Electrochemical imaging for cell analysis in microphysiological systems

Konno, An; Ino, Kosuke; Utagawa, Yoshinobu; Shiku, Hitoshi

doi:10.1016/j.coelec.2023.101270

Cited by 6 publications

(1 citation statement)

References 64 publications

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“…When image-related information is extracted, SPMs can reveal important physical structures or map specific biomolecules, providing deeper insight into prokaryotes and eukaryotes. Various SPM setups are available for investigating electrochemical systems, including many types such as scanning electrochemical microscopy (SECM), scanning ion-conductance microscopy (SICM), scanning electrochemical cell microscopy (SECCM), and atomic force microscopy (AFM) [6][7][8][9][10][11][12][13][14]. The scanning mechanism of SPMs is based on a probe that is scanned across a sample surface, and the probe-sample interaction is monitored by sensing changes in the local environment.…”

Section: Introductionmentioning

confidence: 99%

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

Lin

Darvishi

2023

Biosensors

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Bacteria are similar to social organisms that engage in critical interactions with one another, forming spatially structured communities. Despite extensive research on the composition, structure, and communication of bacteria, the mechanisms behind their interactions and biofilm formation are not yet fully understood. To address this issue, scanning probe techniques such as atomic force microscopy (AFM), scanning electrochemical microscopy (SECM), scanning electrochemical cell microscopy (SECCM), and scanning ion-conductance microscopy (SICM) have been utilized to analyze bacteria. This review article focuses on summarizing the use of electrochemical scanning probes for investigating bacteria, including analysis of electroactive metabolites, enzymes, oxygen consumption, ion concentrations, pH values, biofilms, and quorum sensing molecules to provide a better understanding of bacterial interactions and communication. SECM has been combined with other techniques, such as AFM, inverted optical microscopy, SICM, and fluorescence microscopy. This allows a comprehensive study of the surfaces of bacteria while also providing more information on their metabolic activity. In general, the use of scanning probes for the detection of bacteria has shown great promise and has the potential to provide a powerful tool for the study of bacterial physiology and the detection of bacterial infections.

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Section: Introductionmentioning

confidence: 99%

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

Lin

Darvishi

2023

Biosensors

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Porous membranes integrated into electrochemical systems for bioanalysis

Ino,

Utagawa,

Hiramoto

et al. 2024

Electrochemical Science Adv

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Porous membranes have emerged as promising platforms for bioanalysis because of their unique properties including high surface area, selective permeability, and compatibility with electrochemical techniques. This minireview presents an overview of the development and applications of porous membrane‐based electrochemical systems for bioanalysis. First, we discuss the existing fabrication methods for porous membranes. Next, we summarize electrochemical detection strategies for bioanalysis using porous membranes. Electrochemical biosensors and cell chips fabricated from porous membranes are discussed as well. Furthermore, porous micro‐/nanoneedle devices for bioapplications are described. Finally, the utilization of scanning electrochemical microscopy for cell analysis on porous membranes and electrochemiluminescence sensors is demonstrated. Future perspectives of the described membrane detection strategies and devices are outlined in each section. This work can help enhance the performance of porous membrane‐based electrochemical systems and expand the range of their potential applications.

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Editorial Overview: Bioelectrochemistry (2023)Bioelectrochemistry: Post-Pandemic Biosensor Research for Biomedical Applications

Ferapontova,

Kraatz

2023

Current Opinion in Electrochemistry

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Electrochemical imaging for cell analysis in microphysiological systems

Cited by 6 publications

References 64 publications

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

A Brief Review of In Situ and Operando Electrochemical Analysis of Bacteria by Scanning Probes

Porous membranes integrated into electrochemical systems for bioanalysis

Editorial Overview: Bioelectrochemistry (2023)Bioelectrochemistry: Post-Pandemic Biosensor Research for Biomedical Applications

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