Electrochemical measurements of biofilm development using polypyrrole enhanced flexible sensors

Zheng, Leo Y.; Congdon, Robert; Sadik, Omowunmi A.; Marques, Cláudia N. H.; Davies, David G.; Sammakia, Bahgat; Lesperance, Leann M.; Turner, James N.

doi:10.1016/j.snb.2013.03.097

Cited by 28 publications

(22 citation statements)

References 29 publications

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“…Sensors, measuring physical value referred to as impedance, display promising properties that may be used for this purpose (Ben-Yoav et al, 2013;Zhenga et al, 2013;Padresa et al, 2013). Impedance describes the potential of natural objects to resist the electric current flow and to store energy in the form of electric or magnetic field.…”

Section: Introductionmentioning

confidence: 99%

The application of impedance microsensors for real-time analysis of Pseudomonas aeruginosa biofilm formation

Chabowski¹,

Junka²,

Szymczyk³

et al. 2015

Pol J Microbiol

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A b s t r a c tBiofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates the ability of impedance microsensors to detect Pseudomonas aeruginosa biofilm presence using the impedance spectroscopy method. The measured data were analyzed using Electrical Equivalent Circuit modelling (EEC). It allowed to recognize conduction and polarization phenomena on the sensors surface and in its environment. The impedance assay results, confirmed by means of electron microscopy and quantitative cultures, indicate that specific EEC parameters may be used for monitoring the development of pseudomonal biofilm. K e y w o r d s: Pseudomonas aeruginosa, biofilm, impedance sensors

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Section: Introductionmentioning

confidence: 99%

The application of impedance microsensors for real-time analysis of Pseudomonas aeruginosa biofilm formation

Chabowski¹,

Junka²,

Szymczyk³

et al. 2015

Pol J Microbiol

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“…Basing on the literature [9,14,28,29,32], the measured impedance spectra and knowledge about the physiochemical properties of biofilm [5,6,8,16], the electrical equivalent circuits (EEC) were obtained and are shown in Fig. 5.…”

Section: Electrical Equivalent Circuitsmentioning

confidence: 99%

“…As specific components of the EEC are correlated with various conduction and polarisation processes, such an approach enables to identify and separate them. This method is not widely used in analysis of the impedimetric sensors' responses in microbiological applications despite of the fact that it may provide detailed information about the measured object [14,17,28,29].…”

Section: Introductionmentioning

confidence: 99%

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Chabowski¹,

Junka²,

Piasecki³

et al. 2017

Metrology and Measurement Systems

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The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor's surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.

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“…One of the emerging methods for biofilm detection is the use of electrochemical impedance spectroscopy (EIS) measures by employing polymeric sensors. [12][13][14][15][16][17] In EIS, 4 the electrochemical impedance across the electrode-electrolyte interface is measured over a wide range of frequencies to elicit information about the properties of the interface. In the case of biofilms, successful detection is based on changes linked to charge transfer resistance and capacitance corresponding to the maturing stages of biofilm development.…”

Section: Introductionmentioning

confidence: 99%

Predicting Adsorption Energies Using Multifidelity Data

Tian

Rangarajan

2019

J. Chem. Theory Comput.

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Composite i-PP/PEDOT films made of isotactic polypropylene (i-PP), which is frequently used for the fabrication of implantable medical devices for internal use, and chemically synthesized poly(3,4-ethylendioxythiophene) (PEDOT) nanoparticles, which are electroactive and biocompatible, have been prepared and used to detect biofilm infection. After chemical and morphological characterization, the properties (interfacial, mechanical, thermal and electrochemical) and biocompatibility of i-PP/PEDOT have been examined. Besides, carbon screen-printed electrodes coated with i-PP/PEDOT have been found to detect the growth of Gram-positive and Gram-negative bacteria through the oxidation of nicotinamide adenine dinucleotide (NADH), which comes from the bacteria metabolism (i.e. the respiration). Thus, as outer bacterial membranes are permeable to cytosolic NADH, this metabolite has been found to be an appropriated target for the detection of growing bacterial infections (biofilms). In addition, the sensor does not respond towards eukaryotic cells. This is because the major NADH pool in eukaryotic cells is located at the mitochondria and, therefore, the concentration of in the medium is not high enough to be detected since the inner mitochondrial membrane is impermeable to NADH or NAD+.

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Electrochemical measurements of biofilm development using polypyrrole enhanced flexible sensors

Cited by 28 publications

References 29 publications

The application of impedance microsensors for real-time analysis of Pseudomonas aeruginosa biofilm formation

The application of impedance microsensors for real-time analysis of Pseudomonas aeruginosa biofilm formation

Impedance Sensors Made in PCB and LTCC Technologies for Monitoring Growth and Degradation of Pseudomonal Biofilm

Predicting Adsorption Energies Using Multifidelity Data

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