Electrochemical indirect competitive immunoassay for ultrasensitive detection of zearalenone based on a glassy carbon electrode modified with carboxylated multi-walled carbon nanotubes and chitosan

Xu, Wei; Qing, Ying; Chen, Shuai; Chen, Jun; Qin, Zhen; Qiu, Jingfu; Li, ChaoRui

doi:10.1007/s00604-017-2342-9

Cited by 49 publications

(12 citation statements)

References 31 publications

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Section: Resultsmentioning

confidence: 99%

“…The sensitivity of the aptasensor for ZEN detection was significantly enhanced compared with that of most of other analytical techniques which are listed in Table S1. ,,, Also, the designed aptasensor had a wider linear range relative to the most of these approaches. Among these analytical techniques, the electrochemical indirect competitive immunoassay showed better LOD and linear range compared to our aptasensor. However, this method needed 3.5 h analysis time and two kinds of antibodies, leading to the increase of the cost of analytical method, whereas the analysis time of our aptasensor was less than 2 h and the sensing agent was aptamer, leading to the decrease of the cost of sensing platform.…”

Section: Resultsmentioning

confidence: 99%

“…Zearalenone (ZEN), a kind of resorcylic acid lactone, is a secondary metabolite produced by several Fusarium species. , ZEN has low acute toxicity in most species, and its LD 50 in rats and chickens is more than 2 g/kg body weight . However, ZEN can be found in most of grain crops, including rice and wheat .…”

Section: Introductionmentioning

confidence: 99%

“…However, ZEN can be found in most of grain crops, including rice and wheat . Chronic exposure to ZEN can cause severe adverse effects, such as neurotoxicity, carcinogenicity, and immunotoxicity. , Also, ZEN as a mycoestrogen can impair reproduction. , Therefore, innovation in the design of analytical techniques for the detection of ZEN is vitally important.…”

Section: Introductionmentioning

confidence: 99%

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Novel Colorimetric Aptasensor for Zearalenone Detection Based on Nontarget-Induced Aptamer Walker, Gold Nanoparticles, and Exonuclease-Assisted Recycling Amplification

Taghdisi

Danesh²,

Ramezani

et al. 2018

ACS Appl. Mater. Interfaces

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Zearalenone (ZEN) toxicity is a significant risk for human beings. Thus, it is of high importance to develop sensitive, precise, and inexpensive analytical methods for ZEN detection, especially in human serum. Here, a colorimetric aptasensor is presented for the determination of ZEN based on the nontarget-induced aptamer walker, catalytic reaction of gold nanoparticles (AuNPs), exonuclease III (Exo III) as a signal amplifier, and 4-nitrophenol as a colorimetric agent. Low amount of ZEN requirement and signal amplification are some of the distinct advantages of the proposed aptasensor. In the absence of ZEN, the aptamer (Apt) starts walking on the AuNP surface with the help of Exo III and binds to multiple complementary strands of aptamer, leading to the change of sample color from yellow to colorless. Upon the addition of ZEN, both the Apt and complementary strand exist as single-stranded DNAs on the surface of AuNPs, resulting in less access of 4-nitrophenol to the surface of AuNPs and less catalytic performance of AuNPs. In this situation, the color of the sample remains yellow (the color of 4-nitrophenol). The presented aptasensor was capable to detect ZEN in a wide linear dynamic range, 20-80 000 ng/L, with a detection limit of 10 ng/L. The prepared sensing strategy was successfully used for ZEN determination in the human serum sample.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Colorimetric Aptasensor for Zearalenone Detection Based on Nontarget-Induced Aptamer Walker, Gold Nanoparticles, and Exonuclease-Assisted Recycling Amplification

