Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: An anticipated analytical tool for food safety

Hervás, Miriam; López, Miguel Ángel; Escarpa, Alberto

doi:10.1016/j.aca.2009.09.024

Cited by 63 publications

(20 citation statements)

References 52 publications

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“…Magnetic bead usage within the food toxicology field mainly concern magnetic bead-based immunoassays. Examples of such assays include a fluorescence-based immunoassay for staphylococcal enterotoxin B, such as the one reported by [22]; an immunomagnetic bead assay for botulinum neurotoxin types C and D [23]; an electrochemical based immunoassay for zearalenone reported by Hervás et al [24]; an enzyme linked immuno-magnetic electrochemical (ELIME) methods for mycotoxin detection [25] and aflatoxin B-1 in corn samples [26]. Therefore, the use of magnetic microsphere immunoassays for the detection of PSP toxins may be a potential area for future research.…”

Section: Resultsmentioning

confidence: 99%

Studies in the Use of Magnetic Microspheres for Immunoaffinity Extraction of Paralytic Shellfish Poisoning Toxins from Shellfish

Devlin

Campbell

Kawatsu

et al. 2011

Toxins

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Paralytic shellfish poisoning (PSP) is a potentially fatal human health condition caused by the consumption of shellfish containing high levels of PSP toxins. Toxin extraction from shellfish and from algal cultures for use as standards and analysis by alternative analytical monitoring methods to the mouse bioassay is extensive and laborious. This study investigated whether a selected MAb antibody could be coupled to a novel form of magnetic microsphere (hollow glass magnetic microspheres, brand name Ferrospheres-N) and whether these coated microspheres could be utilized in the extraction of low concentrations of the PSP toxin, STX, from potential extraction buffers and spiked mussel extracts. The feasibility of utilizing a mass of 25 mg of Ferrospheres-N, as a simple extraction procedure for STX from spiked sodium acetate buffer, spiked PBS buffer and spiked mussel extracts was determined. The effects of a range of toxin concentrations (20-300 ng/mL), incubation times and temperature on the capability of the immuno-capture of the STX from the spiked mussel extracts were investigated. Finally, the coated microspheres were tested to determine their efficiency at extracting PSP toxins from naturally contaminated mussel samples. Toxin recovery after each experiment was determined by HPLC analysis. This study on using a highly novel immunoaffinity based extraction procedure, using STX as a model, has indicated that it could be a convenient alternative to conventional extraction procedures used in toxin purification prior to sample analysis.

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Section: Resultsmentioning

confidence: 99%

Studies in the Use of Magnetic Microspheres for Immunoaffinity Extraction of Paralytic Shellfish Poisoning Toxins from Shellfish

Devlin

Campbell

Kawatsu

et al. 2011

Toxins

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“…Also, the possibility of using electrochemical detection coupled with a MBs-based immunoassay for the detection of ZEA in baby food was demonstrated [36,37]. Subsequently, the developed system was integrated in microfluidic chips following the scheme displayed in Figure 4, where the simple channel layout of a double-T microchip was used to perform sequentially the immunoreaction and the enzyme reaction by applying a program of electric fields [38].…”

Section: Detection Of Biological Toxinsmentioning

confidence: 99%

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

2011

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Nowadays, detecting the presence of bioterrorism and biohazard agents in environmental and food samples is of great concern, due to their toxicity, and because many of them are prone to be used in terrorism attacks. The use of functionalized magnetic beads (MBs) in the development of electrochemical immuno-and genosensors has resulted in innovative and powerful detection strategies that may be applied to environmental, food and clinical analysis. This review describes current research on the combination of functionalized MBs with electrochemical detection for the development of magnetobiosensors applied to rapid, sensitive and specific detection of bioterrorism and biohazard agents.

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“…The ZEA has been associated with precocious puberty, hyperplasic and neoplastic endometrium, and human cervical cancer in humans. [8] Thus, certain authorities, for example the FAO=WHO Joint Expert Committee on Food Additives of the United Nations (JECFA) and the European Food Safety Authority (EFSA), emphasized the importance of the regulations for ZEA in food and feed samples. Several other nations have established maximum tolerances ranging from 50 mg=kg to 1000 mg=kg in foods while the US Food and Drug Administration has not established a guideline for an acceptable level of ZEA.…”

Section: Introductionmentioning

confidence: 99%

“…The current maximum levels set by the European Commission are 20, 75, and 100 mg=kg for baby food, cereal flour, and unprocessed cereals, respectively. [8,[12][13][14] Additionally, maximum levels of ZEA by the Official Journal of the European Union is 2 mg kg À1 for feed materials from the cereals and cereal products with the exception of maize by-products, and 3 mg=kg for feed materials from maize products. [15] There is a need to develop sensitive and specific methods for rapid and economic ZEA detection in food and feed samples due to the important implications about the effect of ZEA on both human and animal health.…”

Section: Introductionmentioning

confidence: 99%

Determination of Zearalenone by the Capillary Zone Electrophoresis-Uv Detection and Its Application to Poultry Feed and Cereals

Güray

Tunçel

Uysal

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20 mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15 kV, injecting 10 s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254 nm. Zearalanon and internal standard appeared at 5.46 min (RSD% 0.20) and 6.35 min (RSD% 0.18), respectively. The repeatability results are in the range of 0.01-1.58 for inter-day. The linearity was investigated in the range of 5.20 Â 10 À7 M to 7.86 Â 10 À5 M ZEA and it was linear fitting to the equation of [rPN ¼ 244803.8 C(M) À0.0348; r ¼ 0.9999]. The LOD and LOQ were calculated to be 2.70 Â 10 À8 M (8.25 lg=L) and 8.17 Â 10 À8 M (25 lg=L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.

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Electrochemical immunoassay using magnetic beads for the determination of zearalenone in baby food: An anticipated analytical tool for food safety

Cited by 63 publications

References 52 publications

Studies in the Use of Magnetic Microspheres for Immunoaffinity Extraction of Paralytic Shellfish Poisoning Toxins from Shellfish

Studies in the Use of Magnetic Microspheres for Immunoaffinity Extraction of Paralytic Shellfish Poisoning Toxins from Shellfish

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

Determination of Zearalenone by the Capillary Zone Electrophoresis-Uv Detection and Its Application to Poultry Feed and Cereals

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