2014
DOI: 10.1016/j.bios.2013.12.030
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Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum

Abstract: This paper describes the development of an immunosensor for detection of anti-hemagglutinin antibodies. Its preparation consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibody, (iv) immobilization of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA. The interactions between two variants of recombinant HA … Show more

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Cited by 75 publications
(48 citation statements)
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“…The immunosensor based on glassy carbon electrode incorporating the protein " has been successfully applied to distinguish between sera of unvaccinated and vaccinated chickens against the avian inluenza virus. Sensitivity of EIS immunosensor was almost 10 4 times much beter than ELIS" [8].…”
Section: Electrochemical Immunosensors Methods For Immobilizatimentioning
confidence: 93%
See 1 more Smart Citation
“…The immunosensor based on glassy carbon electrode incorporating the protein " has been successfully applied to distinguish between sera of unvaccinated and vaccinated chickens against the avian inluenza virus. Sensitivity of EIS immunosensor was almost 10 4 times much beter than ELIS" [8].…”
Section: Electrochemical Immunosensors Methods For Immobilizatimentioning
confidence: 93%
“…Taking into account the immunosensor sensitivity with detection limit of 0. pg/mL and speciicity negative control H7 hemagglutinin generate negligible response , they could be recommended for direct electrochemical detection of H5 hemagglutinin from inluenza virus in the ield conditions. Direct impedimetric immunosensor has been successfully applied for detection of antibodies generated against H5N1 virus [8]. The base of this immunosensor was glassy carbon electrode incorporated fragment of H5 hemagglutinin.…”
Section: Methods Of Detection Of Immunoreaction: Selected Examplementioning
confidence: 99%
“…In our previous paper [22], we have described an impedimetric immunosensor for the detection of anti-H5 HA antibodies. Its preparation consisted of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with the EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibodies, (iv) immobilization of the His 6 -H5 HA, and (v) filling any free space with BSA.…”
Section: Detection Of the Anti-hemagglutinin Antibodies In Hen Seramentioning
confidence: 99%
“…Electrochemical impedance spectroscopy (EIS) has also proved to be an effective method for monitoring the interfacial properties of the biochemical process and binding events occurring on the electrode's surface e.g., antigen-antibodies conjugation [39,40]. An impedimetric immunosensor for the fast determination of OTA in red wine samples was developed by using instant catalyst-promoted precipitation amplification process [41].…”
Section: Biosensors For the Detection Of Ochratoxin A In Wine Beer Amentioning
confidence: 99%