Electrochemical measurement of microbial activity

Ikeda, Hisafumi; Itagaki, Satohiro; Nishii, Shigeki; Yamamoto, Yasutake; Sadanaga, Yasuhiro; Shiigi, Hiroshi

doi:10.5189/revpolarography.68.15

Cited by 1 publication

(1 citation statement)

References 51 publications

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“…Bacteria grow and proliferate in four stages: lag, logarithmic, stationary, and death phases. 36) During the lag phase, the first phase of the bacterial growth cycle, bacteria take in medium components, activate their metabolism, and produce energy. The length of the lag phase is called lag time.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Antibiotic Penicillin G Activities Based on Electrochemical Measurement of a Tetrazolium Salt

Ikeda,

Tokonami,

Nishii

et al. 2024

CHEMICAL & PHARMACEUTICAL BULLETIN

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This study focused on the electrochemical properties of tetrazolium salts to develop a simple method for evaluating viable bacterial counts, which are indicators of drug susceptibility. Considering that the oxidized form of tetrazolium, which has excellent cell membrane permeability, changes to the insoluble reduced form formazan inside the cell, the number of viable cells was estimated based on the reduction current of the tetrazolium remaining in the bacterial suspension. Dissolved oxygen is an important component of bacterial activity. However, it interferes with the electrochemical response of tetrazolium. We estimated the number of viable bacteria in the suspension based on potential-selective current responses that were not affected by dissolved oxygen. Based on solubility, cell membrane permeability, and characteristic electrochemical properties of the tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium, we developed a method for rapidly measuring viable bacteria within one-fifth of the time required by conventional colorimetric methods for drug susceptibility testing.

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Section: Resultsmentioning

confidence: 99%