L, ORP maintained slight fluctuations in the range of 190~240 mV, and pH of the water showed the downtrend. Tilapia weight increased constantly to 339.3 ± 10 g. For disinfection, the active chlorine generated by electrochemical treatment caused Escherichia coli inactivation. Enzyme activity assay indicated that the activity of glutamate dehydrogenase, carbonic anhydrase and glutamic pyruvic transaminase increased within the normal range. The preliminary feasibility was verified by using this physico-chemical technology in the RAS.

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“…DPPP is nonfluorescent and selectively reacts with lipid hydroperoxide within the cell membrane due to its high lipophilic characteristic and is oxidized to fluorescent DPPP oxide (DPPPϭO) (76). Thus, the extent of incidence of lipid peroxidation within the cell membrane can be determined from the fluorescent value produced by oxidation of DPPP (77,78).…”

Section: Methodsmentioning

confidence: 99%

The Synergistic Bactericidal Mechanism of Simultaneous Treatment with a 222-Nanometer Krypton-Chlorine Excilamp and a 254-Nanometer Low-Pressure Mercury Lamp

Kang

2019

Appl Environ Microbiol

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The purpose of this study was to investigate the synergistic bactericidal effect of 222-nm KrCl excilamp and 254-nm low-pressure (LP) Hg lamp simultaneous treatment againstEscherichia coliO157:H7,Salmonella entericasubsp.entericaserovar Typhimurium, andListeria monocytogenesin tap water and to identify the synergistic bactericidal mechanism. Sterilized tap water inoculated with pathogens was treated individually or simultaneously with a 254-nm LP Hg lamp or 222-nm KrCl excilamp. Overall, for all pathogens, an additional reduction was found compared to the sum of the log unit reductions of the individual treatments resulting from synergy in the simultaneous treatment with both kinds of lamps. In order to identify the mechanism of this synergistic bactericidal action, the form and cause of membrane damage were analyzed. Total reactive oxygen species (ROS) and superoxide generation as well as the activity of ROS defense enzymes then were measured, and the overall mechanism was described as follows. When the 222-nm KrCl excilamp and the 254-nm LP Hg lamp were treated simultaneously, inactivation of ROS defense enzymes by the 222-nm KrCl excilamp induced additional ROS generation following exposure to 254-nm LP Hg lamp (synergistic) generation, resulting in synergistic lipid peroxidation in the cell membrane. As a result, there was a synergistic increase in cell membrane permeability leading to a synergistic bactericidal effect. This identification of the fundamental mechanism of the combined disinfection system of the 222-nm KrCl excilamp and 254-nm LP Hg lamp, which exhibited a synergistic bactericidal effect, can provide important baseline data for further related studies or industrial applications in the future.IMPORTANCEContamination of pathogenic microorganisms in water plays an important role in inducing outbreaks of food-borne illness by causing cross-contamination in foods. Thus, proper disinfection of water before use in food production is essential to prevent outbreaks of food-borne illness. As technologies capable of selecting UV radiation wavelengths (such as UV-LEDs and excilamps) have been developed, wavelength combination treatment with UV radiation, which is widely used in water disinfection systems, is actively being studied. In this regard, we have confirmed synergistic bactericidal effects in combination with 222-nm and 254-nm wavelengths and have identified mechanisms for this. This study clearly analyzed the mechanism of synergistic bactericidal effect by wavelength combination treatment, which has not been attempted in other studies. Therefore, it is also expected that these results will play an important role as baseline data for future research on, as well as industrial applications for, the disinfection strategy of effective wavelength combinations.

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Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor

Cited by 21 publications

References 41 publications

Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes

Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes

Synergistic Effects of Micro-electrolysis-Photocatalysis on Water Treatment and Fish Performance in Saline Recirculating Aquaculture System

The Synergistic Bactericidal Mechanism of Simultaneous Treatment with a 222-Nanometer Krypton-Chlorine Excilamp and a 254-Nanometer Low-Pressure Mercury Lamp

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