Electrochemical DNA sandwich biosensor based on enzyme amplified microRNA-21 detection and gold nanoparticles

Mandli, Jihane; Mohammadi, Hasna; Amine, Aziz

doi:10.1016/j.bioelechem.2017.03.002

Cited by 84 publications

(35 citation statements)

References 38 publications

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“…simply. [76][77][78][79][80][81] Second, compared with optical system settings, EIS system settings are small in size and easy to carry, and thus, this detection method can be tested not only in the laboratory but also outside the laboratory. Third, EIS is a powerful nondestructive technique and can be used in biological and medical tests.…”

Section: Eis Detection Methodsmentioning

confidence: 99%

“…Therefore, impedance detection of miRNA has been favored by researchers not only as strategy for developing electrochemical nucleic acid biosensors but also as a useful characterization and alternative method. [76][77][78][79][80][81][82][83] For the first time, Congur et al used chemically activated GO to modify pencil graphite electrodes (PGEs) to detect the impedance of miRNA-34a, a biomarker of Alzheimer's disease and various types of cancer. The PGE surface was chemically activated, and the complementary miRNA-34a DNA probe combined with the miRNA target was fixed on the GO-PGE surface.…”

Section: Eis Detection Methodsmentioning

confidence: 99%

“…Compared with other electrochemical methods, impedance detection biosensors have many advantages. First, it is a label‐free technique that can analyze molecules quickly, reliably, and simply . Second, compared with optical system settings, EIS system settings are small in size and easy to carry, and thus, this detection method can be tested not only in the laboratory but also outside the laboratory.…”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

“…Third, EIS is a powerful nondestructive technique and can be used in biological and medical tests. Therefore, impedance detection of miRNA has been favored by researchers not only as strategy for developing electrochemical nucleic acid biosensors but also as a useful characterization and alternative method …”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

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Progress in miRNA Detection Using Graphene Material–Based Biosensors

Zhang

Miao

Sun

et al. 2019

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MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs that play critical roles in physiologic and pathologic processes and are vital biomarkers for several disease diagnostics and therapeutics. Therefore, rapid, low‐cost, sensitive, and selective detection of miRNAs is of paramount importance and has aroused increasing attention in the field of medical research. Among the various reported miRNA sensors, devices based on graphene and its derivatives, which form functional supramolecular nanoassemblies of π‐conjugated molecules, have been revealed to have great potential due to their extraordinary electrical, chemical, optical, mechanical, and structural properties. This Review critically and comprehensively summarizes the recent progress in miRNA detection based on graphene and its derivative materials, with an emphasis on i) the underlying working principles of these types of sensors, and the unique roles and advantages of graphene materials; ii) state‐of‐the‐art protocols recently developed for high‐performance miRNA sensing, including representative examples; and iii) perspectives and current challenges for graphene sensors. This Review intends to provide readers with a deep understanding of the design and future of miRNA detection devices.

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Section: Eis Detection Methodsmentioning

confidence: 99%

Section: Eis Detection Methodsmentioning

confidence: 99%

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Zhang

Miao

Sun

et al. 2019

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“…It operates selectively owing to base-pairing features, If the sequence of bases containing certain known part of the nucleic acid molecule, then the complementary sequence of it can be synthesized (called as a probe) and labeled using an optically detectable agent (eg., a fluorescent label). It has shown great potential in the detection of several types of cancers [22,23], viral [24] infections, and genetic [25] disorders. The nucleic acid biosensors are developed on the principle that the singlestrand of the nucleic acid molecule has the capability to identify and bind to its complementary strand.…”

Section: Nucleic Acid Interactionsmentioning

confidence: 99%

Recent Advances and Applications of Biosensors in Novel Technology

Rajpoot¹

2017

Biosens J

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In recent time, several novel biosensors such as enzyme based, immunosensors, tissue-based, DNA biosensors, piezoelectric, and thermal biosensors have been explored with high sensitivity by many research groups. These biosensors exhibit many essential benefits, including operational simplicity, remarkable sensitivity, low-cost instrumentation, the potential of automation, inherent miniaturization. They have offered elegant paths for the detection of even trace amounts of analytical agents of biological significance existing from macromolecules (e.g., disease biomarkers and antigens) and small molecules (e.g., natural toxins and haptens) to cells, viruses, and bacteria. Many electrochemical techniques viz., amperometry and voltammetry, photoelectrochemistry, electrochemiluminescence, impedance, piezoelectricity, potentiometry, alternating current electrohydrodynamics, and field-effect transistor have been utilized in the development of immunoassays to attain high sensitivity for electrochemical signal transduction. Recent developments in biological methods and instrumentation after using fluorescence tag to various nanocarriers such as nanoparticles, nanowires, nanotubes, etc. have improved the sensitivity of biosensors. Utilization of nucleotides/aptamers, affibodies, molecule imprinted polymers, and peptide arrays offer great tools to prepare advanced biosensors. Merging of nanotechnology with biosensor systems enhanced the diagnostic capability. This review gives an outline of the advances in biosensors technology relating biomedical sciences.

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Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

Erdem

Çongur

2017

Electroanalysis

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Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequenceselective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP-PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non-complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1 : 1) or target: MM (1 : 1).

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Electrochemical DNA sandwich biosensor based on enzyme amplified microRNA-21 detection and gold nanoparticles

Cited by 84 publications

References 38 publications

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Recent Advances and Applications of Biosensors in Novel Technology

Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

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