Electrochemical immunosensor for highly sensitive detection of cTnI via in-situ initiated ROP signal amplification strategy

Cheng, Di; Zhou, Zhenbo; Shang, Shasha; Wang, Hesen; Guan, Huaimin; Yang, Huaixia; Liu, Yanju

doi:10.1016/j.aca.2022.340032

Cited by 17 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…-With the gradual modi cation of Ab 1 (curve e), BSA (curve f), and Ag (curve g), the semicircle diameter gradually increased, which might be associated with these substances being proteins. Fe (CN) 6 3−/4− in as hindered to the electrode surface by insulating protein molecules, which was in agreement with the results of Cheng et al [34], which found that the protein increases the electron transfer resistance. After Ab 2 was incubated on the electrode, the semicircular diameter increased further (curve h).…”

Section: Electrochemical Characterization Of the Immunosensorsupporting

confidence: 92%

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

Qian,

Lu,

Zheng

et al. 2023

Preprint

View full text Add to dashboard Cite

In this study, a sandwich immunosensor based on the conjugate of gold nanoparticles and quantum dot microspheres on polypyrrole/polyaniline (PPY-PANI) composite membrane was developed for the detection of Escherichia coli O157:H7 (E. coli O157:H7). Quantum dot microsphere conjugates (QBC), as immunoprobes throughout electrochemical detection, exhibited signal amplifications. Each stage of the immunosensor preparation was characterized by cyclic voltammetry, and electrochemical impedance spectroscopy. The results showed that the optimal reaction conditions of antigen and secondary antibody were 37 ℃ and 60 min respectively, and the concentration of secondary antibody was 0.01 mg·mL− 1. When E. coli O157:H7 was performed in the immunosensor of PPY-PANI-Ab2, E. coli O157:H7 in the concentration range of 4.73×102-4.73×109 CFU·mL− 1, exhibited a linear relationship between the change of current (ΔIpa) and the logarithm of bacterial concentration. Compared to 100 CFU·mL− 1 of the limit of detection (LOD) in normal saline solution, the lowest detection limit in 0.1% peptone water was 113 CFU·mL− 1, and the signal-to-noise ratio was 3. Moreover, the recovery rate of spiked milk and shrimp were 80.80-98.36% and 75.20-94.31%, and the relative standard deviation (RSD) was 3.03–6.18% and 1.61–6.94% respectively. Therefore, the immunosensor of with low cost and good stability is expected to be used for rapid screening of E. coli O157:H7 in food.

show abstract

Section: Electrochemical Characterization Of the Immunosensorsupporting

confidence: 92%

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

Qian,

Lu,

Zheng

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In recent years, signal enhancement strategies have been used for analytical signal amplification in sensing to improve the sensitivity of sensors, such as enzyme catalysis [12], polymer chains [13], and nanomaterials [14,15]. Among them, enzyme-catalyzed reactions with high efficiency, high catalytic activity, and good specificity have attracted a lot of attention from relevant researchers.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive fluorescence sensor for tobacco mosaic virus RNA based on DSN cycle and ARGET ATRP double signal amplification

Jin,

Ma,

Zhang

et al. 2024

Luminescence

Self Cite

View full text Add to dashboard Cite

Early and sensitive detection of tobacco mosaic virus (TMV) is of great significance for improving crop yield and protecting germplasm resources. Herein, we constructed a novel fluorescence sensor to detect TMV RNA (tRNA) through double strand specific nuclease (DSN) cycle and activator regenerative electron transfer atom transfer radical polymerization (ARGET ATRP) dual signal amplification strategy. The hairpin DNA complementarily paired with tRNA was used as a recognition unit to specifically capture tRNA. By the double‐stranded DNA hydrolyzed with DSN, tRNA is released to open more hairpin DNA, and more complementary DNA (cDNA) is bound to the surface of the magnetic beads (MBs) to achieve the first amplification. After binding with the initiator, the cDNA employed ARGET ATRP to attach more fluorescent signal molecules to the surface of MBs, thus achieving the second signal amplification. Under the optimal experimental conditions, the logarithm of fluorescence intensity versus tRNA concentration showed a good linear relationship in the range of 0.01–100 pM, with a detection limit of 1.03 fM. The limit of detection (LOD) was calculated according to LOD = 3 N/S. Besides, the sensor showed good reproducibility and stability, which present provided new method for early and highly sensitive detection for plant viruses.

show abstract

“…To address these limitations, cardiac biomarkers such as cardiac troponin I (CTNI), cardiac troponin T (CTNT), creatine kinase-MB, and myoglobin have been developed for the diagnosis of AMI [6,7]. Among these biomarkers, CTNI is considered the "gold standard" for the diagnosis of acute myocardial infarction [8,9] because CTNI levels change with heart muscle cell damage, but not in patients with skeletal muscle diseases. In normal healthy individuals, the level of CTNI is usually less than 0.2ng/ml.…”

Section: Introductionmentioning

confidence: 99%

Magnetically controlled graphene field-effect transistor biosensor for highly sensitive detection of cardiac troponin I

Zhu

Cheng

Ding

et al. 2023

Preprint

View full text Add to dashboard Cite

Herein, we have constructed a magnetic graphene field-effect transistor biosensor for highly sensitive detection of cardiac troponin I (CTNI). Graphene film was used as a conductive channel and transferred onto a glass substrate with two indium tin oxide (ITO) electrodes. CTNI aptamer was immobilized onto the graphene film via 1-pyrene-butanoic acid succinimidyl ester (PBASE) to capture CTNI. Magnetic nanobeads modified with CTNI antibody were added to the reaction chamber to form an aptamer/CTNI/antibody/magnetic nanobeads sandwich-type complex. By applying a periodic magnetic field, we found that the magnetic force exerted on the complex leads to an impedance change of the graphene film. The results were rationalized using a model wherein the magnetic force causes the CTNI aptamer strand to bend, thereby resulting in magnetic nanobeads/CTNI aptamer modulation of the double conductive layer of graphene transistors. With periodic sampling integration, different concentrations of CTNI can be detected with high sensitivity. Due to the specific binding property of the aptamer to the antigen, the magnetic graphene field-effect transistor biosensor can be used for the specific detection of different kinds of proteins and can potentially be a detection tool for DNA, specific cells, etc.

show abstract

Electrochemical immunosensor for highly sensitive detection of cTnI via in-situ initiated ROP signal amplification strategy

Cited by 17 publications

References 38 publications

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

A highly sensitive fluorescence sensor for tobacco mosaic virus RNA based on DSN cycle and ARGET ATRP double signal amplification

Magnetically controlled graphene field-effect transistor biosensor for highly sensitive detection of cardiac troponin I

Contact Info

Product

Resources

About