Electrochemical measurement of antibody-antigen recognition biophysics: Thermodynamics and kinetics of human chorionic gonadotropin (hCG) binding to redox-tagged antibodies

Partington, Lee I.; Atkin, Stephen L.; Morris, Samuel H.; Piper, Mark; Lawrence, Nathan S.; Wadhawan, Jay D.

doi:10.1016/j.jelechem.2018.02.062

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…[26] linear range of 0.001-0.2 IU/mL with a LOD of 0.6 mIU/mL. The authors note that IU (international unit)/mL refers to the international arbitrary amount of a substance agreed upon by scientists and doctors; in the case of hCG, a reading of above 25 mIU/mL is considered a positive result for pregnancy [109], and a result <5 mIU/mL generally indicates a negative test [110]. Another interesting use of AuNPs was reported by Li et al [26] who created a hybrid array biosensor for the detection of penicillin and tetracycline using penicillinase and L-cysteine as biorecognition elements, respectively (Fig.…”

Section: Nanoparticle-based Biosensorsmentioning

confidence: 99%

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Crapnell

Banks

2021

Microchim Acta

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Research into electrochemical biosensors represents a significant portion of the large interdisciplinary field of biosensing. The drive to develop reliable, sensitive, and selective biosensing platforms for key environmental and medical biomarkers is ever expanding due to the current climate. This push for the detection of vital biomarkers at lower concentrations, with increased reliability, has necessitated the utilisation of micro- and nano-dimensional materials. There is a wide variety of nanomaterials available for exploration, all having unique sets of properties that help to enhance the performance of biosensors. In recent years, a large portion of research has focussed on combining these different materials to utilise the different properties in one sensor platform. This research has allowed biosensors to reach new levels of sensitivity, but we note that there is room for improvement in the reporting of this field. Numerous examples are published that report improvements in the biosensor performance through the mixing of multiple materials, but there is little discussion presented on why each nanomaterial is chosen and whether they synergise well together to warrant the inherent increase in production time and cost. Research into micro-nano materials is vital for the continued development of improved biosensing platforms, and further exploration into understanding their individual and synergistic properties will continue to push the area forward. It will continue to provide solutions for the global sensing requirements through the development of novel materials with beneficial properties, improved incorporation strategies for the materials, the combination of synergetic materials, and the reduction in cost of production of these nanomaterials. Graphical abstract

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Section: Nanoparticle-based Biosensorsmentioning

confidence: 99%

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Crapnell

Banks

2021

Microchim Acta

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“…A decrease of signal intensity of the oxidation of ferrocene label when incubated with specific antigen was observed. Very recently, the thermodynamics and kinetics of human chorionic gonadotropin (hCG) binding to the surface of electrodes modified with ferrocene-tagged antibodies theoretically and experimentally have been studied [54]. Upon the formation of antigen-antibody complex, the blocking of ferrocene voltammetry occurs.…”

Section: Direct Signal Immunosensors Based On Antibody-electroactive mentioning

confidence: 99%

Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

Kondzior

Grabowska

2020

Sensors

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Suitable immobilization of a biorecognition element, such as an antigen or antibody, on a transducer surface is essential for development of sensitive and analytically reliable immunosensors. In this review, we report on (1) methods of antibody prefunctionalization using electroactive probes, (2) methods for immobilization of such conjugates on the surfaces of electrodes in electrochemical immunosensor construction and (3) the use of antibody-electroactive probe conjugates as bioreceptors and sensor signal generators. We focus on different strategies of antibody functionalization using the redox active probes ferrocene (Fc), anthraquinone (AQ), thionine (Thi), cobalt(III) bipyridine (Co(bpy)33+), Ru(bpy)32+ and horseradish peroxidase (HRP). In addition, new possibilities for antibody functionalization based on bioconjugation techniques are presented. We discuss strategies of specific, quantitative antigen detection based on (i) a sandwich format and (ii) a direct signal generation scheme. Further, the integration of different nanomaterials in the construction of these immunosensors is presented. Lastly, we report the use of a redox probe strategy in multiplexed analyte detection.

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“…2. The scan rate was 10 mV s −1 to enable more time at a set potential which would kinetically favor the ferrocene oxidation (Partington et al 2018).…”

Section: Oxidation Of Ab-fc On the Surfacementioning

confidence: 99%

A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers

Abdulsattar

Greenway

2019

J Anal Sci Technol

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An electrochemically based antibody immobilization was used to perform environmentally and clinically relevant immunoassays for stress hormones biomarkers (cortisol and cortisone) using chemiluminescence (CL) detection. To achieve CL detection, the ferrocene tag on the antibodies was first oxidised, and this then acted as a catalyst for the luminol and hydrogen peroxide CL reaction. The conditions were optimised and measurements were made with an incubation time of 30 min. Using this approach limits of detection were obtained of 0.47 pg ml −1 and 0.34 pg ml −1 also R 2 0.9912 and 0.9902 for cortisol and cortisone respectively with a linear concentration from 0 to 50 ng ml −1. The method was then applied to Zebrafish whole body and artificial saliva samples. For the Zebrafish sample recoveries of 91.0% and 90.0% were obtained with samples spiked with cortisol and cortisone, for artificial saliva the recoveries were 92.59% and 90.73% respectively. Interference studies showed only minor effects on the measurement of the analyte. A comparison between this procedure and the standard enzyme-linked immunosorbent assay (ELISA) procedure gave approximately the same R 2 values.

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Electrochemical measurement of antibody-antigen recognition biophysics: Thermodynamics and kinetics of human chorionic gonadotropin (hCG) binding to redox-tagged antibodies

Cited by 8 publications

References 33 publications

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Electroanalytical overview: utilising micro- and nano-dimensional sized materials in electrochemical-based biosensing platforms

Antibody-Electroactive Probe Conjugates Based Electrochemical Immunosensors

A sensitive chemiluminescence based immunoassay for the detection of cortisol and cortisone as stress biomarkers

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