Electrochemical impedance spectroscopy for study of aptamer–thrombin interfacial interactions

Li, Xiaoxia; Shen, Linyong; Zhang, Dongdong; Qi, Honglan; Gao, Qiang; Ma, Fen; Zhang, Chengxiao

doi:10.1016/j.bios.2008.01.029

Cited by 152 publications

(68 citation statements)

References 38 publications

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“…The rapid evolution of the symptoms requires a rapid, sensitive and reliable monitoring of bacterial contamination. Biosensors can assume this role in this case [4,5], which in combination with electrochemical techniques for transduction of protein recognition can provide the simplicity and speed required.…”

Section: Introductionmentioning

confidence: 99%

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Queirós

de‐los‐Santos‐Álvarez

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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a b s t r a c tA label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs) was developed. Two single stranded DNA sequences were tested as recognition elements and compared. The aptamer capture probes were immobilized, with and without 6-mercapto-1-hexanol (MCH) on a gold electrode. Each step of the modification process was characterized by Faradaic impedance spectroscopy (FIS). A linear relationship between the electron-transfer resistance (R et ) and E. coli OMPs concentration was demonstrated in a dynamic detection range of 1 × 10 −7 -2 × 10 −6 M. Moreover, the aptasensor showed selectivity despite the presence of other possible water contaminates and could be regenerated under low pH condition. The developed biosensor shows great potential to be incorporated in a biochip and used for in situ detection of E. coli OMPs in water samples.

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Section: Introductionmentioning

confidence: 99%

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Queirós

de‐los‐Santos‐Álvarez

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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“…The majority of structure-switching G4 electrochemical aptasensors were developed using gold electrodes, although, more recently, other electrochemical transducers have been employed, such as gold disk microelectrode arrays [70], modified platinum [71] or carbon electrodes [63,[71][72][73][74][75].…”

Section: Structure-switching G4 Electrochemical Aptasensormentioning

confidence: 99%

“…Among them, ferrocene [72,[83][84][85][86][87][88][89] and Ru(NH 3 ) 6 ] 3+ [75,90] were very popular, especially for the construction of impedimetric aptasensors.…”

Section: Structure-switching G4 Electrochemical Aptasensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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“…26 The ECL biosensor was fabricated by drop-coating 20 μL of 10 μM Ru1-peptide solution onto the surface of the desired GNPsmodified SPCEs and incubating for 1 h at room temperature. And then the resulting electrode was washed with the washing buffer and was subsequently blocked with 1.0 mM MCH for 30 min.…”

Section: Fabrication Of Ecl Biosensor and Bioassay Of Mmp-7mentioning

confidence: 99%

Design and Application of Multi-functional Electrogenerated Chemiluminescence Imaging Analyzer

et al. 2016

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A multi-functional eletrogenerated chemiluminescence (ECL) imaging analyzer including both a photomultiplier tube and charged coupled device as detectors has been developed. The ECL imaging analyzer can effectively work for electrochemical study, ECL intensity detection at electrode array, and ECL imaging at bipolar electrodes or electrode array. As an ECL imaging example, an ECL biosensor for visual detection of matrix metalloproteinase 7 in the range from 0.05 to 1 ng/mL is demonstrated.

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Electrochemical impedance spectroscopy for study of aptamer–thrombin interfacial interactions

Cited by 152 publications

References 38 publications

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Guanine Quadruplex Electrochemical Aptasensors

Design and Application of Multi-functional Electrogenerated Chemiluminescence Imaging Analyzer

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