Electrochemical Proteus vulgaris whole cell urea sensor in synthetic urine

Morales-Cruz, Myreisa; Solis-Marcano, Nadja E.; Binder, Claudia; Priest, Craig; Cabrera, Carlos R.

doi:10.1016/j.crbiot.2019.07.002

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…Due to the poisoning phenomenon, great effect on sensibility can be found when performing continuous glucose concentration tests repeatedly. In the case of electro-oxidation of urea, it has high sensibility with platinum and PBS …”

Section: Resultsmentioning

confidence: 99%

“…Over the years, research on urea sensors has flourished, attributable to the importance of these devices in different fields such as clinical determination, ecological inspection, and agro-food investigation . Urea levels range from 2.6 to 6.5 mM in human blood and are 342 ± 67 mM in 490–2690 mL of human urine . Renal failure, urinary tract obstruction, gastrointestinal bleeding, lack of hydration, burns, and pain are associated with higher levels of urea in the blood and lower levels of urea due to hepatic failure, nephritis, and cachexia.…”

Section: Introductionmentioning

confidence: 99%

“…1 Urea levels range from 2.6 to 6.5 mM in human blood 2 and are 342 ± 67 mM in 490−2690 mL of human urine. 3 Renal failure, urinary tract obstruction, gastrointestinal bleeding, lack of hydration, burns, and pain are associated with higher levels of urea in the blood and lower levels of urea due to hepatic failure, nephritis, and cachexia. Numerous analytical techniques have been used for the determination of urea.…”

Section: Introductionmentioning

confidence: 99%

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New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

Fatema

Jung

Liu

et al. 2020

ACS Biomater. Sci. Eng.

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In the present study, electrochemical sensing for urea was proposed utilizing graphene-based quaternary nanocomposites YInWO4-G-SiO2 (YIWGS). These YIWGS nanocomposites were utilized due to their exceptionally delicate determination of urea with the lowest detection limit (0.01 mM). These YIWGS composites were developed through a simple self-assembly method. From physical characterization, we found that the YIWGS composites are crystalline in nature (powdered X-ray diffraction), and Fourier transform infrared (FTIR) spectroscopy analysis provided the surface functionality and bonding. Scanning electron microscopy (SEM) studies indicated the morphology characteristics of the as-synthesized composites and the high-resolution transmission electron microscopy (HRTEM) image supported the formation of cubic or hexagonal morphology of the YIW nanocomposites. The YIWGS sensor showed a great electroanalytical sensing performance of 0.07 mM urea with a sensitivity of 0.06 mA cm–2, an expansive linear range of 0.7–1.5 mM with a linear response (R2 1/4 0.99), and an eminent reaction time of around 2 s. It also displayed a good linear response toward urea with negligible interferences from normal coinciding species in urine samples.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

Fatema

Jung

Liu

et al. 2020

ACS Biomater. Sci. Eng.

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“…Furthermore, P. vulgaris was used on the surface of electrode of platinum to convert urea into ammonia which prevents blockage on the surface of the platinum or also prevents its oxidation reaction capability. The proposed research serves as a medium for the sensing, tracking, in vivo urine and point‐of‐care urea detection of waste water [ 32 ].…”

Section: Classification Of Urea Biosensorsmentioning

confidence: 99%

Recent advancements in urea biosensors for biomedical applications

Singh

Sharma

Singh

2021

IET Nanobiotechnology

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The quick progress in health care technology as a recurrent measurement of biochemical factors such as blood components leads to advance development and growth in biosensor technology necessary for effectual patient concern. The review wok of authors present a concise information and brief discussion on the development made in the progress of potentiometric, field effect transistor, graphene, electrochemical, optical, polymeric, nanoparticles and nanocomposites based urea biosensors in the past two decades. The work of authors is also centred on different procedures/methods for detection of urea by using amperometric, potentiometric, conductometric and optical processes, where graphene, polymer etc. are utilised as an immobilised material for the fabrication of biosensors. Further, a comparative revision has been accomplished on various procedures of urea analysis using different materials-based biosensors, and it discloses that electrochemical and potentiometric biosensor is the most promise one among all, in terms of rapid response time, extensive shelf life and resourceful design.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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“…Cyclic olefin copolymer (COC) is an amorphous thermoplastic composed of ethylene and norbornene polymers at varying ratios, utilized for small electronics due to its performance characteristics such as optical transparency, thermostability, and chemical resistance [13][14][15]. In the last few years, there has been an increase interest in the utilization of COC as biosensor substrate, specifically in microfluidics due to its micropatterning ease and biocompatibility [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

et al. 2021

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A fast PCR-assisted impedimetric biosensor was developed for the selective detection of the clbN gene from the polyketide synthase (pks) genomic island in real Escherichia coli samples. This genomic island is responsible for the production of colibactin, a harmful genotoxin that has been associated with colorectal cancer. The experimental protocol consisted of immobilizing the designated forward primer onto an Au electrode surface to create the sensing probe, followed by PCR temperature cycling in blank, positive, and negative DNA controls. Target DNA identification was possible by monitoring changes in the system's charge transfer resistance values (R ct ) before and after PCR treatment through electrochemical impedance spectroscopy (EIS) analysis. Custom-made, flexible gold electrodes were fabricated using chemical etching optical lithography. A PCR cycle study determined the optimum conditions to be at 6 cycles providing fast results while maintaining a good sensitivity. EIS data for the DNA recognition process demonstrated the successful distinction between target interaction resulting in an increase in resistance to charge transfer (R ct ) percentage change of 176% for the positive DNA control vs. 21% and 20% for the negative and non-DNA-containing controls, respectively. Results showed effective fabrication of a fast, PCR-based electrochemical biosensor for the detection of pks genomic island with a calculated limit of detection of 17 ng/μL.

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Electrochemical Proteus vulgaris whole cell urea sensor in synthetic urine

Cited by 9 publications

References 39 publications

New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

Recent advancements in urea biosensors for biomedical applications

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

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Electrochemical Proteus vulgaris whole cell urea sensor in synthetic urine

Cited by 9 publications

References 39 publications

New Design of Active Material Based on YInWO4-G-SiO2for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

New Design of Active Material Based on YInWO4-G-SiO2for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

Recent advancements in urea biosensors for biomedical applications

PCR-assisted impedimetric biosensor for colibactin-encoding pks genomic island detection in E. coli samples

Contact Info

Product

Resources

About

New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity

New Design of Active Material Based on YInWO₄-G-SiO₂for a Urea Sensor and High Performance for Nonenzymatic Electrical Sensitivity