Electrochemical On‐site Amino Acids Detection of Maple Syrup Urine Disease Using Vertically Aligned Nickel Nanowires

García-Carmona, Laura; González, Marı́a Cristina; Escarpa, Alberto

doi:10.1002/elan.201800103

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…The use of metal nanowires (NWs) gives some advantages in clinical diagnosis such as simplicity, rapid sensor response and short total analysis time, and low sample consumption. Recently, García-Carmona et al [77] demonstrated the fabrication of vertically aligned nickel nanowires-based electrochemical sensors (v-NiNWs) for fast determination of b AA, aiming to noninvasive screening of MSUD. v-NiNWs.…”

Section: Metal Nanoparticles and B Aa Electrochemical (Bio)sensingmentioning

confidence: 99%

Electrochemical (Bio)Sensing of Maple Syrup Urine Disease Biomarkers Pointing to Early Diagnosis: A Review

Karastogianni

Girousi

2020

Applied Sciences

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Metabolic errors are inherited diseases, where genetic defects prevent a metabolic path, ending up in enzyme malfunction. In correspondence to its remaining or plenitude fall of enzymatic potency, there is an amassment of dangerous metabolites near the metabolic bar and/or a dearth of necessary products, inducing a certain disease. These metabolic errors may include deviations such as point mutations, expunctions or interferences, or further complicated genomic disorders. Based on these facts, maple syrup urine disease (MSUD) is a scarce metabolic disease, generated by huge concentrations of branched-chain amino acids (b AAs), i.e., leucine, isoleucine, and valine. In this situation, these large amounts of b AAs provoke abnormalities such as liver failure, neurocognitive dysfunctions, and probably death. To overpass those problems, it is crucial to implement a timely and agile diagnosis at the early stages of life in view of their immutable consequence on neonates. Thus, this review will describe MSUD and b AAs analysis based on electrochemical (bio)sensing.

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Section: Metal Nanoparticles and B Aa Electrochemical (Bio)sensingmentioning

confidence: 99%

Electrochemical (Bio)Sensing of Maple Syrup Urine Disease Biomarkers Pointing to Early Diagnosis: A Review

Karastogianni

Girousi

2020

Applied Sciences

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“…The analysis of several diseases by non‐invasive methods is a good alternative in clinical analysis since it implies less discomfort for the patient. For this type of analysis, the main matrices used are urine and saliva . Among the compounds that act as biomarkers for disease diagnosis are UA, creatinine, and glucose, which can be found in urine samples.…”

Section: Introductionmentioning

confidence: 99%

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

et al. 2020

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A disposable electrochemical paper‐based analytical device was constructed based on use of sequential analysis with multiplexed working electrodes and applied for the determination of glucose, creatinine, and uric acid. The device was constructed with 16 microfluidic channels, with 16 working electrodes arranged in four set with four components surrounding the sample injection hole. In addition, a commercial multiplexing module was used, which allowed for multiplexing of the 16 working electrodes. This design allowed for radial and homogeneous sample elution to each sensing spot for high throughput analysis. In the multiplexed determinations, distinct electrochemical procedures were employed for each analyte. Furthermore, each working electrode spot was modified to increase the respective analytical signals. For glucose detection, the sensor was based on electron mediation by ferrocenecarboxylic acid over the modified surface with glucose oxidase. The principle for creatinine detection was based on electrochemical reduction of non‐complexed Fe3+ in excess after complex formation between Fe3+ and creatinine in the chemical step. The anodic peak current responses for uric acid detection increased due to working electrode surface modification with carbon black nanoparticles. In the multiplexed analysis, the device provided limits of detection of 0.120 mmol L−1, 0.084 mmol L−1, and 0.012 mmol L−1 for glucose, creatinine, and uric acid, respectively. The developed device was successfully applied in the analyses of real urine samples.

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“…Amino acid analysis is an important technique in many pharmaceutical, medical, chemical, food research, and industrial areas [1][2][3]. Thus far, various analytical methods have been proposed to determine amino acids, including high-performance liquid chromatography, capillary electrophoresis, enzyme assay, and chemical sensors [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Usually, because of the weak light-absorptive property of amino acids, pre-or post-column derivatization with an appropriate labeling reagent is performed for determining amino acids with sensitivity and selectivity in HPLC with UV detection (HPLC-UV) and fluorescence detection (HPLC-FL) systems [9,10].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Amino Acids Based on the Measurement of Reduction Prepeak of Quinone Caused by Surplus Acid after Neutralization

et al. 2018

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In the voltammetric reduction of quinone, such as 3,5‐di‐tert‐butyl‐1,2‐benzoquinone (DBBQ), in the presence of HCl, a reduction prepeak of DBBQ caused by HCl can be observed on a voltammogram. Here, we have shown that the prepeak height of DBBQ is linearly decreased with increasing concentrations of amino acid in an electrolyte solution when this voltammetry is performed after neutralization between amino acids and excess HCl. This finding is based on a concept of acid‐base back titration of amino acids with excess HCl where surplus HCl gives a reduction prepeak of DBBQ. In this study, the reduction behaviors of DBBQ in the presence of amino acids and HCl have been provided to develop a novel electroanalytical method for the determining of amino acids. Electrochemical detection of 20 kinds of α‐amino acids, including less electroactive ones, has been achieved using a bare plastic formed carbon working electrode. Moreover, a flow injection analysis with electrochemical detection system using a glassy carbon working electrode is developed based on the present detection principle, and it has been applied to the determination of glutamine in an L‐glutamine granulated powder.

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Electrochemical On‐site Amino Acids Detection of Maple Syrup Urine Disease Using Vertically Aligned Nickel Nanowires

Cited by 8 publications

References 33 publications

Electrochemical (Bio)Sensing of Maple Syrup Urine Disease Biomarkers Pointing to Early Diagnosis: A Review

Electrochemical (Bio)Sensing of Maple Syrup Urine Disease Biomarkers Pointing to Early Diagnosis: A Review

New Disposable Electrochemical Paper‐based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

Voltammetric Determination of Amino Acids Based on the Measurement of Reduction Prepeak of Quinone Caused by Surplus Acid after Neutralization

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