Taghdisi

Danesh²,

Ramezani

et al. 2018

ACS Appl. Mater. Interfaces

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“…In recent years, immunoassay techniques, which are based on the highly specific molecular recognition of antigens by antibodies, have become an important analytical method in several fields [21,22]. Furthermore, many electrochemical immunosensors for food safety analysis were developed, due to the electrochemical methods provides several useful properties including the required detection limit, fast analysis times, low cost of the instrumentation, low sample and reagent consumption, portability, ease of use, high sensitivity and selectivity [23,24]. Screen-printing electrodes is one of the most promising approaches towards simple, rapid and inexpensive immunosensors production, and has advantages of flexibility design, automation process, and good reproducibility [25].…”

Section: A C C E P T E D Mmentioning

confidence: 99%

Mesoporous immunosensor applied to zearalenone determination in Amaranthus cruentus seeds

Regiart

Fernández

Vicario

et al. 2018

Microchemical Journal

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We describe an electrochemical immunosensor for zearalenone (ZEA) determination in Amaranthus cruentus seeds by an enzyme immunoassay sandwich type. The device is based on a screen-printed carbon electrode (SPCE) modified with amino mesoporous silica (MCM-41) synthetized with Fe2O3 in situ. Mesoporous material enlarges the surface available for anti-ZEA antibodies immobilization. SPCE/MCM-41-Fe2O3 was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry.ZEA in the sample previously pretreated was recognized and captured by anti-ZEA on SPCE/MCM-41-Fe2O3. Then, the horseradish peroxidase (HRP)-conjugated anti-ZEAantibody was added, and the substrate solution (H2O2 + 4-terbutylcatechol (4-TBC)) reacted with the HRP that catalyzed the oxidation of 4-TBC to 4-terbutylbenzoquinone (TBQ).Finally, the enzymatic product was detected at _ 100 mV, and the current was proportional to the ZEA present in the sample.The calibration plot exhibited a linear range from 1.88 to 45 ng mL -1 , and the limit of detection was 0.57 ng mL -1 (r 2 = 0.998). The coefficient of variation inter-and intra assay was below 6%. Our method achieved a good selectivity, stability and reproducibility for ZEA detection in A. cruentus seeds.

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Nanotechnology‐based electrochemical biosensors for monitoring breast cancer biomarkers

Nasrollahpour

Khalilzadeh

Hasanzadeh

et al. 2022

Medicinal Research Reviews

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Breast cancer is categorized as the most widespread cancer type among women globally. On-time diagnosis can decrease the mortality rate by making the right decision in the therapy procedure. These features lead to a reduction in medication time and socioeconomic burden. The current review article provides a comprehensive assessment for breast cancer diagnosis using nanomaterials and related technologies. Growing use of the nano/biotechnology domain in terms of electrochemical nanobiosensor designing was discussed in detail. In this regard, recent advances in nanomaterial applied for amplified biosensing methodologies were assessed for breast cancer diagnosis by focusing on the advantages and disadvantages of these approaches. We also monitored designing methods, advantages, and the necessity of suitable (nano) materials from a statistical standpoint.The main objective of this review is to classify the applicable biosensors based on breast cancer biomarkers.With numerous nano-sized platforms published for breast cancer diagnosis, this review tried to collect the most suitable methodologies for detecting biomarkers and certain breast cancer cell types.

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Electrochemical indirect competitive immunoassay for ultrasensitive detection of zearalenone based on a glassy carbon electrode modified with carboxylated multi-walled carbon nanotubes and chitosan

Cited by 49 publications

References 31 publications

Novel Colorimetric Aptasensor for Zearalenone Detection Based on Nontarget-Induced Aptamer Walker, Gold Nanoparticles, and Exonuclease-Assisted Recycling Amplification

Novel Colorimetric Aptasensor for Zearalenone Detection Based on Nontarget-Induced Aptamer Walker, Gold Nanoparticles, and Exonuclease-Assisted Recycling Amplification

Mesoporous immunosensor applied to zearalenone determination in Amaranthus cruentus seeds

Nanotechnology‐based electrochemical biosensors for monitoring breast cancer biomarkers

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