Electrochemical genomagnetic assay for the detection of hepatitis B virus DNA in polymerase chain reaction amplicons by using disposable sensor technology

Influence of pencil lead hardness on voltammetric response of graphite reinforcement carbon electrodes

“…The literature describes many applications of GRC electrodes: for determining the titratable acidity in vinegar [3,4]; in abrasive stripping voltammetry [5]; in determination of ozone [6], dopamine [7], caffeine [8], trepibutone [9], uric acid [10]; in simultaneous determination of nitrite, dopamine and serotonin [11]; as substrate for mercury [12] and bismuth [13] films in trace metal determination; in liquid chromatography for determination of phenols [14]; applied with modified surface for determination of 2,4-dichlorophenol [15]; in voltammetry of microparticles [16]; in studies of copper-chelating properties of flavonoids [17]; in studies of DNA hybridization [18][19][20][21]; for discrimination of herpes type [22]; in detection of hepatitis B virus DNA [23,24] and in studies of interaction between DNA and lycorine [25], arsenic trioxide [26] and cis-DDP [27] molecules.…”

Electrochemical-Nucleic Acid Detection with Enhanced Specificity and Sensitivity

“…These methods do provide sufficient results, they prove to be time-consuming and require SOTA laboratories, which most developing countries do not have. In addition, just PCR technology can experience false positive readings between 9 and 57% [11] and ultimately not meet the criteria of the WHO of being specific and sensitive. Electrochemical detection offers greater sensitivity of target at low concentration [18][19][20][21][22][23].…”

“…Nucleic Acid electrochemical sensors convert the hybridization event of congruent base pairing that occurs on the electrode surface to a useful electrical signal [10,11]. The electrochemical biosensor is a technology that addresses some of the criteria imposed by the WHO and the ASSURED requirements.…”

Electrochemical DNA sensor for simultaneous detection of genes encoding two functional enzymes involved in lignin degradation

“…The combination of PCR amplification and DNA sensor was also utilized to improve the detection sensitivity [26,27]. Our group has developed an electrochemical DNA sensor based on the sandwich hybridization recognition of target sequence of lignin peroxidase genes [28], and the simultaneous determination of lignin peroxidase and manganese peroxidase activities using artificial neural networks was also studied [29].…”