Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode

Nascimento, G.A.; Souza, Elaine V.M.; Campos-Ferreira, Danielly S.; Arruda, Mariana S.; Castelletti, Carlos Henrique Madeiros; Wanderley, Marcela Silvestre Outtes; Ekert, Marek Hf; Bruneska, Danyelly; Lima-Filho, José Luiz de

doi:10.1016/j.bios.2012.04.052

Cited by 44 publications

(15 citation statements)

References 36 publications

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“…Studies involving techniques of BPV diagnosis have attracted great interest due to the problems caused by the viral infection, such as dermatitis and cancer, resulting in significant economic impacts on livestock (Nascimento et al, 2012). Moreover, the results obtained through diagnostic techniques allow drawing a molecular epidemiology of the infections, which has prophylactic importance, since immunity is species-specific (Claus et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…However, polymerase chain reaction (PCR) has been shown to be a more sensitive method for the identification and characterization of BPV, by using degenerate primers followed by DNA purification, sequencing and sequence similarity analysis with computer programs and tools such as BLASTn (Borzacchiello et al, 2003;Ogawa et al, 2004;Jelínk and Tachezy, 2005;Monteiro et al, 2008;Leto et al, 2011;Carvalho et al, 2012;Nascimento et al, 2012;Zhu et al, 2012). Molecular diagnosis may also be performed by restriction fragment polymorphism length (RFLP), a technique that employs the fragmentation of the L1 gene by the use of restriction endonucleases (Carvalho et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

et al. 2014

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ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn. The blood diagnosis showed the presence of BPV-2 and the analysis of cutaneous papillomas showed the presence of BPV-4, a new First description of BPV-12 and putative type BAPV8 in Brazil putative virus type BAPV8, and BPV-12, revealing for the first time the presence of BPV-12 and the putative type BAPV8 in beef cattle in Brazil. The sequences were deposited in the GenBank. Histopathology revealed acanthosis, hyperkeratosis, and koilocytosis in all samples analyzed. The presence of BAPV8 and BPV-12 in Brazil emphasizes the ubiquitous dissemination of BPVs in the herds of Brazil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

et al. 2014

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“…BPV diagnosis usually includes a clinical examination, histopathology, and immunohistochemistry [20,21]. Polymerase chain reaction (PCR) has been used as a sensitive method for the identification and genotyping of BPV [22][23][24]. Specific primers have also been successfully employed mainly for BPV identification in blood [25,26].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

et al. 2017

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Objective: This study aims to provide a molecular and epidemiological characterization of bovine papillomavirus (BPV) infections in Iraq. Methods: The present study focuses on identifying BPV based on clinical and epidemiological manifestations, histopathological examinations, and polymerase chain reactions (PCR). Samples were collected from 163 animals suffering from cutaneous bovine papillomatosis, including 129 females (79.14%) with an age range of 16–40 months and 34 males (20.85%) with an age range of 17–29 months. Results: The incidence rate was significantly higher in females than in males. The most commonly affected sites were the teats and neck, though warts were found in other areas of the body. Histological sections were diagnosed as fibropapilloma. PCR results showed that 80.13% of the extracted papilloma DNA samples corresponded to the BPV-1 genotype. Furthermore, 7.94% of the samples showed a mixed infection of BPV-1 and BPV-13. While, 40.63% of the extracted DNA blood samples showed 2 DNA fragments corresponding to both genotypes BPV-1 and BPV-2. Conclusions: This study confirmed the presence of BPV-1, BPV-2, and BPV-13, which belong to the Deltapapillomavirus genera, for the first time in the DNA of Iraqi cattle. Understanding BPV diversity and epidemiology is of critical importance for starting prevention strategies.

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“…They can reduce the time and simplify the protocol of several assays, facilitating detection of specific nucleic acid sequence (Campos-Ferreira et al, 2013;Girousi and Kinigopoulou, 2010;Nascimento et al, 2012;Tang et al, 2009). DNA hybridization is based on the ability of ssDNA to recognize its complementary DNA sequence, forming the DNA duplex chain (hybrid) (Campos-Ferreira et al, 2013;Nascimento et al, 2012;Wang et al, 2008).…”

Section: Hybridization Detectionmentioning

confidence: 99%

Electrochemical DNA biosensor for the detection of human papillomavirus E6 gene inserted in recombinant plasmid

Campos-Ferreira

Souza

Nascimento

et al. 2016

Arabian Journal of Chemistry

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In the current study, we describe a novel, simple, inexpensive, sensitive, specific, stable and label-free electrochemical DNA biosensor used to identify a target gene cloned into a plasmid. The biosensor was designed with a 23-mer oligonucleotide of guanine-free, which was immobilized on the pencil graphite electrode (PGE) for E6 gene detection from human papillomavirus 16 type (HPV16). The E6 gene was used due to its clinical importance. The optimal probe concentration was obtained in 500 nM. The hybridization detection showed a good linearity in the range of 40-5,000 pg/lL with a detection limit of 16 pg/lL. The electrochemical method showed higher sensitivity and specificity when compared with the agarose gel electrophoresis assay. This technology could be postulated as a new and attractive alternative for cloning analysis in plasmids.ª 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.

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Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode

Cited by 44 publications

References 36 publications

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

Molecular Epidemiology of Bovine Papillomatosis and Identification of Three Genotypes in Central Iraq

Electrochemical DNA biosensor for the detection of human papillomavirus E6 gene inserted in recombinant plasmid

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