Electrochemical Property and Cell Toxicity of Gold Electrode Modified by Monolayer PAMAM Encapsulated Gold Nanorods

Zhang, Xueqing; Pan, Bifeng; Wang, Kan; Ruan, Jing; Yang, Haipeng; He, Rong; Cui, Daxiang

doi:10.5101/nbe.v2i3.p182-188

Cited by 11 publications

(8 citation statements)

References 29 publications

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“…The electrochemical response of the ZnO-NF-based sensor vs. a Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (500 nM to 1.5 mM). The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea [11].…”

Section: Introductionmentioning

confidence: 88%

Electrochemical Study of Pb(II) in Present of Each Ascorbic Acid, Glucose, Urea and Uric Acid Using Blood Medium as an Electrolyte

Radhi¹,

Albakry²,

Jassim³

et al. 2016

Nano BioMed ENG

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Electrochemical of redox current peaks of lead sulphate PbSO 4 was studied in blood medium in present of different reagents such as ascorbic acid (AA), glucose, urea, and uric acid using cyclic voltammetric technique at glassy carbon electrode (GCE). It was found that Pb(II) ions in aqueous electrolyte (0.1 M KCl) have oxidation current peak at -540 mV and reduction current peak at -600 mV. But, it was different electrochemical properties of the redox current peaks of Pb(II) ions in blood medium, the reduction current peak was disappearing and the oxidation current peak was enhanced. Also, in the different reagents (glucose, AA, urea and uric acid) causes an enhancement of the oxidation current peak and reducing of the reduction current peak or disappearing. It means that the reagents (glucose, AA, urea and uric acid) were oxidative effective in the blood component for the lead ions in the damage the blood cells.

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Section: Introductionmentioning

confidence: 88%

Electrochemical Study of Pb(II) in Present of Each Ascorbic Acid, Glucose, Urea and Uric Acid Using Blood Medium as an Electrolyte

Radhi¹,

Albakry²,

Jassim³

et al. 2016

Nano BioMed ENG

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“…The use of structurally modified gold nanoparticles is less toxic to normal tissue during delivery, and at the molecular level, could traverse biologic barriers and preferentially accumulate in cancer cells [13][14].…”

Section: Introductionmentioning

confidence: 99%

The radiosensitization of melanoma cells by gold nanorods irradiated with MV X-ray

Xu¹,

Luo²,

Pang³

et al. 2012

Nano BioMed ENG

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Melanoma is known to be radioresistant and traditional treatments have been intractable, and therefore, novel approaches are required to improve therapeutic efficacy. Gold nanoparticles (GNPs) have been explored as radiosensitizers, while most of the research in the area has focused on the enhancement occurred in the kilovoltage (kV ) range. The present study investigated the possible application and biological mechanism of gold nanorods (GNRs) for sensitization at clinically relevant MV X-ray energies. A375 melanoma cells were treated by gold nanorods (GNRs) with or without irradiation. The anti-proliferative impacts of the treatments were measured by MTT assay. The cellular uptake and intracellular localization were analyzed by transmission electron microscopy. Radiosensitizing effects were determined by a colony formation assay. Apoptosis and cell cycle data were measured by flow cytometry. DNA damage was estimated by γ-H2AX expression measured with immunofluorescent staining.Results showed that the addition of GNRs enhanced the radiosensitivity of A375 cells with a dose-modifying factor (DMFSF2) of 1.14, increasing more radiation-induced DNA double-strand breaks and apoptosis. DNA flow cytometric analysis indicated that GNRs plus irradiation significantly induced G2/M phase arrest in A375 cells.In Conclusions: GNRs could sensitize melanoma A375 cells to 6 MV X-ray irradiation, and this was mainly through increasing the DNA doublestrand breaks, in addition to the induction of a higher proportion of cells within the G2/M phase. The interaction of GNRs and high energy commonly used in the clinic may provides another rational for the potential application of GNRs in the treatment of cancer.. Citation: W. Xu, et al. The radiosensitization of melanoma cells by gold nanorods irradiated with MV X-ray .

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“…The use of structurally modified GNR is less toxic to normal tissue during delivery. At the molecular level, GNR could traverse biologic barriers and preferentially accumulate in cancer cells [9,[29][30][31]. Targeting GNR to a specific site is a critical aspect of bioimaging when used as a contrast agent.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

Conjusteau¹

2015

J Nanomed Nanotechnol

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Gold nanorods with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide on the surface of the nanorods with 16-mercaptohexadecanoic acid and metoxy-poly(ethylene glycol)-thiol, and the monoclonal antibody HER2. The physical chemistry properties of the conjugates were monitored through optical and zeta-potential measurements to confirm surface chemistry changes. The efficiency of the modifications was quantified through measurement of the average number of antibodies per gold nanorod. The conjugates were investigated for different cells lines: BT-474, MCF7, MCF10, MDCK, and fibroblast. The results show successful cell accumulation of the gold nanorod HER2 conjugates in cells with HER2 overexpression. Incubation of the complexes in heparinized mouse blood demonstrated the low aggregation of the metallic particles through stability of the spectral properties, as verified by UV/VIS spectrometry. Cytotoxicity analysis with LDH release and MTT assay confirms strong targeting and retention of functional activity of the antibody after their conjugation with gold nanorods. Silver staining confirms efficient specific binding to BT-474 cells even in cases where the nanorod complexes were incubated in heparinized mouse blood. This is confirmed through in vivo studies where, following intravenous injection of gold nanorod complexes, silver staining reveals noticeably higher rates of specific binding in mouse tumors than in healthy liver.The conjugates are reproducible, have strong molecular targeting capabilities, have long term stability in vivo and can be used in pre-clinical applications. The conjugates can also be used for molecular and optoacoustic imaging, quantitative sensing of biological substrates, and photothermal therapy.

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Electrochemical Property and Cell Toxicity of Gold Electrode Modified by Monolayer PAMAM Encapsulated Gold Nanorods

Cited by 11 publications

References 29 publications

Electrochemical Study of Pb(II) in Present of Each Ascorbic Acid, Glucose, Urea and Uric Acid Using Blood Medium as an Electrolyte

Electrochemical Study of Pb(II) in Present of Each Ascorbic Acid, Glucose, Urea and Uric Acid Using Blood Medium as an Electrolyte

The radiosensitization of melanoma cells by gold nanorods irradiated with MV X-ray

Biocompatible Gold Nanorod Conjugates for Preclinical Biomedical Research

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