Electrochemical microfluidic biosensor for nucleic acid detection with integrated minipotentiostat

Kwakye, Sylvia; Goral, Vasily N.; Baeumner, Antje J.

doi:10.1016/j.bios.2005.11.017

Cited by 110 publications

(74 citation statements)

References 27 publications

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“…This straightforward extension to an electrochemical system is advantageous as electrochemical detection methods offer several benefits over popularly used optical detection techniques. These include low capital cost for equipment, portability, low power requirement, and lack of photobleaching issues (Kwakye et al 2006;Kwakye and Baeumner 2007).…”

Section: Discussionmentioning

confidence: 99%

Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli

Connelly

Baeumner

et al. 2011

Microfluid Nanofluid

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We present a microfluidic biosensor that integrates membrane-based preconcentration with fluorescence detection. The concentration membrane was fabricated in polyacrylamide by an in situ photopolymerization technique at the junction of glass microchannels. Liposomes entrapping sulforhodamine B dye molecules were used for signal amplification. The biotin-streptavidin binding system was a model system for evaluating device performance. Biotinylated liposomes were preconcentrated at the membrane by applying an electric field across the membrane. The electric field causes the liposomes to migrate toward the membrane where they are concentrated by a sieving effect. Two orders of magnitude concentration was achieved after applying the electric field for only 2 min. The concentrated bolus was then eluted toward the detection unit, where the biotinylated liposomes were captured by immobilized streptavidin. The integrated system with the preconcentration module shows a 14-fold improvement in signal as opposed to a system that does not include preconcentration.

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Section: Discussionmentioning

confidence: 99%

Integrated microfluidic preconcentrator and immunobiosensor

Kondapalli

Connelly

Baeumner

et al. 2011

Microfluid Nanofluid

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“…Methods such as detachment lithography [117] and alterations to the photoresist spin step [118] have been shown to resolve this issue. This method is frequently used in micro/nanofluidic-based biosensors to fabricate fluidic channels [6,20,127] and is also used in the fabrication of silicon nanowires [24,95]. Typical feature sizes are in the several micrometres range with the smallest features of the order of 2-5 mm.…”

Section: (B) Fabrication Techniques and Methodsmentioning

confidence: 99%

“…Other target molecules include micro-RNA (miRNA) [24,28], a biomarker for cancer, DNA [15,20,25,30,34,61,122], herpes simplex-2 virus infection [5] and common drinking water contaminants including E. coli [45], pesticides [22,35], synthetic oestrogen in river water [21] and heavy metals [3,[162][163][164].…”

Section: Applicationsmentioning

confidence: 99%

Theory, fabrication and applications of microfluidic and nanofluidic biosensors

Prakash

Pinti

Bhushan

2012

Phil. Trans. R. Soc. A.

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Biosensors are a broad array of devices that detect the type and amount of a biological species or biomolecule. Several different types of biosensors have been developed that rely on changes to mechanical, chemical or electrical properties of the transduction or sensing element to induce a measurable signal. Often, a biosensor will integrate several functions or unit operations such as sample extraction, manipulation and detection on a single platform. This review begins with an overview of the current state-of-the-art biosensor field. Next, the review delves into a special class of biosensors that rely on microfluidics and nanofluidics by presenting the underlying theory, fabrication and several examples and applications of microfluidic and nanofluidic sensors.

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“…Therefore the binding capacity of the microdevice without porous silicon is approximately 75ng/cm 2 which agrees with the results of Cady et al (Cady et al, 2003) who found that the binding capacity of nonporous micropillars was approximate 82ng/cm 2 . The previous researches proved that the performance of DNA extraction microdevice was determined by the surface area of the matrix and the extracted DNA was found to increase linearly with the surface area (Fan et al, 1999;Kwakye et al, 2006). The binding capacity of porous silicon matrix would increase hundreds or thousands of times, while the extraction efficiency didn't improve so much.…”

Section: Optimization Of Cell Lysis Processmentioning

confidence: 99%

“…Micromachined analytical systems have several advantages over their large-scale counterparts, including low cost, disposability, low reagent and sample consumption, portability, and lower consumption. Many such devices have been demonstrated in the literature, including PCR microchips (Northrup et al, 1993;Copp et al, 1998;Panaro et al, 2005), DNA microchips (Fan et al, 1999), DNA biosensors (Kwakye et al, 2006), capillary electrophoresis (CE) microchips (Harrison et al, 1993;Backhouse et al, 2003;Liu et al, 2006), protein microchips (Yang et al, 2001;Wilson & Nie, 2006), etc. Most of these analytical processes need an effective yet simple method of obtaining high-quality DNA.…”

Section: Introductionmentioning

confidence: 99%