Electrochemical Enzyme Sensor Based on the Two-Dimensional Metal–Organic Layers Supported Horseradish Peroxidase

Abstract

Metal–organic frames (MOFs) have recently been used to support redox enzymes for highly sensitive and selective chemical sensors for small biomolecules such as oxygen (O2), hydrogen peroxide (H2O2), etc. However, most MOFs are insulative and their three-dimensional (3D) porous structures hinder the electron transfer pathway between the current collector and the redox enzyme molecules. In order to facilitate electron transfer, here we adopt two-dimensional (2D) metal–organic layers (MOLs) to support the HRP molecules in the detection of H2O2. The correlation between the current response and the H2O2 concentration presents a linear range from 7.5 μM to 1500 μM with a detection limit of 0.87 μM (S/N = 3). The sensitivity, reproducibility, and stability of the enzyme sensor are promoted due to the facilitated electron transfer.

Metal-Organic Frameworks: Versatile Platforms for Biomedical Innovations

“…MOFs have proven very challenging in detecting and separating molecules in the diagnostic realm, especially in diagnosing conditions such as neurological disorders and epilepsy. The work of Zhang et al [ 47 ] illustrates this versatility, where they co-encapsulated multienzyme systems in MOFs for the specific detection of lactate.…”

“…Significant improvements in current response have been observed when using 2D metal-organic layers (MOLs) to support redox enzymes, as shown by Xiong et al [ 47 ]. MOFs functionalized with specific antigens have also been used to detect antibodies, such as anti-HIV antibodies.…”

“…MOFs have proven very challenging in detecting and separating molecules in the diagnostic realm, especially in diagnosing conditions such as neurological disorders and epilepsy. The work of Zhang et al [47] illustrates this versatility, where they co-encapsulated multienzyme systems in MOFs for the specific detection of lactate. MOFs have proven very challenging in detecting and separating molecules in the diagnostic realm, especially in diagnosing conditions such as neurological disorders and epilepsy.…”