Electrochemical immunosensor using nanotriplex of graphene quantum dots, Fe3O4, and Ag nanoparticles for tuberculosis

Tufa, Lemma Teshome; Oh, Sang-Jin; Tran, Van Tan; Kim, Jeonghyo; Jeong, Ki‐Jae; Park, Tae Jung; Kim, Hwa Jung; Lee, Jaebeom

doi:10.1016/j.electacta.2018.09.108

Cited by 75 publications

(37 citation statements)

References 54 publications

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“…By using amperometry in the presence of H 2 O 2 , the immunosensor provided a wide dynamic range (5 fg mL −1 –50 ng mL −1 ), a low LOD (2 fg mL −1 ), and could be used for the analysis of spiked human serum samples. Tufa et al [57] proposed an electrochemical sandwich immunosensor for the detection of Mycobacterium tuberculosis antigen (culture filtrate protein, CFP-10) using a nanotriplex consisting of a GQD-coated Fe 3 O 4 @Ag core-shell nanostructure (Fe 3 O 4 @Ag/GQD) as a the GCE modifier and AbD-AuNPs as labels for signal amplification (Figure 3). The sensing platform using the nanotriplex material showed a noticeable synergetic electrochemical performance due to the different roles of the three nanomaterials: Fe 3 O 4 increased the surface-to-volume ratio; Ag enhanced electrical conductivity; and GQDs allowed larger CAb loadings onto the electrode.…”

Section: Gqds and Cds In Electrochemical Biosensingmentioning

confidence: 99%

“…Synthesis of Fe 3 O 4 @Ag/GQD NP nanotriplex probe ( a ) and outline of inmmunosensor preparation ( b ): drop casting of the nanotriplex probe onto the GCE (Step 1), incubation with CAb (Step 2), blocking with BSA (Step 3), incubation with the target antigen (Step 4), and incubation with the AbD-AuNPs (Step 5). Reprinted with permission from [57]. Copyright Elsevier, 2018.…”

Section: Figurementioning

confidence: 99%

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Carbon Dots and Graphene Quantum Dots in Electrochemical Biosensing

2019

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Graphene quantum dots (GQDs) and carbon dots (CDs) are among the latest research frontiers in carbon-based nanomaterials. They provide interesting attributes to current electrochemical biosensing due to their intrinsic low toxicity, high solubility in many solvents, excellent electronic properties, robust chemical inertness, large specific surface area, abundant edge sites for functionalization, great biocompatibility, low cost, and versatility, as well as their ability for modification with attractive surface chemistries and other modifiers/nanomaterials. In this review article, the use of GQDs and CDs as signal tags or electrode surface modifiers to develop electrochemical biosensing strategies is critically discussed through the consideration of representative approaches reported in the last five years. The advantages and disadvantages arising from the use of GQDs and CDs in this context are outlined together with the still required work to fulfil the characteristics needed to achieve suitable electrochemical enzymatic and affinity biosensors with applications in the real world.

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Section: Gqds and Cds In Electrochemical Biosensingmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Carbon Dots and Graphene Quantum Dots in Electrochemical Biosensing

2019

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“…Tufa and colleagues proposed a sandwich-immunoassay sensor to quantify Mycobacterium tuberculosis antigen (i.e., CFP-10) using AuNPs and GQD-embedded Fe 3 O 4(core) @Ag (shell) nanocomplex as signal amplification labels and GCE enhancer, respectively [288]. This immunosensor exhibited an additive effect due to the multifarious role played by the nanomaterials residing within the nanocomplex, i.e., Ag accelerated the electron transferability, GQDs increased the surface area for the effective immobilization of anti-CFP-10 antibodies, and Fe 3 O 4 enlarged the surface/volume ratio.…”

Section: Voltammetric Gqd-sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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“…The resultant Fe 3 O 4 @Au@Ag/GQDs were attracted to a GCE to immobilize an anti-CFP-10 primary antibody and bind specifically the CFP-10 antigen, while the AuNPs-Ab 2 nanobioconjugate was added to the electrode to complete the biosensing platform. The immunosensor signal response was interrogated by DPV, reaching a linear range from 0.005 to 500 µg/mL, with a LOD of 0.33 ng/mL [ 45 ].…”

Section: Nanobioconjugates Assemblymentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

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Electrochemical immunosensor using nanotriplex of graphene quantum dots, Fe3O4, and Ag nanoparticles for tuberculosis

Cited by 75 publications

References 54 publications

Carbon Dots and Graphene Quantum Dots in Electrochemical Biosensing

Carbon Dots and Graphene Quantum Dots in Electrochemical Biosensing

Applications of Graphene Quantum Dots in Biomedical Sensors

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

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