Electrochemical nanobiosensors equipped with peptides: a review

Negahdary, Masoud; Angnes, Lúcio

doi:10.1007/s00604-022-05184-x

Cited by 20 publications

(7 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peptides can be obtained using chemical or biological synthesis, as well as using combinatorial chemistry or phage display [223,224]. Peptides can be modified with various functional groups such as thiols, amines, carboxyls, biotin, streptavidin, etc., as well as various electroactive labels [225] to ensure specific and stable binding to the electrode [226]. Peptides can be targeted on the electrode surface using various strategies, such as the use of spatial probes, multivalent ligands, selective receptors, etc., to ensure optimal exposure of binding sites [227].…”

Section: Peptide "Aptamers"mentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

View full text Add to dashboard Cite

Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

show abstract

Section: Peptide "Aptamers"mentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

View full text Add to dashboard Cite

show abstract

“…The conversion of this signal can be achieved using different methods, and this can be classified according to the type of conversion used [80]. Finally, the signal conversion section can include the following optical systems: [69] electrochemical nanobiosensors [81], thermoelectric [82], and piezoelectric [83].…”

Section: Introductionmentioning

confidence: 99%

Emerging Applications of Nanobiosensors in Pathogen Detection in Water and Food

Valenzuela-Amaro,

Aguayo-Acosta,

Meléndez-Sánchez

et al. 2023

Biosensors

View full text Add to dashboard Cite

Food and waterborne illnesses are still a major concern in health and food safety areas. Every year, almost 0.42 million and 2.2 million deaths related to food and waterborne illness are reported worldwide, respectively. In foodborne pathogens, bacteria such as Salmonella, Shiga-toxin producer Escherichia coli, Campylobacter, and Listeria monocytogenes are considered to be high-concern pathogens. High-concern waterborne pathogens are Vibrio cholerae, leptospirosis, Schistosoma mansoni, and Schistosima japonicum, among others. Despite the major efforts of food and water quality control to monitor the presence of these pathogens of concern in these kinds of sources, foodborne and waterborne illness occurrence is still high globally. For these reasons, the development of novel and faster pathogen-detection methods applicable to real-time surveillance strategies are required. Methods based on biosensor devices have emerged as novel tools for faster detection of food and water pathogens, in contrast to traditional methods that are usually time-consuming and are unsuitable for large-scale monitoring. Biosensor devices can be summarized as devices that use biochemical reactions with a biorecognition section (isolated enzymes, antibodies, tissues, genetic materials, or aptamers) to detect pathogens. In most cases, biosensors are based on the correlation of electrical, thermal, or optical signals in the presence of pathogen biomarkers. The application of nano and molecular technologies allows the identification of pathogens in a faster and high-sensibility manner, at extremely low-pathogen concentrations. In fact, the integration of gold, silver, iron, and magnetic nanoparticles (NP) in biosensors has demonstrated an improvement in their detection functionality. The present review summarizes the principal application of nanomaterials and biosensor-based devices for the detection of pathogens in food and water samples. Additionally, it highlights the improvement of biosensor devices through nanomaterials. Nanomaterials offer unique advantages for pathogen detection. The nanoscale and high specific surface area allows for more effective interaction with pathogenic agents, enhancing the sensitivity and selectivity of the biosensors. Finally, biosensors’ capability to functionalize with specific molecules such as antibodies or nucleic acids facilitates the specific detection of the target pathogens.

show abstract

“…On the other hand, peptides have biocompatible, binding affinity, and selective biological properties [20]. A peptide that forms part of a protein sequence may exhibit properties equivalent to those of the protein.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, peptides have biocompatible, binding affinity, and selective biological properties [20].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

2023

View full text Add to dashboard Cite

To electrochemically measure human myeloid leukemia cells (K562 cells), we constructed a probe consisting of peptide/single‐strand (ss) DNA. Ac‐H6Y4C with an acetylated N‐terminal of peptide was used to enhance the probe to allow electrode responses that could detect target cells. A ss‐DNA was selected as the target cell recognition moiety. The probe exhibits properties that combine the functionalities of both DNA and peptides. The measurement principle is based on changes in the peak currents of the peptide moieties that are caused by interactions between the ss‐DNA and target cells. The peak currents were proportional to the concentration of K 562 cells that ranged from 10 to 2,000 cells/mL with a LOD of 3 cells/mL.

show abstract

Electrochemical nanobiosensors equipped with peptides: a review

Cited by 20 publications

References 151 publications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Emerging Applications of Nanobiosensors in Pathogen Detection in Water and Food

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

Contact Info

Product

Resources

About