Electrochemical Impedance Biosensor for the Determination of Lipopolysaccharide Using Peptide as the Recognition Molecule

Abstract

A simple and sensitive electrochemical impedance spectroscopy (EIS) biosensor was designed for the determination of lipopolysaccharide (LPS). The gold nanoparticles (GNPs) were electrochemically deposited on the glassy carbon electrode as a base electrode and the biosensor was fabricated by self-assembling a special peptide (GIGKLHSAGKF) used as molecular recognition onto the GNPs surface according to the Au-S bond. Polyethylene glycol and 6-mercapto-1-hexanol were used to block the remaining sites of the modified electrode surface. When binding of LPS, the biosensor had a good response to different concentrations of LPS and the charge transfer resistance of the biosensor was directly related to the logarithm of LPS concentrations in the range of 0.01 pg mL -1 -1.0 ng mL -1 with a limit of detection of 2.0 fg mL -1 . The characteristics of the electrochemically deposited GNPs and the optimization concentration and the self-assembly time of peptide, as well as the performance of the EIS biosensors for LPS were investigated. The biosensor was used for real serum sample determination with satisfactory results.

Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria

“…This is in agreement with previously published reports where immobilized cationic peptides or proteins have been used for electrostatic recognition and ultimate electrochemical detection of LPS [11,[16][17][18]20]. Moreover, the enhancement in the electrochemical signal intensity over increasing LPS concentrations is also in good agreement with previous published reports [43,44]. Overall, the sensor performance in terms of LOD and dynamic range is comparable to, or exceeds, the reported values (Table 1).…”

Highly sensitive and selective acute myocardial infarction detection using aptamer‐tethered MoS 2 nanoflower and screen‐printed electrodes

“…Electrochemical impedance spectroscopy is effective for precisely measuring the charge transfer processes within electrochemical sensors [13,14]. EIS analysis was carried out to quantify the electrochemistry of MoS 2 NFs and MoS 2 NSs before being immobilized and hybridized on the SPE electrode.…”

Environmental protection based on the nanobiosensing of bacterial lipopolysaccharides (LPSs): material and method overview

“…8). 145 A dual electrochemical and mechanical label free lectin platform was developed for highly sensitive detection (LOD ¼ up to 50 cells per mL) of antimicrobial susceptibility and the quantication of bacterial LPS. In this method, a polythiophene interface containing glycosylated fusion quinone units is used to form a carbohydrate platform for immobilization of concanavalin A (Con A), and LPS binding is measured via an orthogonal crystal oscillator microbalance and perform an electrochemical read (EQCM).…”

“…8 Graphic illustration of the label-free EIS biosensor for LPS detection. 145 DNAzyme-assisted recycling and Ce-based metal-organic structures dual signal ampliers for sensitive electrochemical detection of LPS were settled. In the planned biosensor, Ce-MOFs were decorated with AuNPs to achieve AuNPs/Ce-MOFs.…”