Electrochemical impedance of randomly distributed defects in tethered phospholipid bilayers: Finite element analysis

Raila, Tomas; Penkauskas, Tadas; Jankunec, Marija; Dreižas, Gintaras; Meškauskas, Tadas; Valinčius, Gintaras

doi:10.1016/j.electacta.2018.12.148

Cited by 21 publications

(28 citation statements)

References 30 publications

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“…Both attraction and LCN models can produce distributions with matching values, however, the attraction model depends more on the overall features of real defect distribution (defect size and density), which might be difficult to estimate or measure experimentally. As noted in earlier work [ 7 ], radiuses of membrane defects (introduced by VLY) usually fall in range from 13 nm to 25 nm. This fact might be useful in estimating attraction model parameters based on summary statistics ( and others).…”

Section: Methodsmentioning

confidence: 82%

“…Each defect distribution consisted of 500 defects. Defect densities and sizes for attraction model were selected by likely scenarios examined in the earlier study [ 7 ]. Other values were chosen arbitrarily, to cover a wide range of visually different clustering cases.…”

Section: Methodsmentioning

confidence: 99%

“…The basic defect distribution type, used in the current and previous [ 7 ] study is the random defect distribution (Fig. 1 ), in which defect center coordinates (X and Y) are sampled from uniform probability distribution, independently for each defect.…”

Section: Defect Distribution Modelsmentioning

confidence: 99%

“…The statistical distributions of sector areas obtained from computing such diagrams are characterized in terms of four summary statistics: standard deviation ( ), skewness, kurtosis and median absolute deviation (MAD). All studied defect distributions are bounded by hexagonal shape of the modeling domain in the same way as the membrane models solved with FEA technique in earlier work [ 7 ].

Fig.…”

Section: Defect Distribution Modelsmentioning

confidence: 99%

“…One particular study in this specific topic dealt with synthetic EIS spectra computed for 3D membrane models using finite-element analysis (FEA) [ 6 , 7 ]. EIS spectral features were used to estimate basic properties of membrane defects, such as their size and density.…”

Section: Introductionmentioning

confidence: 99%

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Computational Models of Defect Clustering for Tethered Bilayer Membranes

Raila

Jankunec

Meškauskas

et al. 2020

Computational Science and Its Applications – ICCSA 2020

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This study deals with computational modeling of defect clustering effects observed in bilayer phospholipid membranes. Two defect clustering models (algorithms) are presented and compared with the random defect distribution approach. Specific defect distribution instances are evaluated using a simple methodology based on Voronoi diagrams and statistical properties of their sector areas. Computational experiments are performed by using the models to generate synthetic defect distributions with different parameter combinations. The proposed methodology is also validated against atomic force microscopy images of real membranes with defects.

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Section: Methodsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Defect Distribution Modelsmentioning

confidence: 99%

Fig.…”

Section: Defect Distribution Modelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Computational Models of Defect Clustering for Tethered Bilayer Membranes

Raila

Jankunec

Meškauskas

et al. 2020

Computational Science and Its Applications – ICCSA 2020

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Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes

Penkauskas

Valinčius

2021

Methods in Molecular Biology

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In this paper, we describe the application of electrochemical impedance spectroscopy (EIS) to characterize process of formation and properties of solid-supported tethered bilayer membranes on solid conducting substrates. Along with the description of experimental procedures to prepare substrates and self-assembly of phospholipid bilayers onto gold-coated glass slides, we describe the detailed protocols of EIS measurements. We demonstrate the utility of EIS in the evaluation of the properties of both molecular anchor layers used to immobilize tBLMs as well as characterization of tBLMs. We show that the EIS methodology extends the applicability of this technique well beyond the mere evaluation of electric parameters. Specifically, we demonstrate how by using EIS one may evaluate both density and size of water-filled defects (ion-channels) in tBLMs, to determine the structural mode (homogeneous, heterogeneous, or clustered) of distribution of defects in tBLMs. Our methodology can be applied in both basic protein membrane interaction studies, as well as in the development of precision biosensoric systems with tBLMs as a sensing element.

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