Electrochemical DNA biosensors: a review

Rafique, Bushra; Iqbal, Mudassir; Mehmood, Tahir; Shaheen, Muhammad

doi:10.1108/sr-08-2017-0156

Cited by 41 publications

(18 citation statements)

References 184 publications

(150 reference statements)

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“…While the detection of specific target pathogens and DNA fragments via hybridization has been made possible using conventional electrochemical or resonance frequency measurement circuits (Rafique et al , 2019; Singh et al , 2018; Vivek et al , 2019), design of electronics suitable for acquiring the signal of nanopore ion-channel electrochemical cells remains currently challenging because of the high-frequency components of the current waveform. This paper has reviewed the status of key technologies relevant to nanopore-based molecular measurement with an emphasis on an application to DNA sequencing.…”

Section: Summary and Discussionmentioning

confidence: 99%

Nanopore-based DNA sequencing sensors and CMOS readout approaches

Habibi

Dawji

Ghafar-Zadeh

et al. 2021

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Purpose Nanopore-based molecular sensing and measurement, specifically DNA sequencing, is advancing at a fast pace. Some embodiments have matured from coarse particle counters to enabling full human genome assembly. This evolution has been powered not only by improvements in the sensors themselves, but also in the assisting microelectronic CMOS readout circuitry closely interfaced to them. In this light, this paper aims to review established and emerging nanopore-based sensing modalities considered for DNA sequencing and CMOS microelectronic methods currently being used. Design/methodology/approach Readout and amplifier circuits, which are potentially appropriate for conditioning and conversion of nanopore signals for downstream processing, are studied. Furthermore, arrayed CMOS readout implementations are focused on and the relevant status of the nanopore sensor technology is reviewed as well. Findings Ion channel nanopore devices have unique properties compared with other electrochemical cells. Currently biological nanopores are the only variants reported which can be used for actual DNA sequencing. The translocation rate of DNA through such pores, the current range at which these cells operate on and the cell capacitance effect, all impose the necessity of using low-noise circuits in the process of signal detection. The requirement of using in-pixel low-noise circuits in turn tends to impose challenges in the implementation of large size arrays. Originality/value The study presents an overview on the readout circuits used for signal acquisition in electrochemical cell arrays and investigates the specific requirements necessary for implementation of nanopore-type electrochemical cell amplifiers and their associated readout electronics.

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Section: Summary and Discussionmentioning

confidence: 99%

Nanopore-based DNA sequencing sensors and CMOS readout approaches

Habibi

Dawji

Ghafar-Zadeh

et al. 2021

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“…It has provided a versatile promising platform in the creation of novel ncRNA biosensors [94] through sub-picomolarspecific biomarker detection [95]. Regarding the key role of ncRNAs in the early detection of cancers and diseases, several DNA-based biosensing tools have been developed and reviewed [96][97][98][99]. The biosensor must have the ability to convert a specific biological recognition event into a measurable signal.…”

Section: Biosensors For Liver Cancer-related Ncrna Detectionmentioning

confidence: 99%

Non-Coding RNA-Based Biosensors for Early Detection of Liver Cancer

et al. 2021

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Primary liver cancer is an aggressive, lethal malignancy that ranks as the fourth leading cause of cancer-related death worldwide. Its 5-year mortality rate is estimated to be more than 95%. This significant low survival rate is due to poor diagnosis, which can be referred to as the lack of sufficient and early-stage detection methods. Many liver cancer-associated non-coding RNAs (ncRNAs) have been extensively examined to serve as promising biomarkers for precise diagnostics, prognostics, and the evaluation of the therapeutic progress. For the simple, rapid, and selective ncRNA detection, various nanomaterial-enhanced biosensors have been developed based on electrochemical, optical, and electromechanical detection methods. This review presents ncRNAs as the potential biomarkers for the early-stage diagnosis of liver cancer. Moreover, a comprehensive overview of recent developments in nanobiosensors for liver cancer-related ncRNA detection is provided.

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“…Both Zahra Izadi et al [76] and Wei Sun et al [85] used methylene blue (MB) as the electroactive indicator to detect DNA hybridization efficiency, because MB can intercalated into the dsDNA structure and reaction signal was improved [54]. TB can bind to the negatively charged phosphate group of DNA.…”

Section: Electrochemical Dna Biosensorsmentioning

confidence: 99%

“…The bioreceptor, which specifically interacts with the target analyte, and the transducer, which converts this interaction into an electronic signal. Three basic parts of a biosensor are recognition material, transducer or detector system, and signal processor [54]. According to bioreceptors, biosensors can be classified into antibody biosensors, DNA biosensors, enzyme biosensors, whole-cell biosensors, and phage biosensor; according to transducers, biosensors can be classified into electrochemical biosensors, piezoelectric biosensors, calorimetric biosensors, and optical biosensors (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens

Zhang

Yang

et al. 2019

Sensors

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The vital importance of rapid and accurate detection of food borne pathogens has driven the development of biosensor to prevent food borne illness outbreaks. Electrochemical DNA biosensors offer such merits as rapid response, high sensitivity, low cost, and ease of use. This review covers the following three aspects: food borne pathogens and conventional detection methods, the design and fabrication of electrochemical DNA biosensors and several techniques for improving sensitivity of biosensors. We highlight the main bioreceptors and immobilizing methods on sensing interface, electrochemical techniques, electrochemical indicators, nanotechnology, and nucleic acid-based amplification. Finally, in view of the existing shortcomings of electrochemical DNA biosensors in the field of food borne pathogen detection, we also predict and prospect future research focuses from the following five aspects: specific bioreceptors (improving specificity), nanomaterials (enhancing sensitivity), microfluidic chip technology (realizing automate operation), paper-based biosensors (reducing detection cost), and smartphones or other mobile devices (simplifying signal reading devices).

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Electrochemical DNA biosensors: a review

Cited by 41 publications

References 184 publications

Nanopore-based DNA sequencing sensors and CMOS readout approaches

Nanopore-based DNA sequencing sensors and CMOS readout approaches

Non-Coding RNA-Based Biosensors for Early Detection of Liver Cancer

Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens

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