Electrochemical investigations into Tau protein phosphorylations

Martić, Sanela; Beheshti, Samaneh; Rains, Meghan K.; Kraatz, Heinz‐Bernhard

doi:10.1039/c2an35097a

Cited by 40 publications

(31 citation statements)

References 39 publications

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“…1,2 However, in many applications, these sensors are exposed to complex biological media along with the analyte of interest. The accumulation of biological components such as proteins, lipids, and polysaccharides on the sensor surface is referred to as biofouling.…”

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confidence: 99%

Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

et al. 2015

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Electrochemical nucleic acid sensors are promising tools for point-of-care diagnostic platforms with their facile integration with electronics and scalability. However, nucleic acid detection in complex biological fluids is challenging as biomolecules nonspecifically adsorb on the electrode surface and adversely affect the sensor performance by obscuring the transport of analytes and redox species to the electrode. We report that nanoporous gold (np-Au) electrodes, prepared by a microfabrication-compatible self-assembly process and functionalized with DNA probes, enabled detection of target DNA molecules (10–200 nM) in physiologically relevant complex media (bovine serum albumin and fetal bovine serum). In contrast, the sensor performance was compromised for planar gold electrodes in the same conditions. Hybridization efficiency decreased by 10% for np-Au with coarser pores revealing a pore-size dependence of sensor performance in biofouling conditions. This nanostructure-dependent functionality in complex media suggests that the pores with the optimal size and geometry act as sieves for blocking the biomolecules from inhibiting the surfaces within the porous volume while allowing the transport of nucleic acid analytes and redox molecules.

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confidence: 99%

Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

et al. 2015

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“…Ferrocene (Fc) is a typical sandwich metallocene compound, which contains a couple of planar cyclopentadienyl anion ring with 6π electrons and 6d electrons on Fe (II) [20,21]. It's remarkable that the introduction of Fc molecule on GO not only can draw into further function [22], but also can significantly increase the dispersibility and specific surface area of GO [23]. As a well-known intermediate mediator in electrochemical field, Fc shows excellent electrochemical proper-ties, including fast electronetransfer rate, low oxidation potential and stability of two redox states [24,25], drawing extensive attention in functionalization GO due to its excellent electronic transmission ability [26,27].…”

Section: Introductionmentioning

confidence: 99%

The Synthesis of Chitosan Decorated Reduced Graphene Oxide‐Ferrocene Nanocomposite and its Application in Electrochemical Detection Rhodamine B

Xing

Niu

et al. 2019

Electroanalysis

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Chitosan (CS) decorated reduced graphene oxide‐Ferrocene nanocomposite (RGO−Fc−CS) was synthesized by a one‐pot reaction. In the synthesis process of RGO−Fc−CS, GO−Fc was successfully self‐assembled via the π–π interaction, which had the features of large surface area and the high load. Then GO‐Fc and CS linked via one‐pot under alkaline conditions. The FT−IR, TGA, SEM were employed to characterize the successful synthesis of RGO−Fc−CS composites. A miniature electrochemical system was fabricated by RGO−Fc−CS modified glassy carbon electrode (expressed as RGO−Fc−CS/GCE) for the sensitive detection of RhB enantiomers via DPV. Electrochemical results revealed that the RGO−Fc−CS/GCE exhibited high molecular recognition toward RhB. Due to the synergy between the RGO−Fc and CS, the RGO−Fc−CS/GCE showed a linear range of 0.001–70 μM with a LOD of 0.5 nM, indicating that RGO−Fc−CS/GCE has broad application prospects to simplify monitor RhB real‐time.

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“…Clinical studies suggested that AD brain and traumatic brain injury exhibit similarities between accumulation of amyloid peptides mainly beta-amyloid (β-A) and tau protein (Tsitsopoulos and Marklund 2013). To monitor AD in real samples such as interstitial fluid (ISF) and cerebrospinal fluid (CSF), both tau protein and β-A have served as potential biomarkers (Kolarova et al 2012; Martić et al 2012a; Martić et al 2012b; Martic et al 2013; Rains et al 2013; Tsitsopoulos and Marklund 2013). To make presented review well-focused and comprehensive, we highlight exclusively β-A as a potential biomarker for AD detection using nano-enabling biosensors.…”

Section: Introduction: Alzheimer’s Diseases and Challengesmentioning

confidence: 99%

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Kaushik

Jayant

Tiwari

et al. 2016

Biosensors and Bioelectronics

154

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Beta-amyloid (β-A) peptides are potential biomarkers to monitor Alzheimer’s diseases (AD) for diagnostic purposes. Increased β-A level is neurotoxic and induces oxidative stress in brain resulting in neurodegeneration and causes dementia. As of now, no sensitive and inexpensive method is available for β-A detection under physiological and pathological conditions. Although, available methods such as neuroimaging, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) detect β-A, but they are not yet extended at point-of-care (POC) due to sophisticated equipments, need of high expertise, complicated operations, and challenge of low detection limit. Recently, β-A antibody based electrochemical immuno-sensing approach has been explored to detect β-A at pM levels within 30–40 minutes compared to 6–8 hours of ELISA test. The introduction of nano-enabling electrochemical sensing technology could enable rapid detection of β-A at POC and may facilitate fast personalized health care delivery. This review explores recent advancements in nano-enabling electrochemical β-A sensing technologies towards POC application to AD management. These analytical tools can serve as an analytical tool for AD management program to obtain bio-informatics needed to optimize therapeutics for neurodegenerative diseases diagnosis management

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Electrochemical investigations into Tau protein phosphorylations

Cited by 40 publications

References 39 publications

Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

Biofouling-Resilient Nanoporous Gold Electrodes for DNA Sensing

The Synthesis of Chitosan Decorated Reduced Graphene Oxide‐Ferrocene Nanocomposite and its Application in Electrochemical Detection Rhodamine B

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

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