Electrochemical quercetin sensor based on a nanocomposite consisting of magnetized reduced graphene oxide, silver nanoparticles and a molecularly imprinted polymer on a screen-printed electrode

Abstract

An electrochemical quercetin (QR) sensor is described that is based on the use of magnetic reduced graphene oxide (MrGO) incorporated into a molecularly imprinted polymer (MIP) on the surface of a screen-printed electrode (SPE). The MrGO consists of reduced graphene oxide (rGO), magnetite (FeO) and silver nanoparticles (Ag). The analyte (QR) is electrostatically adsorbed on the surface of the MrGO. Finally, the MIP was deposited via in-situ polymerization. The composite was characterized by X-ray diffraction, Fourier transform infrared spectroscopy and Vibrating sample magnetometry. The morphologies and electrochemical properties of different electrodes were characterized by Field emission scanning electron microscopy, Electrochemical impedance spectroscopy and differential pulse voltammetry. Under optimal conditions, the modified electrode has a linear response in the 20 nM to 250 μM QR concentration range. The limit of detection is 13 nM (at an S/N ratio of 3). The electrode is selective, stable, regenerable and reliable. It was applied to the determination of QR in spiked pharmaceutical samples and gave satisfactory results. Graphical abstract Schematic presentation of a method for sensing quercetin. It is based on the use of screen printed electrode modified with magnetized reduced graphene oxide and a molecularly imprinted polymer.

Novel plant flavonoid electrochemical sensor based on in-situ and controllable double-layered membranes modified electrode

“…The absorbance bands of rGO-Au were little changed ( Fig 3c ). Many adsorption peaks located between 2000 cm -1 and 1000 cm -1 presence in spectrum of rGO-Au/MIPs ( Fig 3d ) can be classified to the organic groups introduced by DMY, EDGMA, and AM in MIPs [ 18 ].…”

“…Due to good recognition and enrichment abilities to target molecules together with many other advantages including stability, inexpensive, simplicity, and reusability compare with natural biological receptors, molecularly imprinted polymers (MIPs) have been regarded as ideal artificial recognition elements to improve the measuring selectivity for electrochemical sensor in complicated matrices [ 15 – 17 ]. Numerous MIPs modified electrodes were fabricated and used widely in determination of quercetin, amyloid-β protein, chlorpyrifos, and azithromycin [ 18 – 21 ], etc. However, drawbacks of slow mass transfer, low binding capacity, and poor binding kinetics resulted from these organic materials.…”

“…Based on our previous researches in Gr-based nanocomposite and MIPs [ 10 , 11 , 18 ], this investigation aims at fabricating a novel DMY electrochemical sensor based on double-layered membranes consisting of rGO-Au nanocomposite and MIPs modified GCE (labeled as GCE│rGO-Au/MIPs). Both rGO-Au and MIPs membranes were formed on GCE via in-situ electrochemical reduction and polymerization processes step by step.…”

Point-of-Care Diagnostics: Molecularly Imprinted Polymers and Nanomaterials for Enhanced Biosensor Selectivity and Transduction

“…Another instance utilizing AgNPs takes advantage of their enhanced electrical conductivity to overcome a MIP's insulating nature. The reported ECM sensor also makes use of MNPs and reduced GO resulting in a nanocomposite modification to the electrode (122). The MIPs were arranged in a thin layer ( Fig.…”

Graphene nanocrystals in CO2photoreduction with H2O for fuel production

“…43 In addition, the composites containing graphene and Ag NPs can be explored for use as glucose and quercetin sensors. 44,45…”