Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites

Chen, Xia; Jia, Xinle; Han, Jingman; Ma, Jie; Ma, Zhanfang

doi:10.1016/j.bios.2013.06.054

Cited by 190 publications

(66 citation statements)

References 42 publications

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“…DPV is applied to detect the electrochemical response peak of the two probes, before and after the formation of the two immunocomplexes. 0.5-60 ng mL -1 for both CEA and AFP (0.1 ng mL -1 for CEA and 0.05 ng mL -1 for AFP) (Chen et al, 2013) The results obtained from clinical serum sample are in agreement with ELISA and there is no cross-talk between the two target biomarkers.…”

Section: References and Other Remarkssupporting

confidence: 73%

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Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Luong

Vashist

2017

Biosensors and Bioelectronics

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Section: References and Other Remarkssupporting

confidence: 73%

“…Indeed, this approach has been developed for the detection of CEA and alpha-fetoprotein as discussed in Table 2 (Chen et al, 2013). Finally, there is also a need to devise and validate prospective multiplexing strategies, enabling the precise determination of CEA in a sample together with such biomarkers.…”

Section: Immunosensing Procedures For Ceamentioning

confidence: 99%

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Luong

Vashist

2017

Biosensors and Bioelectronics

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“…The authors synthesized CGS-PB and CGS-TB nanocomposites from commercial CGS (obtained from JCNANO, Nanjing, China). DPV responses of these electroactive labels were used for the sensitive and selective electrochemical detection providing LODs of 0.05 and 0.1 ng·mL −1 for AFP and CEA determination, respectively [60]. Similarly, Jia et al [61] developed another configuration for the same markers.…”

Section: Graphene Used As Carrier For Signal Elements In Electrochemimentioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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“…This indicates great potential for label-free biosensors. For this reason, while the methods based on fluorescence, such as enzyme-linked immunosorbent assay (ELISA), are still the most popular method to detect specific proteins in today's medicine in terms of detection of tumor biomarkers, label-free biosensors based on electrochemical immunoassay have attracted great attention due to their simple installation, low-cost, and easy operation [17,18]. The sensor in this paper demonstrates the detection of alpha-fetoproteins (AFP), one of the most important biomarkers associated with liver cancer and ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

2017

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Combination of shrink induced nano-composites technique and layer-by-layer (LbL) self-assembled graphene challenges controlling surface morphology. Adjusting shrink temperature achieves tunability on graphene surface morphology on shape memory polymers, and it promises to be an alternative in fields of highsurface-area conductors and molecular detection. In this study, self-assembled graphene on a shrink polymer substrate exhibits nanowrinkles after heating. Induced nanowrinkles on graphene with different shrink temperature shows distinct surface roughness and wettability. As a result, it becomes more hydrophilic with higher shrink temperatures. The tunable wettability promises to be utilized in, for example, microfluidic devices. The graphene on shrink polymer also exhibits capability of being used in sensing applications for pH and alphafetoprotein (AFP) detection with advantages of label free and low cost, due to self-assembly technique, easy functionalization, and antigen-antibody reaction on graphene surface. The detection limit of AFP detection is down to 1 pg/mL, and therefore the sensor also has a significant potential for biosensing as it relies on low-cost self-assembly and label-free assay.

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Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites

Cited by 190 publications

References 42 publications

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Shrink-induced graphene sensor for alpha-fetoprotein detection with low-cost self-assembly and label-free assay

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