Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores

Navrátil, Tomáš; Šestáková, Ivana; Štulı́k, Karel; Mareček, Vladimı́r

doi:10.1002/elan.201000061

Cited by 19 publications

(18 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Asolectin from soybeans is a mixture of phospholipids extracted from soybeans with an approximate composition of 25% phosphatidylcholine, 25% cephalin, 25% phosphatidylinositol, and small amounts of other phospholipids from soybeans . Approximately 24% of the fatty acid chains in asolectin are saturated, 14% are mono‐unsaturated, and 62% are poly‐unsaturated . A generic chemical representation of asolectin from soybeans is provided in Figure (C).…”

Section: Introductionmentioning

confidence: 99%

Asolectin from soybeans as a natural compatibilizer for cellulose‐reinforced biocomposites from tung oil

Johns

Morris

Edwards

et al. 2015

J of Applied Polymer Sci

View full text Add to dashboard Cite

The free radical copolymerization of tung oil, divinylbenzene, and n-butyl methacrylate results in bio-based thermosetting polymers with tunable properties. Biocomposites have been obtained by the reinforcement of such bio-based resins with a-cellulose. Asolectin from soybeans consists of a mixture of natural, polyunsaturated phospholipids. Because of its long, unsaturated fatty acid chains, and the presence of phosphate and ammonium groups, asolectin from soybeans is a good candidate for acting as a natural compatibilizer between the hydrophobic matrix and the hydrophilic reinforcement. In the current work, we investigate the changes in properties resulting from the addition of asolectin to a tung oil-based polymer reinforced with a-cellulose. An evaluation of the curekinetics of the tung oil-based resin has been conducted by dielectric analysis (DEA), and the final biocomposites have been thoroughly characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA), Soxhlet extraction, and scanning electron microscopy (SEM).

show abstract

Section: Introductionmentioning

confidence: 99%

Asolectin from soybeans as a natural compatibilizer for cellulose‐reinforced biocomposites from tung oil

Johns

Morris

Edwards

et al. 2015

J of Applied Polymer Sci

View full text Add to dashboard Cite

show abstract

“…“Insert” cell, was used, which was described earlier . In this arrangement, the polycarbonate membrane was glued (epoxy resin CAS 25068‐38‐6 (UHU, Bühl, Germany)) onto the plastic cup with a small hole in the center (0.12 cm 2 ), prior to the application of the PL solution.…”

Section: Methodsmentioning

confidence: 99%

“…To elucidate transport processes, synthetically prepared model PL bilayers (PLBs) are utilized, in the form of black membranes, vesicles or supported membranes with incorporated ion channels .…”

Section: Introductionmentioning

confidence: 99%

“…For the possibility of application of voltammetry in study of transport of species across artificial PLM, two‐compartment electrochemical vessel was designed, where both compartments were separated by PLM on polycarbonate support . Formation and stability of PLM was followed with EIS.…”

Section: Introductionmentioning

confidence: 99%

“…For transport of cadmium ions, commercially available calcium ionophore A23187 (calcimycin) has been incorporated into PLM. Transport of bivalent cations in this case proceeds complex formation . For transport of phytochelatin PC 2 we firstly used cell‐penetrating peptides, where under special conditions transient pore is formed and some amount of species, which has to be present in excess, is simultaneously transported across PLM .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Employment of Voltammetry in Studies of Transport Processes across Artificial Phospholipid Membranes

2016

Self Cite

View full text Add to dashboard Cite

This paper reports on voltammetric methods applied in studies of transport processes connected with the occurrence of toxic cadmium in plants. As a means of transport across model of phospholipid membrane (PLM), calcium ionophore (calcimycin), cell‐penetrating peptides (transportan 10 and mastoparan), and finally protoplasts isolated from barley roots and leaves were used. Using calcimycin, different influences of Cd complexation by citric acid or by malic acid as examples of root exudates were documented. For transport of simplest phytochelatin PC2, voltammetric methods using formation of Hg complex on hanging mercury drop electrode (HMDE) or of Ag complex on mercury meniscus covered silver solid amalgam electrode (m‐AgSAE) were assessed.

show abstract

Model Biological Membranes and Possibilities of Application of Electrochemical Impedance Spectroscopy for their Characterization

et al. 2017

Self Cite

View full text Add to dashboard Cite

Biological membranes are essential parts of living systems. They represent an interface between intracellular and extracellular space. Depending on their structure, they often perform very complex functions and play an important role in the transport of both charged and uncharged particles in any organism. Structure of the biological membranes, which play very important role in electrochemical processes inside living organisms, is very complicated and still not precisely defined and explained. Model lipid membranes are used to gain detail information about properties of real biological membranes and about associated electrochemical processes. Electrochemistry, especially electrochemical impedance spectroscopy (EIS), can play a useful role in the characterization of properties of model lipid membranes (planar and supported lipid bilayers, tethered lipid membranes, liposomes, etc.). This review is focused on model biological membranes and the possibilities and limitations of electrochemical methods and namely of EIS in this field.

show abstract

Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores

Cited by 19 publications

References 37 publications

Asolectin from soybeans as a natural compatibilizer for cellulose‐reinforced biocomposites from tung oil

Asolectin from soybeans as a natural compatibilizer for cellulose‐reinforced biocomposites from tung oil

Employment of Voltammetry in Studies of Transport Processes across Artificial Phospholipid Membranes

Model Biological Membranes and Possibilities of Application of Electrochemical Impedance Spectroscopy for their Characterization

Contact Info

Product

Resources

About