Electrochemical magneto immunosensor based on endogenous β-galactosidase enzyme to determine enterotoxicogenic Escherichia coli F4 (K88) in swine feces using square wave voltammetry

Tarditto, Lorena Viviana; Zón, Marı́a Alicia; Ovando, Hugo García; Vettorazzi, N.; Arévalo, Fernando Javier; Fernández, Héctor

doi:10.1016/j.talanta.2017.06.059

Cited by 19 publications

(4 citation statements)

References 33 publications

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“…There are several pathotypes of E. coli causing enteritis (enterotoxigenic (ETEC); vero- or Shiga-like toxin producing (VTEC or STEC); nacrotoxigenic (NTEC); enteropathogenic (EPEC); enterohaemorrhagic (EHEC); enteroaggregative (EAggEC); and enteroinvasive (EIEC)) with different pathogenicity, epidemiological, and clinical courses [ 35 ]. Specifically, ETEC serotypes produce the highest rates of morbidity and mortality during neonatal and post-weaning periods [ 36 , 37 , 38 , 39 ].…”

Section: Acquired Specific Piglet Immunity Through the Sowmentioning

confidence: 99%

Maternal Vaccination. Immunization of Sows during Pregnancy against ETEC Infections

et al. 2017

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The immunology of pregnancy is an evolving consequence of multiple reciprocal interactions between the maternal and the fetal-placental systems. The immune response must warrant the pregnancy outcome (including tolerance to paternal antigens), but at the same time, efficiently respond to pathogenic challenges. Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in neonatal and recently weaned pigs. This review aims to give an overview of the current rationale on the maternal vaccination strategies for the protection of the newborn pig against ETEC. Newborn piglets are immunodeficient and naturally dependent on the maternal immunity transferred by colostrum for protection—a maternal immunity that can be obtained by vaccinating the sow during pregnancy. Our current knowledge of the interactions between the pathogen strategies, virulence factors, and the host immune system is aiding the better design of vaccination strategies in this particular and challenging host status. Challenges include the need for better induction of immunity at the mucosal level with the appropriate use of adjuvants, able to induce the most appropriate and long-lasting protective immune response. These include nanoparticle-based adjuvants for oral immunization. Experiences can be extrapolated to other species, including humans.

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Section: Acquired Specific Piglet Immunity Through the Sowmentioning

confidence: 99%

Maternal Vaccination. Immunization of Sows during Pregnancy against ETEC Infections

et al. 2017

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“…Enzymes, antibodies, cells or tissues, which possess high biological activity, can be immobilized at the surface of the transducer using various procedures. These include entrapment behind a membrane, entrapment within a polymeric matrix, entrapment within self-assembled monolayers (SAMs), covalent bonding on surfaces or membranes activated by bifunctional groups or spacers, and bulk modification of the entire electrode material [33,34,52,[54][55][56]60,[64][65][66][67][68][69][70]. Electrochemical biosensors commonly used are of three types of recognition elements: (1) enzymes, which are the most common and welldeveloped recognition system, and can be either mono-or multi-enzyme-based; (2) whole cells (microorganisms such as bacteria, fungi, eukaryotic cells, or yeast) or cell organelles or particles (such as mitochondria and cell walls); and (3) tissues (such as plant or animal tissue slices) [71].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Multivariate Optimization of Electrochemical Biosensors for the Determination of Compounds Related to Food Safety—A Review

et al. 2023

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We summarize the application of multivariate optimization for the construction of electrochemical biosensors. The introduction provides an overview of electrochemical biosensing, which is classified into catalytic-based and affinity-based biosensors, and discusses the most recent published works in each category. We then explore the relevance of electrochemical biosensors for food safety analysis, taking into account analytes of different natures. Then, we describe the chemometrics tools used in the construction of electrochemical sensors/biosensors and provide examples from the literature. Finally, we carefully discuss the construction of electrochemical biosensors based on design of experiments, including the advantages, disadvantages, and future perspectives of using multivariate optimization in this field. The discussion section offers a comprehensive analysis of these topics.

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“…Although a lower LOD was found via CV measurement, colorimetric measurements that have an acceptable resolution are more beneficial for potential POC applications. Besides CV, other electric techniques such as differential pulse voltammetry (DPV) [ 47 ] and square wave voltammetry (SWV) [ 48 ] have also been widely used for protein immunosensing.…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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Electrochemical magneto immunosensor based on endogenous β-galactosidase enzyme to determine enterotoxicogenic Escherichia coli F4 (K88) in swine feces using square wave voltammetry

Cited by 19 publications

References 33 publications

Maternal Vaccination. Immunization of Sows during Pregnancy against ETEC Infections

Maternal Vaccination. Immunization of Sows during Pregnancy against ETEC Infections

Multivariate Optimization of Electrochemical Biosensors for the Determination of Compounds Related to Food Safety—A Review

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

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