Electrochemical imaging of cells and tissues

Lin, Tzu‐En; Rapino, Stefania; Girault, Hubert H.; Lesch, Andreas

doi:10.1039/c8sc01035h

Cited by 76 publications

(59 citation statements)

References 73 publications

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“…16 The popularity of SECM, in particular, and SEPM in general, for single cell electrochemical imaging and life sciences applications, is reflected in a number of recent review articles. [17][18][19][20][21][22] SEPM is a key enabling technology for the emerging fields of single entity electrochemistry and electrochemistry at nano-interfaces, for which there were seminal Faraday Discussion meetings in 2016 and 2018, respectively. The published volumes of these meetings 23, 24 contain not only original papers, but also extensive discussions of key issues.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Electrochemical Mapping

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Nanoscale Electrochemical Mapping

et al. 2018

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“…In the near future, this imaging technique will be used to monitor the release of dopamine from cells in the presence of ascorbic acid without potential scanning, e.g., by fast-scan voltammetry, although the interaction between dopamine and ascorbic acid during electrochemical detection would have to be carefully investigated. Previously, electrode arrays were applied to three-dimensional tissue organs; hence, (23)(24)(25)(26) we also aim to investigate the current system for possible application in this regard, such as in organs-on-a-chip. Figure 6(a) shows amperograms for various concentrations of PAP and PAPP.…”

Section: Resultsmentioning

confidence: 99%

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Ino¹,

Onodera²,

Nashimoto³

et al. 2019

Sensors and Materials

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“…In the present study an electrochemical method for quantification of ROS production upon activation of the respiratory chain in a suspension of human cells is presented; increased generation of ROS by mitochondrial respiratory complexes and their release to cytosol was also documented in two cellular models of human mitochondrial diseases. The advantages of the described method are: (i) relative low amounts of cells required for the analysis, which is an important feature when samples from patients are investigated); (ii) the feasibility of the described method for the access to the internal districts of cells that can be universally used on a huge type of cells; iii) the robustness of the electrochemical measurements in living system,,,, also characterised by an high spatial and temporal resolution;,,, iv) in the presented method the species of interest are directly detected at the microelectrode and the production and the scavenging phenomena of the reactive species can be followed in real time; v) it does not require for the addition of exogenous molecular probes that can be affected by chain reactions, not specific reactions and that can interfere with the physiology/pathology of the cells under investigation.…”

Section: Figurementioning

confidence: 99%

Reactive Oxygen Species Produced by Mutated Mitochondrial Respiratory Chains of Entire Cells Monitored Using Modified Microelectrodes

et al. 2019

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Genetic alterations affecting subunits of the mitochondrial respiratory chain complexes often impair their catalytic activities and result in enhanced production of reactive oxygen species (ROS). An electrochemical setup was employed to quantify mitochondrial ROS production in plasma membranepermeabilized cellular models of two genetic diseases: the Δcytb cell line bearing a microdeletion in the mitochondrial MT-CYB gene causing a severe encephalomyopathy and the RJ206 cell line, harbouring a pathogenic mutation associated with Leber's hereditary optic neuropathy. The responses of black platinum modified microelectrodes to the most common cellular redox buffers, namely, NADH and glutathione, as well as substrates deriving from the oxidative metabolism of glucose, were investigated; a relatively high sensitivity, although lower than that for ROS, was shown for NADH. Time-resolved amperometric measurements of ROS production upon respiratory chain activation at high NADH/NAD + ratio revealed a 50 % and 100 % increase of ROS in cells bearing defective complex I and complex III, respectively, as compared to wild type cells.Reactive oxygen species (ROS) have been shown to play a main role both in physiological and pathological processes in human cells. [1,2] Other than actively participating in maintenance of redox homeostasis and being involved in oxidative stress, with pathological consequences as tumor initiation and expansion or ageing, [3,4] they can also act as signalling molecules in several physiological processes, e. g. cell self-renewal and immunity. [1,5,6] Various methodologies have been developed to track or quantify ROS production in cells; [7,8] they can be classified mainly into four groups, with relation to the methodological approach applied, i. e. electron spin resonance (ESR) probes, fluorescent probes, genetic sensors or electrochemical methods.Each of these techniques suffers from some intrinsic limitations: low specificity and limited quantitative information have been questioned for the commonly used fluorescent probes. [8,9] Expensive, high-level research equipment and not trivial sample preparation are the main limitation of ESR methods, while the relevant time required for generation of a genetic tool is a strong drawback for genetic sensors. Electrochemical methods combine the capability of discriminating different redox active species as a function of the applied potential at the working electrode with the possibility of following quantitatively their time course. [7] However limitations, as compared to other techniques come from difficulties to investigate internal cell compartments, even if they have been recently challenged by development of nanoelectrodes able to probe cell cytoplasm [10] or intracytoplasmatic vesicles [11] of living cells.Microelectrode modified by the electrodeposition of nanoporous black platinum (BP) have been shown to be excellent electrochemical tools for the detection in living cells of ROS, but also reactive nitrogen species (RNS). [7,[12][13][14] Their ability ...

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Electrochemical imaging of cells and tissues

Abstract: This minireview summarizes the recent achievements of electrochemical imaging platforms to map cellular functions in biological specimens using electrochemical scanning nano/micro-probe microscopy and 2D chips containing microelectrode arrays.

Cited by 76 publications

References 73 publications

Nanoscale Electrochemical Mapping

Nanoscale Electrochemical Mapping

Differential Electrochemicolor Imaging Using LSI-based Device for Simultaneous Detection of Multiple Analytes

Reactive Oxygen Species Produced by Mutated Mitochondrial Respiratory Chains of Entire Cells Monitored Using Modified Microelectrodes

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