Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors

Abe, Hiroya; Ino, Kosuke; Li, Chen Zhong; Kanno, Yusuke; Inoue, Kumi Y.; Suda, Akira; Kunikata, Ryota; Matsudaira, Masahki; Takahashi, Yasufumi; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1021/acs.analchem.5b01307

Cited by 68 publications

(54 citation statements)

References 39 publications

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“…As an imaging tool, the size of the device, and inherently needed electrode separation distance, will limit its use. Due to the limitations of device size and structure, this type of imaging will typically have lower resolution [134]. Matsue and coworkers [135] used an amperometric sensor array device at a size-scale suited to cell clusters.…”

Section: Microelectrode Arrays and Large-scale Integration Chipsmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

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This review discusses a broad range of recent advances (2013)(2014)(2015)(2016)(2017) in chemical imaging using electrochemical methods, with a particular focus on techniques that have been applied to study cellular processes, or techniques that show promise for use in this field in the future. Non-scanning techniques such as microelectrode arrays (MEAs) offer high time-resolution (<10 ms) imaging; however, at reduced spatial resolution. In contrast, scanning electrochemical probe microscopies (SEPMs) offer higher spatial resolution (as low as a few nm per pixel) imaging, with images collected typically over many minutes. Recent significant research efforts to improve the spatial resolution of SEPMs using nanoscale probes and to improve the temporal resolution using fast scanning have resulted in movie (multiple frame) imaging with frame rates as low as a few seconds per image. Many SEPM techniques lack chemical specificity or have poor selectivity (defined by the choice of applied potential for redox-active species). This can be improved using multifunctional probes, ion-selective electrodes and tip-integrated biosensors, although additional effort may be required to preserve sensor performance after miniaturization of these probes. We discuss advances to the field of electrochemical imaging, and technological developments which are anticipated to extend the range of processes that can be studied. This includes imaging cellular processes with increased sensor selectivity and at much improved spatiotemporal resolution than has been previously customary.

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Section: Microelectrode Arrays and Large-scale Integration Chipsmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

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“…CV is performed by cycling the potential between the working electrode and the reference electrode, and measuring the resulting current. This technique is widely used in the development of cost-effective, simple and rapid methods for Ab detection [12]. There are several research studies that used CV as their main tool and have obtained great results.…”

Section: Cyclic Voltammetry (Cv)mentioning

confidence: 99%

β-Amyloid Biomarker Detection for Alzheimer’s Disease

Grajales

Shuang

et al. 2017

J. Anal. Test.

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Alzheimer's disease (AD) is a neurodegenerative disease. It is a type of dementia and it mainly deals with thinking, memory and behavioral aspects. It is a progressive disease, which in course of time damages most sections of the brain. Beta amyloid peptides (Ab) have been recognized as potential biomarkers to monitor AD. The Ab protein is made up of 39-42 amino acids, which are the main components of the plaques found in the AD brain. This beta amyloid is toxic to the neurons and causes degeneration. In order to measure these biomarkers, nanobiosensing techniques are used. Timely monitoring of Ab levels helps in the detection of the irreversible disease called AD. These techniques fall in two categories, viz., in vitro and in vivo. Several techniques like electrochemical techniques, cantilever-based liposome biosensor, ELISA, PET scan, microdialysis, etc., are discussed further in the article. These techniques can prove to be effective methods for quantifying amyloid deposition within specific brain regions.

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“…By coating the sensor surface with gel polymer containing horseradish peroxidase, the production and diffusion of H 2 O 2 catalyzed by glucose oxidase was clearly visualized. (9) Reported applications of the BioLSI includes the characterization of an embryoid body by monitoring the activity of alkaline phosphatase, (10) the visualization of dopamine release from spheroid cells, (11) and conductivity imaging. (12) …”

Section: Biolsimentioning

confidence: 99%

Development and Bio-imaging Applications of a Chemical Imaging Sensor

2016

Sensors and Materials

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A chemical imaging sensor is a field-effect-based semiconductor sensor, capable of label-free imaging of ion distributions in a solution. The sensor has attracted much interest owing to its simple structure and diverse fields of application of bioimaging. In this article, the principle and development of the chemical imaging sensor are summarized, compared with those of other labelfree imaging sensors. Bio-imaging applications of the chemical imaging sensor to ion diffusion, enzymatic reaction, and metabolic activities of microorganisms are also reviewed.

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Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors

Cited by 68 publications

References 39 publications

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

β-Amyloid Biomarker Detection for Alzheimer’s Disease

Development and Bio-imaging Applications of a Chemical Imaging Sensor

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