Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles

Chen, Zhengbo; Li, Lidong; Zhao, Hang; Guo, Lin; Mu, Xiaojiao

doi:10.1016/j.talanta.2010.11.042

Cited by 75 publications

(38 citation statements)

References 26 publications

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“…The selectivity of the electrochemical aptasensors was proven by challenging them with excess quantities of other proteins (e.g., bovine serum albumin, thrombin, immunoglobulin G, hemoglobin, myoglobin, casein, cytochrome C) tested either in individual solutions or in mixtures with lysozyme [44,46,61,74,76,77,79,82,84,[89][90][91]. Some authors went further and proved also the selectivity of the lysozyme aptamer sequence by performing measurements with an identical sensor in which the aptamer was replaced by a scrambled DNA sequence [81,82,87].…”

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

“…Moreover, most aptasensors reported satisfactory storage stability with less than a 16% decrease in the analytical signal at 4˝C over 2 weeks-1 month [44,47,74,76,91]. While in some cases, sensor development targeted single-use [63], there were a few aptasensors for which multiple regeneration cycles were performed without affecting the magnitude of the analytical signal (less than 10% decrease for 3-5 regenerations) [46,62,89,90].…”

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

“…To increase the aptamer loading, a popular approach relies on modifying the electrodes with Au nanoparticles [47,76].…”

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confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

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Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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“…Especially, electrochemical impedance spectroscopy (EIS) has been proven to be a most powerful and sensitive tool for probing the features of surface-modified electrodes [21]. And EIS biosensors ([Fe(CN) 6 ] 3-/4-as redox probe couple) possess unique advantages, such as the ability to separate the surface binding events from the solution impedance, ease of signal quantification, less damage to the biological interactions being measured, and most importantly, label-free on the DNA strand [22][23][24]. However, to the best of our knowledge, little efforts have been given to the development of biosensors for exploring the 2-HOFlu, and no fluorescent, colorimetric and electrochemical DNA sensors have been reported for 2-HOFlu detection.…”

Section: Introductionmentioning

confidence: 99%

G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic

Liang

Liu

2015

Microchim Acta

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We describe a sensitive and selective biosensor for the environmental metabolite 2-hydroxyfluorene (2-HOFlu). It is based on electrochemical impedance spectroscopy and was obtained by assembling a thiolated single-stranded DNA on a gold electrode via S-Au covalent bonding. It is then transformed to a K + -stabilized G-quadruplex-hemin complex which exhibits peroxidase-like activity to catalyze the oxidation of 2-HOFlu by H 2 O 2 . This results in the formation of insoluble products that are precipitated on the gold electrode. As a result, the charge transfer resistance (R CT ) between the solution and the electrode surface is strongly increased within 10 min as demonstrated by using the ferro/ferricyanide system as a redox probe. The difference in the charge transfer resistances (ΔR CT ) before and after incubation of the DNA film with 2-HOFlu and H 2 O 2 serves as the signal for the quantitation of 2-HOFlu with a 1.2. nM detection limit in water of pH 7.4. The assay is highly selective over other selected fluorene derivatives. It was exploited to determine 2-HOFlu in spiked lake water samples where it displayed a detection limit of 3.6 nM. Conceivably, this method has a wide scope in that it may be applied to other analytes for which respective G-quadruplexes are available.

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“…It is chemically synthesized using simpler and more cost-effective procedures, with higher stability and possibility of performing favorable modifications. [17][18][19][20][21] Aptamers are oligonucleotides that are able to bind to their targets with specificity and affinity comparable to monoclonal antibodies, because of the specific three-dimensional structures that they form according to their sequences. 21 In accordance with the aforementioned issues, a targeted polymeric nanoparticulate system composed of poly(butylene adipate-co-butylene terephthalate) (Ecoflex ® ; BASF Company, Ludwigshafen, Germany) and HER-2 specific aptamer was investigated in the current study, and its properties such as in vitro cytotoxicity against a HER-2-positive ovarian cancer, pharmacokinetic profile, and in vivo antitumor efficacy in tumor-bearing nude mice were evaluated.…”

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confidence: 99%

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer

Ghassami¹,

Varshosaz²,

Jahanian-Najafabadi³

et al. 2018

IJN

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Purpose Epithelixal ovarian cancer is the fourth cause of cancer death in developed countries with 77% of ovarian cancer cases diagnosed with regional or distant metastasis, with poor survival rates. Docetaxel (DTX) is a well-known anticancer agent, with clinically proven efficacy in several malignancies, including ovarian cancer. However, the adverse effects caused by the active ingredient or currently marketed formulations could even deprive the patient of the advantages of treatment. Therefore, in the current study, polymeric nanoparticles (NPs) equipped with aptamer molecules as targeting agents were proposed to minimize the adverse effects and enhance the antitumor efficacy through directing the drug cargo toward its site of action. Materials and methods Electrospraying technique was implemented to fabricate poly (butylene adipate-co-butylene terephthalate) (Ecoflex ® ) NPs loaded with DTX (DTX-NPs). Afterward, aptamer molecules were added to the DTX-NPs, which bound via covalent bonds (Apt-DTX-NPs). The particle size, size distribution, zeta potential, entrapment efficiency, and release profile of the NPs were characterized. Using MTT assay and flow-cytometry analysis, the in vitro cytotoxicity and cellular uptake of the NPs were compared to those of the free drug. Following intravenous administration of Taxotere ® , DTX-NPs, and Apt-DTX-NPs (at an equivalent dose of 5 mg/kg of DTX), pharmacokinetic parameters and antitumor efficacy were compared in female Balb/c and HER-2-overexpressing tumor-bearing B6 athymic mice, respectively. Results The obtained results demonstrated significantly enhanced in vitro cytotoxicity and cellular uptake of Apt-DTX-NPs in a HER-2-overexpressing cell line, comparing to DTX-NPs and the free drug. The results of in vivo studies indicated significant increment in pharmacokinetic parameters including the area under the plasma concentration–time curve, mean residence time, and elimination half-life. Significant increment in antitumor efficacy was also observed, probably due to the targeted delivery of DTX to the tumor site and enhanced cellular uptake as evaluated in the aforementioned tests. Conclusion Hence, the proposed drug delivery system could be considered as an appropriate potential substitute for currently marketed DTX formulations.

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Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles

Cited by 75 publications

References 26 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer

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Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles

Cited by 75 publications

References 26 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic

Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>&reg;</sup> nanoparticles for docetaxel delivery in ovarian cancer

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Pharmacokinetics and in vitro/in vivo antitumor efficacy of aptamer-targeted Ecoflex<sup>®</sup> nanoparticles for docetaxel delivery in ovarian cancer