Electrochemical immunosensor for determination of cardiac troponin I using two-dimensional metal-organic framework/Fe3O4–COOH nanosheet composites loaded with thionine and pCTAB/DES modified electrode

Ahmadi, Anita; Khoshfetrat, Seyyed Mehdi; Mirzaeizadeh, Zahra; Kabiri, Shima; Rezaie, Javad; Omidfar, Kobra

doi:10.1016/j.talanta.2021.122911

Cited by 40 publications

(10 citation statements)

References 37 publications

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“…In the gradient concentration (0.02–20 ng mL –1 ) spiked recovery test, no outliers and false positive/negative results were found to occur, and the test results showed a high degree of consistency between the two methods, suggesting that the developed test protocol was able to accurately determine the concentration of cTnI in the actual samples from humans (Figure E). , In addition, clinical samples collected from potential patients were evaluated for accuracy according to the test protocol and the ELISA kit, and again no significant differences were found between the two test protocols, and the t exp were both less than 2.78, thus further defining the accuracy of the developed test protocol for evaluating cTnI targets (Table S4). In addition, a comparison of the test protocols that had reported for cTnI targets revealed that the game theory-based test protocol we developed could match or even outperform them in terms of dynamic response range and detection limit (Figure F). – …”

Section: Resultsmentioning

confidence: 99%

Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Chen,

Yu,

Wang

et al. 2023

Anal. Chem.

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Noble-metal nanozymes have demonstrated great potential in various fields. However, aggregation of single-particle nanoparticles severely affects their exposed catalytically active sites to the extent of exhibiting weak enzyme-like activity. Here, we present an organic block surfactant (polyvinylpyrrolidone, PVP) to construct monodisperse water-stable Pt nanoclusters (Pt NCs) for an enhanced immunoassay of cardiac troponin I (cTnI). The PVP-modified Pt NC nanozyme exhibited up to 16.3 U mg–1 peroxidase-mimicking activity, which was mainly attributed to the ligand modification on the surface and the electron-absorbing effect of the ligand on the Pt NCs. The PVP-modified Pt NCs have a lower OH-transition potential, as determined by density functional theory. Under optimized experimental conditions, the enhanced nanozyme immunoassay strategy exhibited an ultrawide dynamic response range of 0.005–50 ng mL–1 for cTnI targets with a detection limit of 1.3 pg mL–1, far superior to some reported test protocols. This work provides a designable pathway for the design of artificial enzymes with high enzyme-like activity to further expand the practical range of enzyme alternatives.

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Section: Resultsmentioning

confidence: 99%

Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Chen,

Yu,

Wang

et al. 2023

Anal. Chem.

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“…On the other hand, Troponin I, an inhibitory protein within the troponin-tropomyosin complex, consists of 209 amino acids with a weight of 23 kDa. Its isoelectric point is 9.87. − …”

Section: Cardiac Biomarkersmentioning

confidence: 99%

Biosensing Platforms for Cardiac Biomarker Detection

Gerdan,

Saylan,

Denizli

2024

ACS Omega

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Myocardial infarction (MI) is a cardiovascular disease that occurs when there is an elevated demand for myocardial oxygen as a result of the rupture or erosion of atherosclerotic plaques. Globally, the mortality rates associated with MI are steadily on the rise. Traditional diagnostic biomarkers employed in clinical settings for MI diagnosis have various drawbacks, prompting researchers to investigate fast, precise, and highly sensitive biosensor platforms and technologies. Biosensors are analytical devices that combine biological elements with physicochemical transducers to detect and quantify specific compounds or analytes. These devices play a crucial role in various fields including healthcare, environmental monitoring, food safety, and biotechnology. Biosensors developed for the detection of cardiac biomarkers are typically electrochemical, mass, and optical biosensors. Nanomaterials have emerged as revolutionary components in the field of biosensing, offering unique properties that significantly enhance the sensitivity and specificity of the detection systems. This review provides a comprehensive overview of the advancements and applications of nanomaterial-based biosensing systems. Beginning with an exploration of the fundamental principles governing nanomaterials, we delve into their diverse properties, including but not limited to electrical, optical, magnetic, and thermal characteristics. The integration of these nanomaterials as transducers in biosensors has paved the way for unprecedented developments in analytical techniques. Moreover, the principles and types of biosensors and their applications in cardiovascular disease diagnosis are explained in detail. The current biosensors for cardiac biomarker detection are also discussed, with an elaboration of the pros and cons of existing platforms and concluding with future perspectives.

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“…The importance of food and water safety in various industries has led to the mining and improvement of nanoscale analytical devices known as nanobiosensors. Due to the numerous advantages of these devices such as portability, low cost, rapid assay time, ease of use, and high selectivity and sensitivity (Terry et al, 2005;Ahmadi et al, 2022;Khoshfetrat et al, 2022;Saeidi et al, 2022), particularly for the detection of infectious agents and pollutants in the environment (Pejcic et al, 2006;Sin et al, 2014;Ahmadi et al, 2021c), focus on the fabrication of biosensors as a diagnostic technology for the detection of different analytes in food, water, and environmental samples has intensely increased (Terry et al, 2005;Pejcic et al, 2006;Metkar and Girigoswami, 2018;Salouti and Khadivi derakhshan, 2020) (Figure 1).…”

Section: Biosensors: Development and Typesmentioning

confidence: 99%

Toward waterborne protozoa detection using sensing technologies

et al. 2023

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Drought and limited sufficient water resources will be the main challenges for humankind during the coming years. The lack of water resources for washing, bathing, and drinking increases the use of contaminated water and the risk of waterborne diseases. A considerable number of waterborne outbreaks are due to protozoan parasites that may remain active/alive in harsh environmental conditions. Therefore, a regular monitoring program of water resources using sensitive techniques is needed to decrease the risk of waterborne outbreaks. Wellorganized point-of-care (POC) systems with enough sensitivity and specificity is the holy grail of research for monitoring platforms. In this review, we comprehensively gathered and discussed rapid, selective, and easy-to-use biosensor and nanobiosensor technologies, developed for the early detection of common waterborne protozoa.

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Electrochemical immunosensor for determination of cardiac troponin I using two-dimensional metal-organic framework/Fe3O4–COOH nanosheet composites loaded with thionine and pCTAB/DES modified electrode

Cited by 40 publications

References 37 publications

Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Block-Polymer-Restricted Sub-nanometer Pt Nanoclusters Nanozyme-Enhanced Immunoassay for Monitoring of Cardiac Troponin I

Biosensing Platforms for Cardiac Biomarker Detection

Toward waterborne protozoa detection using sensing technologies

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