Electrochemical immunosensors and their recent nanomaterial-based signal amplification strategies: a review

Lim, Syazana Abdullah; Ahmed, Minhaz Uddin

doi:10.1039/c6ra00333h

Cited by 182 publications

(81 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 Electrochemical techniques are being increasingly used in the fabrication and design of PADs due to the potential for miniaturization, low sensor fabrication cost, compatibility with advanced microfabrication technology and high sensitivity that is made possible by better conductivity. 11 The electrochemical reading on paper substrate is highly comparable to results produced by the more conventional benchtop techniques, such as UV-Vis spectroscopy, liquid chromatography and inductively coupled plasma-mass spectrometry, in terms of sensitivity and specificity.…”

Section: Introductionsupporting

confidence: 51%

Trends in Paper-based Electrochemical Biosensors: From Design to Application

et al. 2018

Self Cite

View full text Add to dashboard Cite

Electrochemical bio-sensing using paper-based detection systems is the main focus of this review. The different existing designs of 2-dimensional and 3-dimensional sensors, and fabrication techniques are discussed. This review highlights the effect of adopting different sensor designs, distinct fabrication techniques, as well as different modification methods, in order to produce reliable and reproducible reading. The use of various nanomaterials have been demonstrated in order to modify the surface of electrodes during fabrication to further enhance the signal for subsequent analysis. The reviewed sensors were classified into categories based on their applications, such as diagnostics, environmental and food testing. One of the major advantages of using paper-based electrochemical sensors is the potential for miniaturization, which only requires relatively small amount of samples, and the low cost for the purpose of mass production. Additionally, most of the devices reviewed were made to be portable, making them well-suited for on-site detection. Finally, paper-based detection is an ideal platform to fabricate cost-effective, user-friendly and sensitive electrochemical biosensors, with large capacity for customization depending on functional needs.

show abstract

Section: Introductionsupporting

confidence: 51%

Trends in Paper-based Electrochemical Biosensors: From Design to Application

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The integration of nanomaterials in the biosensors has lead to significant improvements in signal transduction as they exhibit unique electronic, physical and chemical properties [23,24]. Graphene, the recent member of the carbon nanomaterials family, have shown interesting applications in many biosensing applications [25].…”

Section: Introductionmentioning

confidence: 99%

Competitive voltammetric morphine immunosensor using a gold nanoparticle decorated graphene electrode

Eissa

Zourob

2017

Microchim Acta

View full text Add to dashboard Cite

The authors describe a voltammetric immunosensor for morphine (MOR) that is based on a graphene screen printed electrode (GSPE) modified with gold nanoparticles (AuNP) which were electrodeposited on its surface. The gold nanoparticles were characterized by scanning electron microscopy, Xray photoelectron spectroscopy and cyclic voltammetry. Cysteamine was then self-assembled on the gold-modified electrodes via thiol interaction in order to introduce terminal amino groups to the electrode surface. The electrodes were then used to fabricate the immunosensor by covalent immobilization of antibodies against MOR. The sensor works on the basis of a competition between MOR and the morphine-bovine serum albumin (MOR-BSA) conjugate for the immobilized antibodies on the sensor surface and the resulting change in the square wave voltammetry reduction current using the hexacyanoferrate system as an electrochemical probe. The competitive immunoassay enables sensitive and selective detection of MOR in the 0.1 to 100 ng·mL −1 MOR concentration range, with a 90 pg·mL −1 detection limit. The method was applied to the determination of MOR in spiked saliva samples and showed high recoveries. We believe that this immunosensor offers a simple, rapid, sensitive and accurate MOR test.

show abstract

“…Affinity sensors rely on a selective binding interaction between the analyte and a biological component such as an antibody, nucleic acid or a receptor [1]. Nano-materials enable to provide unique chemical and physical properties such as high surface-to-volume ratio and unique conductivity, enabling advanced functions for the enhancement of detectable signal [9]. The interaction between bioreceptor and biomolecule is measured by the biotransducer which outputs a measurable signal proportional to the presence of the target analyte in the sample.…”

Section: Introductionmentioning

confidence: 99%

“…Undoubted, nanotechnology provides new signal amplification strategies in various transducer platforms to achieve high sensitivity and low detection limit [8]. Nano-materials enable to provide unique chemical and physical properties such as high surface-to-volume ratio and unique conductivity, enabling advanced functions for the enhancement of detectable signal [9]. Furthermore, functional nanomaterials can not only produce a synergic effect among catalytic activity, conductivity, and biocompatibility to accelerate the signal transduction but also amplify biorecognition events with specifically designed signal tags, leading to highly sensitive biosensing.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

Alizadeh

Salimi

2018

Electroanalysis

View full text Add to dashboard Cite

This article reviews recent advances and developments in the field of bioaffinity electrochemical sensors with emphasis on a subclass including immunosensors, aptasensors, genosensors and molecularly imprinted sensosr. Recent insights into novel fabrication methodologies and electrochemical techniques have resulted in the demonstration of biosensors able to detection of wide range of target analyte. It has been shown that due to their high specificity and sensitivity, they represent a great tool in practical applications. Furthermore, at last couple decades nanotechnology has become very popular in the sensor fields due to the high capacity of nanomaterials to immobilize of bioaffinity agents and also their inhibiting effects on denaturation of enzymes and other bioaffinity systems, which they could well have beneficial effects for the performance of these sensors. The main focus of this review is to discuss methods for immobilizing bioreceptors onto a solid support, introduction of nanomaterials for improve sensitivity of biosensor and various electrochemical sensing platforms. The use of biosensor in these applications is most appropriate, because the analysis of trace substances in medical and healthcare applications, environmental science, and food industries is so important. In addition, recent advances in nanomaterial‐mediated bioaffinity sensors used for onset biosensing, point‐of‐care testing and healthcare management are also highlighted.

show abstract

Electrochemical immunosensors and their recent nanomaterial-based signal amplification strategies: a review

Abstract: In recent years, tremendous advances have been made in biosensors based on nanoscale electrochemical immunosensors for use in the fields of agriculture, food safety, biomedicine, quality control, and environmental and industrial monitoring.

Cited by 182 publications

References 115 publications

Trends in Paper-based Electrochemical Biosensors: From Design to Application

Trends in Paper-based Electrochemical Biosensors: From Design to Application

Competitive voltammetric morphine immunosensor using a gold nanoparticle decorated graphene electrode

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

Contact Info

Product

Resources

About