Electrochemical investigation of an interaction of the antidepressant drug aripiprazole with original and damaged calf thymus dsDNA

Yu²,

et al. 2016

Luminescence

In this study, the interaction between a novel ruthenium(II) arene complex with curcumin analogs and calf thymus DNA (ctDNA) was investigated systematically by viscosity measurement, the DNA melting approach, multispectroscopic techniques and electrochemical methods. The absorption spectra of the ctDNA-drug complex showed a slight red shift and a weak hypochromic effect. The relative viscosity and melting temperature of ctDNA increased on addition of the drug. The evidence obtained from fluorescence competitive experiments indicated that the binding mode of the drug with ctDNA was intercalative. Using acridine orange (AO) as a fluorescence probe, the drug statically quenched the fluorescence of the ctDNA-AO complex, and hydrogen bonding and van der Waals interactions played vital roles in the binding interaction between the drug and ctDNA. The influences of ionic strength, chemical denaturants and pH on the binding interaction were also investigated. Circular dichroism and Fourier transform infrared spectra suggested that this drug might bond with the G-C base pairs of ctDNA and the right-handed B-form helicity of ctDNA remained after drug binding. The intercalative binding between the drug and ctDNA was further investigated using electrochemical techniques. All these results suggested that the biological activity of ctDNA was affected by ruthenium(II) arene complex with curcumin analogs. Copyright © 2016 John Wiley & Sons, Ltd.

Section: Resultsmentioning

confidence: 99%

Systematical investigation of binding interaction between novel ruthenium(II) arene complex with curcumin analogs and ctDNA

Yu²,

et al. 2016

Luminescence

“…In voltammetric techniques, the measured electrochemical signal is an algebraic sum of the undesirable capacitive current and the desired current related to the proper electrode reaction, the so-called Faraday current. DPV is an electrochemical technique which is widely used in chemical analysis and for studying the interactions of pharmaceuticals with DNA [ 41 , 43 , 45 , 51 , 60 , 63 , 65 , 94 , 95 ]. Its high sensitivity results from the pulsating change of potential applied to the working electrode.…”

Section: Electrochemical Methods Appliedmentioning

confidence: 99%

“…Consequently, the impact of the subsequent portions (increasing the DNA concentration) on the redox processes of the examined system is determined [ 24 , 26 , 44 , 46 , 59 , 63 ]. An alternative method is the modification of the electrode, which involves immobilizing one of the analyzed system elements (studied compound with biological activity [ 64 ] or DNA [ 41 , 42 , 57 , 61 , 62 , 63 , 65 , 66 ]) on the surface of the working electrode. The last-mentioned approach is often used to obtain DNA biosensor systems for monitoring drug interactions.…”

Section: Electrochemical Approach To Dna–drug Interaction Descriptionmentioning

confidence: 99%

What Can Electrochemical Methods Offer in Determining DNA–Drug Interactions?

2021

The interactions of compounds with DNA have been studied since the recognition of the role of nucleic acid in organisms. The design of molecules which specifically interact with DNA sequences allows for the control of the gene expression. Determining the type and strength of such interaction is an indispensable element of pharmaceutical studies. Cognition of the therapeutic action mechanisms is particularly important for designing new drugs. Owing to their sensitivity, simplicity, and low costs, electrochemical methods are increasingly used for this type of research. Compared to other techniques, they require a small number of samples and are characterized by a high reliability. These methods can provide information about the type of interaction and the binding strength, as well as the damage caused by biologically active molecules targeting the cellular DNA. This review paper summarizes the various electrochemical approaches used for the study of the interactions between pharmaceuticals and DNA. The main focus is on the papers from the last decade, with particular attention on the voltammetric techniques. The most preferred experimental approaches, the electrode materials and the new methods of modification are presented. The data on the detection ranges, the binding modes and the binding constant values of pharmaceuticals are summarized. Both the importance of the presented research and the importance of future prospects are discussed.

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

“…Ancak DNA-ilaç mekanizmasının araştırıldığı elektrokimyasal çalışmalarda, yaygın olarak guanin ve adenin yükseltgenme sinyallerinden yararlanılır [17]. Guanin ve adenin yükseltgenme sinyallerinin belirgin bir şekilde gözlenmesini sağlayan puls voltametrik teknikler, ekstraksiyon veya ön-işlem gereksinimi olmadan analiz imkânı sağlaması, kısa analiz süresine ve yüksek duyarlığa sahip olması gibi üstünlükleri nedeniyle ilaç-DNA etkileşimi çalışmalarında oldukça sık kullanılmaktadır [18][19][20].…”

Section: Introductionunclassified

Development of Electrochemical DNA Sensor Based on Poly (Bromocresol purple) Modified Glassy Carbon Electrode for the Determination of 2-Thiouracil

Zeybek

Doğanci

Karaşalli

2020

Bu çalışmada, potansiyel bir kanser ilacı olan 2-tiyourasil (2-TU) ilacı ile balık sperminden elde edilen çift zincirli deoksiribonükleik asit (dsDNA) molekülü arasındaki etkileşim incelenmiş ve bu etkileşime dayalı olarak ilacın elektrokimyasal tayini gerçekleştirilmiştir. Bunun için, camsı karbon elektrot (GCE) yüzeyi, bromokrezol moru (BCP) monomerinin elektrokimyasal polimerizasyonu ile modifiye edilmiş ve bu elektrot (GCE/P(BCP)) yüzeyine, dsDNA elektrokimyasal olarak immobilize edilmiştir (GCE/P(BCP)/dsDNA). dsDNA ile 2-TU arasındaki etkileşim mekanizması diferansiyel puls voltametri yöntemiyle araştırılmıştır. Bu etkileşim sonrası guaninin yükseltgenme pik akımında azalma gözlenmiş ve bu azalmaya bağlı olarak 2-TU'in elektrokimyasal tayini indirekt yöntemle gerçekleştirilmiştir. 2-TU için doğrusal çalışma aralığı 0,1-50 mg L −1 ve gözlenebilme sınırı 0,033 mg L −1 olarak bulunmuştur. 2-TU−dsDNA etkileşim mekanizması UV-Görünür bölge moleküler absorpsiyon spektroskopi yöntemiyle de incelenmiştir. Hazırlanan DNA biyosensörüne bozucu etki yapabilecek türlerin etkisi araştırılmış ve ayrıca 2-TU ilacının idrar numunesinde tayini gerçekleştirilmiştir. Deneysel çalışmalardan elde edilen sonuçlara göre, 2-TU ve dsDNA arasındaki başlıca etkileşim modunun interkalasyon olduğu belirlenmiştir.