Electrochemical Preparation of a Molecularly Imprinted Polypyrrole-modified Pencil Graphite Electrode for Determination of Ascorbic Acid

Abstract

A molecularly imprinted polymer (MIP) polypyrrole (PPy)-based film was fabricated for the determination of ascorbic acid. The film was prepared by incorporation of a template molecule (ascorbic acid) during the electropolymerization of pyrrole onto a pencil graphite electrode (PGE) in aqueous solution using a cyclic voltammetry method. The performance of the imprinted and non-imprinted (NIP) films was evaluated by differential pulse voltammetry (DPV). The effect of pH, monomer and template concentrations, electropolymerization cycles and interferents on the performance of the MIP electrode was investigated and optimized. The molecularly imprinted film exhibited a high selectivity and sensitivity toward ascorbic acid. The DPV peak current showed a linear dependence on the ascorbic acid concentration and a linear calibration curve was obtained in the range of 0.25 to 7.0 mM of ascorbic acid with a correlation coefficient of 0.9946. The detection limit (3σ) was determined as 7.4×10−5 M (S/N=3). The molecularly-imprinted polypyrrole-modified pencil graphite electrode showed a stable and reproducible response, without any influence of interferents commonly existing in pharmaceutical samples. The proposed method is simple and quick. The PPy electrodes have a low response time, good mechanical stability and are disposable simple to construct.

Synthesis by precipitation polymerization of a molecularly imprinted polymer membrane for the potentiometric determination of sertraline in tablets and biological fluids

“…1 This procedure can be accomplished via either reversible covalent bonding or non-covalent interactions between monomers and imprinting molecules. 2 After removal of the template, the polymer can be used as a selective binding medium for the print molecule or structurally related compounds. 3 Various types of electrosynthesized polymers based on molecular imprinting have been reported in the literature, including polypyrrole 4 and a copolymer of aniline with o-phenylenediamine.…”

Multi-templated Pb-Zn-Hg Ion Imprinted Polymer for the Selective and Simultaneous Removal of Toxic Metallic Ions from Wastewater

“…However, these methods have been reported to have some challenges such as high cost (Chee, Wong, & Lee, 1996), require a trained personnel (Ahmad, Mohd, & Universiti, 2015), high energy consumption (Perdew, Burke, & Ernzerhof, 1996), use of large quantities of chemicals (Atassi, Tally, & Ismail, 2008), poor removal rate to meet the pollution control limits (Piletska et al, 2008), low mechanical strength of adsorbents (Özcan, Sahin, & Sahin, 2008), weak hydrothermal stability (Firouzzare & Wang, 2012), poor selectivity (O'Mahony, Molinelli, Nolan, Smyth, & Mizaikoff, 2005) and time consuming (Mijangos et al, 2006). or their combinations have been employed to eliminate the challenges of previous traditional methods (C.-Y.…”

Electrochemical Flow Injection Determination of Ascorbic Acid in Fruit Samples Employing a Graphene-Polyaniline Electrode

“…Nevertheless, gastric irritation due to an AA excess can generate the metabolic product of AA, causing renal problems [3]. Many analytical techniques have been proposed for the estimation of AA content such as chromatography [4], fluorimetry [5], spectrophotometry [6], redox titration [7], and electrochemical method [8]. Among these methods, the electrochemical method presents some advantages such as high sensitivity and ability to assay the electroactive species in coloured samples.…”

“…Some chemically modified electrodes have been widely applied to decrease the oxidation overpotential and the detection of AA at the electrode surface/solution interface [1], [8], [9]. However, the electrodes usually lost their electroactivity easily by the unstable immobilisation of modifiers, resulted in unreliable determinations, thus causing inaccuracy in the measurement.…”

“…The most simple and cost-effective flow injection Manuscript received April 19, 2015; revised June 17, 2015. The authors are with the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand (e-mail: view-wow@hotmail.com, orn.arquero@gmail.com, scislwrn@gmail.com, suriyacmu@yahoo.com).(FI) manifold could be operated with the electrochemical detection.Some chemically modified electrodes have been widely applied to decrease the oxidation overpotential and the detection of AA at the electrode surface/solution interface [1], [8], [9]. However, the electrodes usually lost their electroactivity easily by the unstable immobilisation of modifiers, resulted in unreliable determinations, thus causing inaccuracy in the measurement.…”