Electrochemical nano-genosensor for highly sensitive detection of miR-21 biomarker based on SWCNT-grafted dendritic Au nanostructure for early detection of prostate cancer

Sabahi, Abbas; Salahandish, Razieh; Ghaffarinejad, Ali; Omidinia, Eskandar

doi:10.1016/j.talanta.2019.120595

Cited by 83 publications

(34 citation statements)

References 72 publications

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“…The second most common cancer diagnosed worldwide is prostate cancer, which is the most common solid tumor in men resulting in the second leading cancer-related deaths (Culp et al, 2020;Sabahi et al, 2020). The death cases in 2018 from 359,000 is projected to increase up to 740,000 cases in 2040, with a rise of new cases from 1,276,000 in 2018 to 2.3 million in 2040, because of increased numbers of the aging population (Culp et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development, Optimization and Evaluation of 2-Methoxy-Estradiol Loaded Nanocarrier for Prostate Cancer

et al. 2021

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The therapeutic efficacy of antineoplastic agents possessing a selective target to the nucleus of the cancer cells could be enhanced through novel formulation approaches. Thus, toward the improvement of the anticancer potential of 2-methoxy estradiol (2 ME) on prostate cancer, the drug was entrapped into the hydrophobic micelles core formulated with Phospholipon 90G and d-α-tocopheryl polyethylene glycol succinate (TPGS). Optimization of the formulation was done by Box-Behnken statistical design using Statgraphics software to standardize percentages of TPGS and phospholipid to obtain the smallest particle size. The optimized formulation was found to be spherical with nanometer size of 152 ± 5.2 nm, and low PDI (0.234). The entrapment efficiency of the micelles was 88.67 ± 3.21% with >93% release of 2 ME within 24 h. There was a 16-fold increase in apoptosis and an 8-fold increase in necrosis of the PC-3 cells when incubated with 2 ME micellar delivery compared to control cells (2.8 ± 0.2%). This increased apoptosis was further correlated with increased BAX expression (11.6 ± 0.7) and decreased BCL-2 expression (0.29 ± 0.05) in 2 ME micelles treated cells when compared to the control group. Further, loss of mitochondrial membrane potential (∼50-fold) by the drug-loaded micelles and free drug compared to control cells was found to be due to the generation of ROS. Findings on cell cycle analysis revealed the significant arrest of the G2-M phase of the PC-3 cells when incubated with the optimized formulation. Simultaneously, a significantly increased number of cells in pre-G1 revealed the maximum apoptotic potential of the drug when delivered via micellar formulation. Finally, upregulation of caspase-9, p53, and NO, with downregulation of TNF-α, NF-κβ, and inflammatory mediators of the PC-3 cells established the superiority of the micellar approach against prostate cancer. In summary, the acquired results highlighted the potentiality of the 2 ME-micellar delivery tool for controlling the growth of prostate cancer cells for improved efficacy.

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Section: Introductionmentioning

confidence: 99%

Development, Optimization and Evaluation of 2-Methoxy-Estradiol Loaded Nanocarrier for Prostate Cancer

et al. 2021

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“…Gold nanoparticles (AuNPs) are often employed in biosensing platform due to the excellent catalytic and conductive properties. Sabahi et al (2020) modified bare fluorine doped tin oxide (FTO) electrode with single-walled carbon nanotube (SWCNT) and dendritic gold nanostructure for the detection of miR-21 with Cadmium ions (Cd 2+ ) as signal units as illustrated in Figure 3 [52]. Low detection limit (0.01 fM) was achieved and the fabricated biosensor also displayed acceptable performance in human serum samples.…”

Section: Detection Of Mir-21mentioning

confidence: 99%

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

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Chai

et al. 2021

Micromachines

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MicroRNAs (miRNAs) are important non-coding, single-stranded RNAs possessing crucial regulating roles in human body. Therefore, miRNAs have received extensive attention from various disciplines as the aberrant expression of miRNAs are tightly related to different types of diseases. Furthermore, the exceptional stability of miRNAs has presented them as biomarker with high specificity and sensitivity. However, small size, high sequence similarity, low abundance of miRNAs impose difficulty in their detection. Hence, it is of utmost importance to develop accurate and sensitive method for miRNA biosensing. Electrochemical biosensors have been demonstrated as promising solution for miRNA detection as they are highly sensitive, facile, and low-cost with ease of miniaturization. The incorporation of nanomaterials to electrochemical biosensor offers excellent prospects for converting biological recognition events to electronic signal for the development of biosensing platform with desired sensing properties due to their unique properties. This review introduces the signal amplification strategies employed in miRNA electrochemical biosensor and presents the feasibility of different strategies. The recent advances in nanomaterial-based electrochemical biosensor for the detection of miRNA were also discussed and summarized based on different types of miRNAs, opening new approaches in biological analysis and early disease diagnosis. Lastly, the challenges and future prospects are discussed.

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“…This method needs a labeled miRNA, which provides a hard step for the biosensor application in real samples. A labeled miRNA biosensor was recently described by Sabahi et al [193] for the quantification of miRNA-21 at fM level. This was obtained by the use of cadmium ions (Cd 2+ ) which is linked to a phosphate group of miRNA via an electrostatic reaction.…”

Section: Labeled Micrornamentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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Electrochemical nano-genosensor for highly sensitive detection of miR-21 biomarker based on SWCNT-grafted dendritic Au nanostructure for early detection of prostate cancer

Cited by 83 publications

References 72 publications

Development, Optimization and Evaluation of 2-Methoxy-Estradiol Loaded Nanocarrier for Prostate Cancer

Development, Optimization and Evaluation of 2-Methoxy-Estradiol Loaded Nanocarrier for Prostate Cancer

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

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